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. 1985 Jun;60(3):637–644.

Anti-acetylcholine receptor idiotypes in myasthenia gravis analysed by rabbit anti-sera.

B Lang, A J Roberts, A Vincent, J Newsom-Davis
PMCID: PMC1577199  PMID: 4017289

Abstract

Anti-idiotype sera, raised in rabbits against anti-acetylcholine receptor (AChR) (idiotype) purified from the serum of three myasthenia gravis patients, inhibited binding of homologous idiotype to the AChR by up to 80%. The expression of idiotype in the three individuals changed very little over a period of several years, during which they showed a declining trend in overall anti-AChR antibody. Only one of the four anti-idiotype sera inhibited the binding of anti-AChR from a number of other patients. Our results indicate a consistency of idiotype expression within an individual, and fail to show substantial idiotype sharing between individuals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barkas T., Simpson J. A. Lack of inter-animal cross-reaction of anti-acetylcholine receptor antibodies at the receptor-binding site as demonstrated by heterologous anti-idiotype antisera: implications for immunotherapy of myasthenia gravis. Clin Exp Immunol. 1982 Jan;47(1):119–126. [PMC free article] [PubMed] [Google Scholar]
  2. Delves P. J., Roitt I. M. Idiotypic determinants on human thyroglobulin autoantibodies derived from the serum of Hashimoto patients and EB virus transformed cell lines. Clin Exp Immunol. 1984 Jul;57(1):33–40. [PMC free article] [PubMed] [Google Scholar]
  3. Dwyer D. S., Bradley R. J., Urquhart C. K., Kearney J. F. Naturally occurring anti-idiotypic antibodies in myasthenia gravis patients. Nature. 1983 Feb 17;301(5901):611–614. doi: 10.1038/301611a0. [DOI] [PubMed] [Google Scholar]
  4. Geha R. S., Weinberg R. P. Anti-idiotypic antisera in man. I. Production and immunochemical characterization of anti-idiotypic antisera to human antitetanus antibodies. J Immunol. 1978 Oct;121(4):1518–1523. [PubMed] [Google Scholar]
  5. Lang B., Vincent A., Newsom-Davis J. Purification of anti-acetylcholine receptor antibody from patients with myasthenia gravis. J Immunol Methods. 1982;51(3):371–381. doi: 10.1016/0022-1759(82)90405-7. [DOI] [PubMed] [Google Scholar]
  6. Lefvert A. K. Anti-idiotypic antibodies against the receptor antibodies in myasthenia gravis. Scand J Immunol. 1981;13(5):493–497. doi: 10.1111/j.1365-3083.1981.tb00161.x. [DOI] [PubMed] [Google Scholar]
  7. Lefvert A. K., James R. W., Alliod C., Fulpius B. W. A monoclonal anti-idiotypic antibody against anti-receptor antibodies from myasthenic sera. Eur J Immunol. 1982 Sep;12(9):790–792. doi: 10.1002/eji.1830120917. [DOI] [PubMed] [Google Scholar]
  8. Lennon V. A., Lambert E. H. Monoclonal autoantibodies to acetylcholine receptors: evidence for a dominant idiotype and requirement of complement for pathogenicity. Ann N Y Acad Sci. 1981;377:77–96. doi: 10.1111/j.1749-6632.1981.tb33725.x. [DOI] [PubMed] [Google Scholar]
  9. Lindstrom J., Campbell M., Nave B. Specificities of antibodies to acetylcholine receptors. Muscle Nerve. 1978 Mar-Apr;1(2):140–145. doi: 10.1002/mus.880010206. [DOI] [PubMed] [Google Scholar]
  10. Matsuyama T., Fukumori J., Tanaka H. Evidence of unique idiotypic determinants and similar idiotypic determinants on human antithyroglobulin antibodies. Clin Exp Immunol. 1983 Feb;51(2):381–386. [PMC free article] [PubMed] [Google Scholar]
  11. Newsom-Davis J., Pinching A. J., Vincent A., Wilson S. G. Function of circulating antibody to acetylcholine receptor in myasthenia gravis: investigation by plasma exchange. Neurology. 1978 Mar;28(3):266–272. doi: 10.1212/wnl.28.3.266. [DOI] [PubMed] [Google Scholar]
  12. Schwartz M., Novick D., Givol D., Fuchs S. Induction of anti-idiotypic antibodies by immunisation with syngeneic spleen cells educated with acetylcholine receptor. Nature. 1978 Jun 15;273(5663):543–545. doi: 10.1038/273543a0. [DOI] [PubMed] [Google Scholar]
  13. Vincent A. Immunology of acetylcholine receptors in relation to myasthenia gravis. Physiol Rev. 1980 Jul;60(3):756–824. doi: 10.1152/physrev.1980.60.3.756. [DOI] [PubMed] [Google Scholar]
  14. Vincent A., Newsom-Davis J. Acetylcholine receptor antibody characteristics in myasthenia gravis. I. Patients with generalized myasthenia or disease restricted to ocular muscles. Clin Exp Immunol. 1982 Aug;49(2):257–265. [PMC free article] [PubMed] [Google Scholar]

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