Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1985 Oct;62(1):95–103.

Surface glycoproteins as markers of the cellular status of B chronic lymphocytic leukaemia lymphocytes.

V A Brown, S K Smith, A E Dewar, G Stockdill, A H Maddy
PMCID: PMC1577409  PMID: 3877584

Abstract

The phenotype of B CLL cells is investigated with respect to their surface glycoproteins. These glycoproteins are identified by vectorial tritiation followed by 1 and 2 dimensional gel electrophoresis, and by lectin and MoAb binding using immunoprecipitation and flow cytometry. The profiles of the CLL cells are compared with those of normal B cells, prepared from tonsils, and T cells from peripheral blood. The CLL cells show many similarities with T cells, particularly the expression of glycoproteins which bind the MoAbs gpL 115, F10-44-2 and EZB 52, and a complex set of binding sites for Helix pomatia lectin. The significance of these observations in terms of the cellular origins of the leukaemic lymphocytes is discussed.

Full text

PDF
101

Images in this article

97Fig. 1
on p.97
98Fig. 2
on p.98
100Fig. 4
on p.100

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson K. C., Bates M. P., Slaughenhoupt B. L., Pinkus G. S., Schlossman S. F., Nadler L. M. Expression of human B cell-associated antigens on leukemias and lymphomas: a model of human B cell differentiation. Blood. 1984 Jun;63(6):1424–1433. [PubMed] [Google Scholar]
  2. Andersson L. C., Gahmberg C. G., Siimes M. A., Teerenhovi L., Vuopio P. Cell surface glycoprotein analysis: a diagnostic tool in human leukemias. Int J Cancer. 1979 Mar 15;23(3):306–311. doi: 10.1002/ijc.2910230306. [DOI] [PubMed] [Google Scholar]
  3. Andersson L. C., Wasastjerna C., Gahmberg C. G. Different surface glycoprotein patterns on human T-, B- and leukemic-lymphocytes. Int J Cancer. 1976 Jan 15;17(1):40–46. doi: 10.1002/ijc.2910170107. [DOI] [PubMed] [Google Scholar]
  4. Axelsson B., Kimura A., Hammarström S., Wigzell H., Nilsson K., Mellstedt H. Helix pomatia A hemagglutinin: selectivity of binding to lymphocyte surface glycoproteins on T cells and certain B cells. Eur J Immunol. 1978 Nov;8(11):757–764. doi: 10.1002/eji.1830081102. [DOI] [PubMed] [Google Scholar]
  5. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  6. Boumsell L., Coppin H., Pham D., Raynal B., Lemerle J., Dausset J., Bernard A. An antigen shared by a human T cell subset and B cell chronic lymphocytic leukemic cells. Distribution on normal and malignant lymphoid cells. J Exp Med. 1980 Jul 1;152(1):229–234. doi: 10.1084/jem.152.1.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Brown W. R., Williams A. F. Lymphocyte cell surface glycoproteins which bind to soybean and peanut lectins. Immunology. 1982 Aug;46(4):713–726. [PMC free article] [PubMed] [Google Scholar]
  8. Caligaris-Cappio F., Gobbi M., Bofill M., Janossy G. Infrequent normal B lymphocytes express features of B-chronic lymphocytic leukemia. J Exp Med. 1982 Feb 1;155(2):623–628. doi: 10.1084/jem.155.2.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Catovsky D., Cherchi M., Brookss D., Bradely J., Zola H. Heterogeneity of B-cell leukemias demonstrated by the monoclonal antibody FMC7. Blood. 1981 Aug;58(2):406–408. [PubMed] [Google Scholar]
  10. Dalchau R., Kirkley J., Fabre J. W. Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat. Eur J Immunol. 1980 Oct;10(10):737–744. doi: 10.1002/eji.1830101003. [DOI] [PubMed] [Google Scholar]
