Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1985 May;60(2):329–338.

Family distribution of anti-F(ab')2 antibodies in relatives of patients with systemic lupus erythematosus.

F Silvestris, R P Searles, A D Bankhurst, R C Williams
PMCID: PMC1577027  PMID: 3874025

Abstract

Recently we reported an inverse relationship between the levels of anti-F(ab')2 antibodies and disease activity in systemic lupus erythematosus (SLE). The present study focused on anti-F(ab')2 antibodies in unaffected relatives of SLE patients. Sixty sera from first degree family members from 11 SLE families and 49 sera from 8 control families were studied. Percentage of SLE family members with anti-DNA antibodies (15%) was higher than than control family sera (8%, P less than 0.05). Anti-F(ab')2 antibodies were measured using ELISA assays. The SLE family sera had higher amounts of anti-F(ab')2 antibodies than the normal control family group (P = 0.0051). In an effort to determine if anti-F(ab')2 antibodies found in high titres in the sera of some SLE family members had specificity for the F(ab')2 fragment of anti-DNA antibodies of the SLE relative patients, DNA-anti-DNA inhibition experiments were performed using anti-F(ab')2 prepared from the relative in parallel with anti-F(ab')2 prepared from normal controls with equivalent high titres of serum anti-F(ab')2. Inhibition exhibited by anti-F(ab')2 of first degree relatives was higher than that obtained from control normal donors (P less than 0.02). Such differences in inhibition were not recorded using a control tetanus toxoid-anti-tetanus toxoid assay. In direct binding ELISA experiments, peroxidase-conjugated anti-F(ab')2 antibodies from the same first degree relative showed high relative specificity against purified anti-DNA antibodies of his SLE proband when compared to those obtained against different anti-DNA antibodies isolated from unrelated SLE patients (P less than 0.001). Such a substantial difference was not observed in parallel experiments using peroxidase conjugated anti-F(ab')2 antibodies from normal controls unrelated to SLE subjects.

