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. 1985 Feb;59(2):285–292.

Complement activating properties of complexes containing rheumatoid factor in synovial fluids and sera from patients with rheumatoid arthritis.

C J Elson, S D Carter, B J Cottrell, D G Scott, P A Bacon, T B Wallington
PMCID: PMC1577124  PMID: 3978872

Abstract

The relationship between complexes containing rheumatoid factor and complexes activating complement was examined in synovial fluids and sera from patients with rheumatoid arthritis (RA). In each case this was performed by quantifying the amount of rheumatoid factor bound by solid phase Fab'2 anti-C3 and/or solid phase conglutinin. Both anti-C3 coated and conglutinin coated microtitre plates bound high levels of complexes containing rheumatoid factor from sera of RA patients with vasculitis. Unexpectedly, these complexes were detected in synovial fluids from only a minority of RA patients with synovitis. However, RA synovial fluids did contain other complexes as shown by the presence of complement consuming activity, C1q binding material and immunoglobulin attaching to conglutinin. It is considered that in RA synovial fluids the complexes containing RF and those activating complement are not necessarily the same whilst in vasculitic sera the complexes containing rheumatoid factor also activate complement.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen C., Elson C. J., Scott D. G., Bacon P. A., Bucknall R. C. IgG antiglobulins in rheumatoid arthritis and other arthritides: relationship with clinical features and other parameters. Ann Rheum Dis. 1981 Apr;40(2):127–131. doi: 10.1136/ard.40.2.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Elson C. J., Scott D. G., Blake D. R., Bacon P. A., Holt P. D. Complement-activating rheumatoid-factor-containing complexes in patients with rheumatoid vasculitis. Ann Rheum Dis. 1983 Apr;42(2):147–150. doi: 10.1136/ard.42.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Grace S. A., Elson C. J. Continuous production of anti-host IgG antibodies contained in circulating IgG-anti-IgG complexes. Immunology. 1982 Oct;47(2):289–296. [PMC free article] [PubMed] [Google Scholar]
  4. Hannestad K. Rheumatoid factors reacting with autologous native gamma-G-globulin and joint fluid gamma-G aggregates. Clin Exp Immunol. 1968 Sep;3(7):671–690. [PMC free article] [PubMed] [Google Scholar]
  5. Harkiss G. D., Brown D. L. Detection of immune complexes by a new assay, the polyethylene glycol precipitation-complement consumption test (PEG-CC). Clin Exp Immunol. 1979 Apr;36(1):117–129. [PMC free article] [PubMed] [Google Scholar]
  6. Jones V. E., Cowley P. J., Allen C., Elson C. J. The isolation of immune complexes containing IgM rheumatoid factor and recovery of IgG rheumatoid factor from the complexes. J Immunol Methods. 1980;37(1):1–14. doi: 10.1016/0022-1759(80)90176-3. [DOI] [PubMed] [Google Scholar]
  7. Jones V. E., Jacoby R. K., Wallington T., Holt P. Immune complexes in early arthritis. L Detection of immune complexes before rheumatoid arthritis is definite. Clin Exp Immunol. 1981 Jun;44(3):512–521. [PMC free article] [PubMed] [Google Scholar]
  8. Male D. K., Roitt I. M. Molecular analysis of complement-fixing rheumatoid synovial fluid immune complexes. Clin Exp Immunol. 1981 Dec;46(3):521–529. [PMC free article] [PubMed] [Google Scholar]
  9. Male D., Roitt I. M., Hay F. C. Analysis of immune complexes in synovial effusions of patients with rheumatoid arthritis. Clin Exp Immunol. 1980 Feb;39(2):297–306. [PMC free article] [PubMed] [Google Scholar]
  10. Nardella F. A., Teller D. C., Mannik M. Studies on the antigenic determinants in the self-association of IgG rheumatoid factor. J Exp Med. 1981 Jul 1;154(1):112–125. doi: 10.1084/jem.154.1.112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nemazee D. A., Sato V. L. Induction of rheumatoid antibodies in the mouse. Regulated production of autoantibody in the secondary humoral response. J Exp Med. 1983 Aug 1;158(2):529–545. doi: 10.1084/jem.158.2.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Perrin L. H., Nydegger U. E., Zubler R. H., Lambert P. H., Miescher P. A. Correlation between levels of breakdown products of C3, C4, and properdin factor B in synovial fluids from patients with rheumatoid arthritis. Arthritis Rheum. 1977 Mar;20(2):647–652. doi: 10.1002/art.1780200202. [DOI] [PubMed] [Google Scholar]
  13. Scott D. G., Bacon P. A., Bothamley J. E., Allen C., Elson C. J., Wallington T. B. Plasma exchange in rheumatoid vasculitis. J Rheumatol. 1981 May-Jun;8(3):433–439. [PubMed] [Google Scholar]
  14. Takahashi M., Takahashi S., Hirose S. Solubilization of antigen-antibody complexes: a new function of complement as a regulator of immune reactions. Prog Allergy. 1980;27:134–166. [PubMed] [Google Scholar]
  15. Theofilopoulos A. N., Burtonboy G., LoSpalluto J. J., Ziff M. IgM rheumatoid factor and low molecular weight IgM. An association with vasculitis. Arthritis Rheum. 1974 May-Jun;17(3):272–284. doi: 10.1002/art.1780170312. [DOI] [PubMed] [Google Scholar]
  16. Weissmann G. Lysosomal mechanisms of tissue injury in arthritis. N Engl J Med. 1972 Jan 20;286(3):141–147. doi: 10.1056/NEJM197201202860307. [DOI] [PubMed] [Google Scholar]
  17. Winchester R. J., Agnello V., Kunkel H. G. Gamma globulin complexes in synovial fluids of patients with rheumatoid arthritis. Partial characterization and relationship to lowered complement levels. Clin Exp Immunol. 1970 May;6(5):689–706. [PMC free article] [PubMed] [Google Scholar]
  18. Zvaifler N. J. Breakdown products of C 3 in human synovial fluids. J Clin Invest. 1969 Aug;48(8):1532–1542. doi: 10.1172/JCI106119. [DOI] [PMC free article] [PubMed] [Google Scholar]

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