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. 1982 Sep;47(1):175–181.

The effect of human monocytes and macrophages on lymphocyte proliferation.

J H Passwell, M Levanon, J Davidsohn, F Kohen, B Ramot
PMCID: PMC1555515  PMID: 6956545

Abstract

Human monocytes suppressed both the phytohaemagglutin (PHA) or antigen-induced lymphocyte proliferative response, when the monocyte: lymphocyte ratio was increased or when the monocytes were stimulated with zymosan or endotoxin. The effect of monocytes on autologous lymphocyte proliferation was compared with that of macrophages obtained by culturing monocytes in vitro for 7 days. The lymphocyte proliferative responses were increased in the presence of macrophages, however, neither increasing their number nor stimulation by zymosan or endotoxin altered the autologous lymphocyte proliferative response to PHA or purified protein derivative (PPD). The PGE2 concentration in the medium of both the cultured monocytes or macrophages activated by zymosan and endotoxin rose markedly without a corresponding suppressor effect on lymphocyte proliferation in the presence of macrophages in the culture. Thus it seems that while PGE2 is a useful marker of mononuclear phagocyte activation, other molecular species are of importance in determining the lymphocyte proliferative response to mitogens and antigens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Calderon J., Unanue E. R. Two biological activities regulating cell proliferation found in cultures of peritoneal exudate cells. Nature. 1975 Jan 31;253(5490):359–361. doi: 10.1038/253359a0. [DOI] [PubMed] [Google Scholar]
  2. Fischer A., Durandy A., Griscelli C. Role of prostaglandin E2 in the induction of nonspecific T lymphocyte suppressor activity. J Immunol. 1981 Apr;126(4):1452–1455. [PubMed] [Google Scholar]
  3. Goldstein I. M., Malmsten C. L., Kindahl H., Kaplan H. B., Rådmark O., Samuelsson B., Weissmann G. Thromboxane generation by human peripheral blood polymorphonuclear leukocytes. J Exp Med. 1978 Sep 1;148(3):787–792. doi: 10.1084/jem.148.3.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goodwin J. S., Messner R. P., Bankhurst A. D., Peake G. T., Saiki J. H., Williams R. C., Jr Prostaglandin-producing suppressor cells in Hodgkin's disease. N Engl J Med. 1977 Nov 3;297(18):963–968. doi: 10.1056/NEJM197711032971802. [DOI] [PubMed] [Google Scholar]
  5. Gordon S., Todd J., Cohn Z. A. In vitro synthesis and secretion of lysozyme by mononuclear phagocytes. J Exp Med. 1974 May 1;139(5):1228–1248. doi: 10.1084/jem.139.5.1228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hersh E. M., Harris J. E. Macrophage-lymphocyte interaction in the antigen-induced blastogenic response of human peripheral blood leukocytes. J Immunol. 1968 Jun;100(6):1184–1194. [PubMed] [Google Scholar]
  7. Humes J. L., Bonney R. J., Pelus L., Dahlgren M. E., Sadowski S. J., Kuehl F. A., Jr, Davies P. Macrophages synthesis and release prostaglandins in response to inflammatory stimuli. Nature. 1977 Sep 8;269(5624):149–151. doi: 10.1038/269149a0. [DOI] [PubMed] [Google Scholar]
  8. Karnovsky M. L., Lazdins J. K. Biochemical criteria for activated macrophages. J Immunol. 1978 Sep;121(3):809–813. [PubMed] [Google Scholar]
  9. Keller R. Major changes in lymphocyte proliferation evoked by activated macrophages. Cell Immunol. 1975 Jun;17(2):542–551. doi: 10.1016/s0008-8749(75)80058-x. [DOI] [PubMed] [Google Scholar]
  10. Kurland J. I., Bockman R. Prostaglandin E production by human blood monocytes and mouse peritoneal macrophages. J Exp Med. 1978 Mar 1;147(3):952–957. doi: 10.1084/jem.147.3.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Novogrodsky A., Stenzel K. H., Rubin A. L. Stimulation of human peripheral blood lymphocytes by periodate, galactose oxidase, soybean agglutinin, and peanut agglutinin: differential effects of adherent cells. J Immunol. 1977 Mar;118(3):852–857. [PubMed] [Google Scholar]
  12. Passwell J. H., Dayer J. M., Merler E. Increased prostaglandin production by human monocytes after membrane receptor activation. J Immunol. 1979 Jul;123(1):115–120. [PubMed] [Google Scholar]
  13. Passwell J., Rosen F. S., Merler E. The effect of Fc fragments of IgG on human mononuclear cell responses. Cell Immunol. 1980 Jul 1;52(2):395–403. doi: 10.1016/0008-8749(80)90360-3. [DOI] [PubMed] [Google Scholar]
  14. Rinehart J. J., Orser M., Kaplan M. E. Human monocyte and macrophage modulation of lymphocyte proliferation. Cell Immunol. 1979 Apr;44(1):131–143. doi: 10.1016/0008-8749(79)90034-0. [DOI] [PubMed] [Google Scholar]
  15. Rode H. N., Gordon J. Macrophages in the mixed leukocyte culture reaction (MLC). Cell Immunol. 1974 Jul;13(1):87–94. doi: 10.1016/0008-8749(74)90229-9. [DOI] [PubMed] [Google Scholar]
  16. Schechter G. P., Soehnlen F. Monocyte-mediated inhibition of lymphocyte blastogenesis in Hodgkin disease. Blood. 1978 Aug;52(2):261–271. [PubMed] [Google Scholar]
  17. Schmidtke J. R., Hatfield S. Activation of purified human thymus-derived (T) cells by mitogens. II. Monocyte- macrophage potentiation of mitogen-induced DNA synthesis. J Immunol. 1976 Feb;116(2):357–362. [PubMed] [Google Scholar]
  18. Seeger R. C., Oppenheim J. J. Synergistic interaction of macrophages and lymphocytes in antigen-induced transformation of lymphocytes. J Exp Med. 1970 Jul 1;132(1):44–65. doi: 10.1084/jem.132.1.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stobo J. D. Immunosuppression in man: suppression by macrophages can be mediated by interactions with regulatory T cells. J Immunol. 1977 Sep;119(3):918–924. [PubMed] [Google Scholar]
  20. Territo M. C., Cline M. J. Monocyte function in man. J Immunol. 1977 Jan;118(1):187–192. [PubMed] [Google Scholar]
  21. Unanue E. R. The regulation of lymphocyte functions by the macrophage. Immunol Rev. 1978;40:227–255. doi: 10.1111/j.1600-065x.1978.tb00408.x. [DOI] [PubMed] [Google Scholar]
  22. Wing E. J., Remington J. S. Activated macrophages pre-incubated in vitro enhance rather than suppress mitogen-stimulated lymphocyte transformation. Immunology. 1980 Jun;40(2):239–246. [PMC free article] [PubMed] [Google Scholar]

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