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. 1985 Oct;62(1):13–18.

Human macrophages and T-lymphocyte subsets infiltrating nodules of Onchocerca volvulus.

R M Parkhouse, M Bofill, A Gomez-Priego, G Janossy
PMCID: PMC1577401  PMID: 3905094

Abstract

The nature of the lymphoid infiltrate in nodules of Onchocerca volvulus was assessed using monoclonal antibodies to lymphoid cell surface markers. Although B cells were generally absent, T cells were present, but in variable amounts. The ratio of T4+ (helper phenotype) to T8+ (suppressor-cytotoxic phenotype) was usually in the normal peripheral blood range of about 3, although ratios ranging from 1 to 10 were seen in selected areas of the onchocercoma. The possibility of immunosuppression through dominance of T4+, Leu-8+ cells (suppressor-inducer phenotype) within the T4+ population was also excluded. The T cells did not tend to concentrate in close proximity to the parasite, and there was no general bias in favour of the T suppressor cell phenotype (T8) within the infiltrate. Macrophages and dendritic cells were consistently observed and consisted of three defined cell types in approximately equal proportions: normal, unactivated macrophages (HLA-DR-, acid phosphatase positive), activated macrophages (HLA-DR+, acid phosphatase positive) and cells of dendritic morphology (HLA-DR+, acid phosphatase negative). These results are discussed in relation to immune suppression in filariasis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Askonas B. A. Interference in general immune function by parasite infections; African trypanosomiasis as a model system. Parasitology. 1984 Aug;88(Pt 4):633–638. doi: 10.1017/s0031182000085553. [DOI] [PubMed] [Google Scholar]
  2. Chayen A., Parkhouse R. M. B cell subpopulations in the mouse: analysis with monoclonal antibodies NIM-R2 and NIM-R3. Eur J Immunol. 1982 Sep;12(9):725–732. doi: 10.1002/eji.1830120906. [DOI] [PubMed] [Google Scholar]
  3. Colley D. G., Lewis F. A., Goodgame R. W. Immune responses during human schistosomiasis mansoni. IV. Induction of suppressor cell activity by schistosome antigen preparations and concanavalin A. J Immunol. 1978 Apr;120(4):1225–1232. [PubMed] [Google Scholar]
  4. Gatenby P. A., Kansas G. S., Xian C. Y., Evans R. L., Engleman E. G. Dissection of immunoregulatory subpopulations of T lymphocytes within the helper and suppressor sublineages in man. J Immunol. 1982 Nov;129(5):1997–2000. [PubMed] [Google Scholar]
  5. Gibson D. W., Heggie C., Connor D. H. Clinical and pathologic aspects of onchocerciasis. Pathol Annu. 1980;15(Pt 2):195–240. [PubMed] [Google Scholar]
  6. Ottesen E. A. Modulation of the host response in human schistosomiasis. I. Adherent suppressor cells that inhibit lymphocyte proliferative responses to parasite antigens. J Immunol. 1979 Oct;123(4):1639–1644. [PubMed] [Google Scholar]
  7. Ottesen E. A., Weller P. F., Heck L. Specific cellular immune unresponsiveness in human filariasis. Immunology. 1977 Sep;33(3):413–421. [PMC free article] [PubMed] [Google Scholar]
  8. Piessens W. F., McGreevy P. B., Piessens P. W., McGreevy M., Koiman I., Saroso J. S., Dennis D. T. Immune responses in human infections with Brugia malayi: specific cellular unresponsiveness to filarial antigens. J Clin Invest. 1980 Jan;65(1):172–179. doi: 10.1172/JCI109648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Piessens W. F., Partono F., Hoffman S. L., Ratiwayanto S., Piessens P. W., Palmieri J. R., Koiman I., Dennis D. T., Carney W. P. Antigen-specific suppressor T lymphocytes in human lymphatic filariasis. N Engl J Med. 1982 Jul 15;307(3):144–148. doi: 10.1056/NEJM198207153070302. [DOI] [PubMed] [Google Scholar]
  10. Portaro J. K., Britton S., Ash L. R. Brugia pahangi: depressed mitogen reactivity in filarial infections in the jird, Meriones unguiculatus. Exp Parasitol. 1976 Dec;40(3):438–436. doi: 10.1016/0014-4894(76)90112-0. [DOI] [PubMed] [Google Scholar]
  11. Poulter L. W., Duke O., Hobbs S., Janossy G., Panayi G. Histochemical discrimination of HLA-DR positive cell populations in the normal and arthritic synovial lining. Clin Exp Immunol. 1982 May;48(2):381–388. [PMC free article] [PubMed] [Google Scholar]
  12. Poulter L. W., Seymour G. J., Duke O., Janossy G., Panayi G. Immunohistological analysis of delayed-type hypersensitivity in man. Cell Immunol. 1982 Dec;74(2):358–369. doi: 10.1016/0008-8749(82)90036-3. [DOI] [PubMed] [Google Scholar]
  13. Reinherz E. L., Schlossman S. F. The differentiation and function of human T lymphocytes. Cell. 1980 Apr;19(4):821–827. doi: 10.1016/0092-8674(80)90072-0. [DOI] [PubMed] [Google Scholar]
  14. Selby W. S., Poulter L. W., Hobbs S., Jewell D. P., Janossy G. Heterogeneity of HLA-DR-positive histiocytes in human intestinal lamina propria: a combined histochemical and immunohistological analysis. J Clin Pathol. 1983 Apr;36(4):379–384. doi: 10.1136/jcp.36.4.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Simmonds R. G., Smith W., Marsden H. 3-phenylazo-4-hydroxyphenylisothiocyanates: reagents for efficient haptenation of immunoglobulin and other carrier molecules. J Immunol Methods. 1982 Oct 15;54(1):23–30. doi: 10.1016/0022-1759(82)90109-0. [DOI] [PubMed] [Google Scholar]
  16. Thomas J. A., Janossy G., Chilosi M., Pritchard J., Pincott J. R. Combined immunological and histochemical analysis of skin and lymph node lesions in histiocytosis X. J Clin Pathol. 1982 Mar;35(3):327–337. doi: 10.1136/jcp.35.3.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Todd C. W., Goodgame R. W., Colley D. G. Immune responses during human schistosomiasis mansoni. V. Suppression of schistosome antigen-specific lymphocyte blastogenesis by adherent/phagocytic cells. J Immunol. 1979 Apr;122(4):1440–1446. [PubMed] [Google Scholar]
  18. Unanue E. R. The regulatory role of macrophages in antigenic stimulation. Part Two: symbiotic relationship between lymphocytes and macrophages. Adv Immunol. 1981;31:1–136. doi: 10.1016/s0065-2776(08)60919-0. [DOI] [PubMed] [Google Scholar]
  19. Weller P. F. Cell-mediated immunity in experimental filariasis: lymphocyte reactivity to filarial stage-specific antigens and to B- and T-cell mitogens during acute and chronic infection. Cell Immunol. 1978 May;37(2):369–382. doi: 10.1016/0008-8749(78)90205-8. [DOI] [PubMed] [Google Scholar]

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