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. 1982 Sep;47(1):115–121.

Inhibition of proliferation of a murine myeloma cell line and mitogen-stimulated B lymphocytes by the antibiotic amphotericin B (Fungizone).

E V Walls, J E Kay
PMCID: PMC1555504  PMID: 6981585

Abstract

Amphotericin B at the concentration normally used for routine suppression of fungal infection in tissue culture strongly inhibits the proliferation of NS1/1 myeloma cells and the LPS-induced activation of B lymphocytes from mouse spleen. The proliferation of T lymphocytes induced by concanavalin A (Con A) was less affected by the antibiotic, indicating that B-lymphocyte proliferation was preferentially inhibited. The unexpected sensitivity of B-lymphoid cells to amphotericin B precludes its use as an anti-fungal agent in the production of hybridomas from fusions between these cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abo T., Balch C. M. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol. 1981 Sep;127(3):1024–1029. [PubMed] [Google Scholar]
  2. Boxer L. A., Ingraham L. M., Allen J., Oseas R. S., Baehner R. L. Amphotericin-B promotes leukocyte aggregation of nylon-wool-fiber-treated polymorphonuclear leukocytes. Blood. 1981 Sep;58(3):518–523. [PubMed] [Google Scholar]
  3. Hammarström L., Smith E. Mitogenic properties of polyene antibiotics for murine B cells. Scand J Immunol. 1976;5(1-2):37–43. doi: 10.1111/j.1365-3083.1976.tb02990.x. [DOI] [PubMed] [Google Scholar]
  4. Kraemer K. H., Waters H. L., Buchanan J. K. Survival of human lymphoblastoid cells after DNA damage measured by growth in microtiter wells. Mutat Res. 1980 Sep;72(2):285–294. doi: 10.1016/0027-5107(80)90043-3. [DOI] [PubMed] [Google Scholar]
  5. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  6. Lemke H., Hammerling G. J., Hohmann C., Rajewsky K. Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse. Nature. 1978 Jan 19;271(5642):249–251. doi: 10.1038/271249a0. [DOI] [PubMed] [Google Scholar]
  7. Olds G. R., Stewart S. J., Ellner J. J. Amphotericin B-induced resistance to Schistosoma mansoni. J Immunol. 1981 May;126(5):1667–1670. [PubMed] [Google Scholar]
  8. Shirley S. F., Little J. R. Immunopotentiating effects of amphotericin B. I. Enhanced contact sensitivity in mice. J Immunol. 1979 Dec;123(6):2878–2882. [PubMed] [Google Scholar]
  9. Shirley S. F., Little J. R. Immunopotentiating effects of amphotericin B. II. Enhanced in vitro proliferative responses of murine lymphocytes. J Immunol. 1979 Dec;123(6):2883–2889. [PubMed] [Google Scholar]
  10. Stewart S. J., Spagnuolo P. J., Ellner J. J. Generation of suppressor T lymphocytes and monocytes by amphotericin B. J Immunol. 1981 Jul;127(1):135–139. [PubMed] [Google Scholar]

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