Full text

PDF
333

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdou N. I., Wall H., Lindsley H. B., Halsey J. F., Suzuki T. Network theory in autoimmunity. In vitro suppression of serum anti-DNA antibody binding to DNA by anti-idiotypic antibody in systemic lupus erythematosus. J Clin Invest. 1981 May;67(5):1297–1304. doi: 10.1172/JCI110158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Allen J. I., Searles R. P., Messner R. P., Bankhurst A. D. Antilymphocyte antibodies in systemic lupus erythematosus patients and their relatives: reactivity with nonhuman lymphocytes. Clin Immunol Immunopathol. 1978 Mar;9(3):371–378. doi: 10.1016/0090-1229(78)90109-5. [DOI] [PubMed] [Google Scholar]
  3. Arnett F. C., Shulman L. E. Studies in familial systemic lupus erythematosus. Medicine (Baltimore) 1976 Jul;55(4):313–322. doi: 10.1097/00005792-197607000-00003. [DOI] [PubMed] [Google Scholar]
  4. Birdsall H. H., Lidsky M. D., Rossen R. D. Anti-Fab' antibodies in rheumatoid arthritis. Measurement of the relative quantities incorporated in soluble immune complexes in sera and supernatants from cultured peripheral blood lymphocytes. Arthritis Rheum. 1983 Dec;26(12):1481–1492. doi: 10.1002/art.1780261210. [DOI] [PubMed] [Google Scholar]
  5. DeHoratius R. J., Messner R. P. Lymphocytotoxic antibodies in family members of patients with systemic lupus erythematosus. J Clin Invest. 1975 Jun;55(6):1254–1258. doi: 10.1172/JCI108044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DeHoratius R. J., Pillarisetty R., Messner R. P., Talal N. Anti-nucleic acid antibodies in systemic lupus erythematosus patients and their families. Incidence and correlation with lymphocytotoxic antibodies. J Clin Invest. 1975 Nov;56(5):1149–1154. doi: 10.1172/JCI108190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eaton R. B., Schnneider G., Schur P. H. Enzyme immunoassay for antibodies to native DNA. Specificity and quality of antibodies. Arthritis Rheum. 1983 Jan;26(1):52–62. doi: 10.1002/art.1780260109. [DOI] [PubMed] [Google Scholar]
  8. Elkon K. B., Walport M. J., Rynes R. I., Black C. M., Batchelor J. R., Hughes G. R. Circulating C1q binding immune complexes in relatives of patients with systemic lupus erythematosus. Arthritis Rheum. 1983 Jul;26(7):921–924. doi: 10.1002/art.1780260716. [DOI] [PubMed] [Google Scholar]
  9. Gallo R. C., Forde D. L. Familial chronic discoid lupus erythematosus and hypergammaglobulinemia. Arch Intern Med. 1966 May;117(5):627–631. [PubMed] [Google Scholar]
  10. HARBOE M., RAU B., AHO K. PROPERTIES OF VARIOUS ANTI-GAMMA-GLOBULIN FACTORS IN HUMAN SERA. J Exp Med. 1965 Apr 1;121:503–519. doi: 10.1084/jem.121.4.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heimer R., Wolfe L. D., Abruzzo J. L. IgM and IgG anti-F(ab')2 antibodies in rheumatoid arthritis and systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1298–1306. doi: 10.1002/art.1780251105. [DOI] [PubMed] [Google Scholar]
  12. Jerne N. K. Towards a network theory of the immune system. Ann Immunol (Paris) 1974 Jan;125C(1-2):373–389. [PubMed] [Google Scholar]
  13. Kávai M., Bányai A., Zsindely A., Sonkoly I., Szegedi G. Enzyme-linked immunosorbent assay for antibodies to native DNA in sera of patients with SLE. J Immunol Methods. 1982;48(2):169–175. doi: 10.1016/0022-1759(82)90191-0. [DOI] [PubMed] [Google Scholar]
  14. LEONHARDT T. FAMILY STUDIES IN SYSTEMIC LUPUS ERYTHEMATOSUS. Acta Med Scand. 1964;176:SUPPL 416–416:1+. [PubMed] [Google Scholar]
  15. MORTEO O. G., FRANKLIN E. C., MCEWEN C., PHYTHYON J., TANNER M. Studies of relatives of patients with systemic lupus erythematosus. Arthritis Rheum. 1961 Aug;4:356–363. doi: 10.1002/art.1780040404. [DOI] [PubMed] [Google Scholar]
  16. Miller K. B., Schwartz R. S. Familial abnormalities of suppressor-cell function in systemic lupus erythematosus. N Engl J Med. 1979 Oct 11;301(15):803–809. doi: 10.1056/NEJM197910113011502. [DOI] [PubMed] [Google Scholar]
  17. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  18. Nasu H., Chia D. S., Knutson D. W., Barnett E. V. Naturally occurring human antibodies to the F(ab')2 portion of IgG. Clin Exp Immunol. 1980 Nov;42(2):378–386. [PMC free article] [PubMed] [Google Scholar]
  19. Nasu H., Chia D. S., Taniguchi O., Barnett E. V. Characterization of anti-F(ab')2 antibodies in SLE patients. Evidence for cross-reacting auto-anti-idiotypic antibodies. Clin Immunol Immunopathol. 1982 Oct;25(1):80–90. doi: 10.1016/0090-1229(82)90167-2. [DOI] [PubMed] [Google Scholar]
  20. Natvig J. B. Heterogeneity of anti-gamma-globulin factors detected by pepsin-digested human gamma G-globulin. Acta Pathol Microbiol Scand. 1966;66(3):369–382. doi: 10.1111/apm.1966.66.3.369. [DOI] [PubMed] [Google Scholar]
  21. Nies K. M., Brown J. C., Dubois E. L., Quismorio F. P., Friou G. J., Terasaki P. I. Histocompatibility (HL-A) antigens and lymphocytotoxic antibodies in systemic lupus erythematosus (SLE). Arthritis Rheum. 1974 Jul-Aug;17(4):397–402. doi: 10.1002/art.1780170409. [DOI] [PubMed] [Google Scholar]
  22. OSTERLAND C. K., HARBOE M., KUNKEL H. G. Anti-gamma-globulin factors in human sera revealed by enzymatic splitting of anti-Rh antibodies. Vox Sang. 1963 Mar-Apr;8:133–152. doi: 10.1111/j.1423-0410.1963.tb03290.x. [DOI] [PubMed] [Google Scholar]
  23. Reinertsen J. L., Klippel J. H., Johnson A. H., Steinberg A. D., Decker J. L., Mann D. L. B-lymphocyte alloantigens associated with systemic lupus erythematosus. N Engl J Med. 1978 Sep 7;299(10):515–518. doi: 10.1056/NEJM197809072991004. [DOI] [PubMed] [Google Scholar]
  24. Searles R. P., Husby G., Messner P. Immunoglobulin class of antilymphocyte antibodies in systemic lupus erythematosus patients and their families. Acta Pathol Microbiol Scand C. 1977 Dec;85C(6):463–468. doi: 10.1111/j.1699-0463.1977.tb03669.x. [DOI] [PubMed] [Google Scholar]
  25. Silvestris F., Bankhurst A. D., Searles R. P., Williams R. C., Jr Studies of anti-F(ab')2 antibodies and possible immunologic control mechanisms in systemic lupus erythematosus. Arthritis Rheum. 1984 Dec;27(12):1387–1396. doi: 10.1002/art.1780271209. [DOI] [PubMed] [Google Scholar]
  26. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES