Skip to main content
PMC home page
Environmental Health Perspectives logoLink to Environmental Health Perspectives
. 1983 Sep;51:267–273. doi: 10.1289/ehp.8351267

The advantages and limitations of an in vivo test system for investigating the cytotoxicity and fibrogenicity of fibrous dusts.

S E Sykes, A Morgan, S R Moores, W Davison, J Beck, A Holmes
PMCID: PMC1569310  PMID: 6315369

Abstract

The acute response of the rat lung to a range of fibrous materials has been investigated by bronchopulmonary lavage, at dose levels of 0.5 and 1.0 mg, 1 and 7 days after their administration by intratracheal instillation. The materials chosen for study included UICC chrysotile A, amosite, crocidolite and anthophyllite, and samples of S. African "long" amosite and glass fiber. In addition, the subacute response to 1, 2 and 3 mg of chrysotile and amosite has been studied at 50 and 100 days after instillation. In the acute phase at 1 day after instillation, the response to chrysotile was greater than that to any of the other materials, but by 7 days there was no gradation in the response to different dusts. In the subacute phase, cell recoveries were low, and it was not possible to assess the long-term cytotoxic or fibrogenic effects of amosite and chrysotile by analyses of lung washes, even though biochemical and histological methods indicated gross changes in lung pathology.

Full text

PDF
273

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown R. C., Chamberlain M., Griffiths D. M., Timbrell V. The effect of fibre size on the in vitro biological activity of three types of amphibole asbestos. Int J Cancer. 1978 Dec;22(6):721–727. doi: 10.1002/ijc.2910220614. [DOI] [PubMed] [Google Scholar]
  2. Harington J. S., Allison A. C., Badami D. V. Mineral fibers: chemical, physicochemical, and biological properties. Adv Pharmacol Chemother. 1975;12(0):291–402. doi: 10.1016/s1054-3589(08)60223-9. [DOI] [PubMed] [Google Scholar]
  3. Haslam P. L., Turton C. W., Lukoszek A., Salsbury A. J., Dewar A., Collins J. V., Turner-Warwick M. Bronchoalveolar lavage fluid cell counts in cryptogenic fibrosing alveolitis and their relation to therapy. Thorax. 1980 May;35(5):328–339. doi: 10.1136/thx.35.5.328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Light W. G., Wei E. T. Surface charge and hemolytic activity of asbestos. Environ Res. 1977 Feb;13(1):135–145. doi: 10.1016/0013-9351(77)90012-3. [DOI] [PubMed] [Google Scholar]
  6. McCullough B., Collins J. F., Johanson W. G., Jr, Grover F. L. Bleomycin-induced diffuse interstitial pulmonary fibrosis in baboons. J Clin Invest. 1978 Jan;61(1):79–88. doi: 10.1172/JCI108928. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Morgan A., Holmes A., Talbot R. J. The haemolytic activity of some fibrous amphiboles and its relation to their specific surface areas. Ann Occup Hyg. 1977 Jul;20(1):39–48. doi: 10.1093/annhyg/20.1.39. [DOI] [PubMed] [Google Scholar]
  8. Sykes S. E., Morgan A., Evans J. C., Evans N., Holmes A., Moores S. R. Use of an in vivo test system to investigate the acute and sub-acute responses of the rat lung to mineral dusts. Ann Occup Hyg. 1982;26(1-4):593–605. doi: 10.1093/annhyg/26.5.593. [DOI] [PubMed] [Google Scholar]
  9. Woodworth C. D., Mossman B. T., Craighead J. E. Comparative effects of fibrous and nonfibrous minerals on cells and liposomes. Environ Res. 1982 Feb;27(1):190–205. doi: 10.1016/0013-9351(82)90070-6. [DOI] [PubMed] [Google Scholar]

Articles from Environmental Health Perspectives are provided here courtesy of National Institute of Environmental Health Sciences

RESOURCES