Abstract
Rats were instilled intrabronchially with 1 mg UICC amosite suspended in 0.2 mL of filtered saline; control animals received the saline instillation only. Five animals from each group were killed on various days after instillation, up to day 128/129. Total retrieved cell counts and differential cell analysis were performed from lung and pleural lavages. In particular, the appearance of peroxidase-positive macrophages (PPM) as indicators of newly arrived macrophages was investigated. Polymorphonuclear cells (PMN) and PPMs in lung lavages increased in number 24 hr after amosite instillation and remained at increased levels until day 62. Alveolar macrophage numbers were significantly decreased after amosite instillation. There was only a very transient increase of PPMs and PMNs in the saline group. The number of PPMs in pleural lavage fluid was already increased 24 hr after amosite instillation. The pleural PPM increase was sustained throughout the study. No pleural reaction was seen in the saline instilled group. The inflammatory reactions indicated by the composition of the lavaged cells of the lung represent the in vivo toxicity of intrabronchially instilled amosite. The stimulus for recruitment of PMNs and PPMs is different, since no PMN response was detected in the pleural space. It is suggested that the response of the pleural PPMs is caused by the early arrival of fibers at the pleural sites, which results in the recruitment of PPMs to this space by an unknown mechanism.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Beelen R. H., van't Veer M. B., Fluitsma D. M., Hoefsmit E. C. Identification of different peroxidatic activity patterns in human macrophages in vivo and in vitro. J Reticuloendothel Soc. 1978 Oct;24(4):351–362. [PubMed] [Google Scholar]
- Bowden D. H., Adamson I. Y. Adaptive responses of the pulmonary macrophagic system to carbon. I. Kinetic studies. Lab Invest. 1978 Apr;38(4):422–429. [PubMed] [Google Scholar]
- Bryks S., Bertalanffy F. D. Cytodynamic reactivity of the mesothelium. Pleural reaction to chrysotile asbestos. Arch Environ Health. 1971 Dec;23(6):469–472. doi: 10.1080/00039896.1971.10666037. [DOI] [PubMed] [Google Scholar]
- Christensen T. G., Hayes J. A. Endogenous peroxidase in the conducting airways of hamsters: morphologic evidence of synthesis and secretion. Am Rev Respir Dis. 1982 Mar;125(3):341–346. doi: 10.1164/arrd.1982.125.3.341. [DOI] [PubMed] [Google Scholar]
- Hillerdal G. The pathogenesis of pleural plaques and pulmonary asbestosis: possibilities and impossibilities. Eur J Respir Dis. 1980 Jun;61(3):129–138. [PubMed] [Google Scholar]
- KAPLOW L. S. SIMPLIFIED MYELOPEROXIDASE STAIN USING BENZIDINE DIHYDROCHLORIDE. Blood. 1965 Aug;26:215–219. [PubMed] [Google Scholar]
- Miller K. The effects of asbestos on macrophages. CRC Crit Rev Toxicol. 1978 Sep;5(4):319–354. doi: 10.3109/10408447809081010. [DOI] [PubMed] [Google Scholar]
- Sebastien P., Fondimare A., Bignon J., Monchaux G., Desbordes J., Bonnaud G. Topographic distribution of asbestos fibres in human lung in relation to occupational and non-occupational exposure. Inhaled Part. 1975 Sep;4(Pt 2):435–446. [PubMed] [Google Scholar]
- Springmeyer S. C., Altman L. C., Kopecky K. J., Deeg H. J., Storb R. Alveolar macrophage kinetics and function after interruption of canine marrow function. Am Rev Respir Dis. 1982 Mar;125(3):347–351. doi: 10.1164/arrd.1982.125.3.347. [DOI] [PubMed] [Google Scholar]
- Wang N. S. The preformed stomas connecting the pleural cavity and the lymphatics in the parietal pleura. Am Rev Respir Dis. 1975 Jan;111(1):12–20. doi: 10.1164/arrd.1975.111.1.12. [DOI] [PubMed] [Google Scholar]
- Yazicioglu S., Ilçayto R., Balci K., Sayli B. S., Yorulmaz B. Pleural calcification, pleural mesotheliomas, and bronchial cancers caused by tremolite dust. Thorax. 1980 Aug;35(8):564–569. doi: 10.1136/thx.35.8.564. [DOI] [PMC free article] [PubMed] [Google Scholar]