  11. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  12. Gahmberg C. G., Andersson L. C. Surface glycoproteins of malignant human leukocytes. Biochim Biophys Acta. 1982 Apr 29;651(2-3):65–83. doi: 10.1016/0304-419x(82)90007-5. [DOI] [PubMed] [Google Scholar]
  13. Gordon J., Mellstedt H., Aman P., Biberfeld P., Björkholm M., Klein G. Phenotypes in chronic B-lymphocytic leukemia probed by monoclonal antibodies and immunoglobulin secretion studies: identification of stages of maturation arrest and the relation to clinical findings. Blood. 1983 Oct;62(4):910–917. [PubMed] [Google Scholar]
  14. Greaves M. F., Rao J., Hariri G., Verbi W., Catovsky D., Kung P., Goldstein G. Phenotypic heterogeneity and cellular origins of T cell malignancies. Leuk Res. 1981;5(4-5):281–299. doi: 10.1016/0145-2126(81)90001-1. [DOI] [PubMed] [Google Scholar]
  15. Gupta S., Good R. A. Markers of human lymphocyte subpopulations in primary immunodeficiency and lymphoproliferative disorders. Semin Hematol. 1980 Jan;17(1):1–29. [PubMed] [Google Scholar]
  16. Guy K., Van Heyningen V., Cohen B. B., Deane D. L., Steel C. M. Differential expression and serologically distinct subpopulations of human Ia antigens detected with monoclonal antibodies to Ia alpha and beta chains. Eur J Immunol. 1982 Nov;12(11):942–948. doi: 10.1002/eji.1830121109. [DOI] [PubMed] [Google Scholar]
  17. Guy K., Van Heyningen V., Dewar E., Steel C. M. Enhanced expression of human Ia antigens by chronic lymphocytic leukemia cells following treatment with 12-O-tetradecanoylphorbol-13-acetate. Eur J Immunol. 1983 Feb;13(2):156–159. doi: 10.1002/eji.1830130212. [DOI] [PubMed] [Google Scholar]
  18. Hellström U., Perlmann P., Robertsson E. S., Hammarström S. Receptors for Helix pomatia A haemagglutinin (HP) on a subpopulation of human B cells. Scand J Immunol. 1978 Mar;7(3):191–197. doi: 10.1111/j.1365-3083.1978.tb00443.x. [DOI] [PubMed] [Google Scholar]
  19. Kamoun M., Kadin M. E., Martin P. J., Nettleton J., Hansen J. A. A novel human T cell antigen preferentially expressed on mature T cells and shared by both well and poorly differentiated B cell leukemias and lymphomas. J Immunol. 1981 Sep;127(3):987–991. [PubMed] [Google Scholar]
  20. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  21. McKenzie J. L., Dalchau R., Fabre J. W. Biochemical characterisation and localization in brain of a human brain-leucocyte membrane glycoprotein recognised by a monoclonal antibody. J Neurochem. 1982 Nov;39(5):1461–1466. doi: 10.1111/j.1471-4159.1982.tb12592.x. [DOI] [PubMed] [Google Scholar]
  22. Omary M. B., Trowbridge I. S. Disposition of T200 glycoprotein in the plasma membrane of a murine lymphoma cell line. J Biol Chem. 1980 Feb 25;255(4):1662–1669. [PubMed] [Google Scholar]
  23. Parks D. R., Bryan V. M., Oi V. T., Herzenberg L. A. Antigen-specific identification and cloning of hybridomas with a fluorescence-activated cell sorter. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1962–1966. doi: 10.1073/pnas.76.4.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Pettinelli C. B., McFarlin D. E. Adoptive transfer of experimental allergic encephalomyelitis in SJL/J mice after in vitro activation of lymph node cells by myelin basic protein: requirement for Lyt 1+ 2- T lymphocytes. J Immunol. 1981 Oct;127(4):1420–1423. [PubMed] [Google Scholar]
  25. Remold-O'Donnell E., Kenney D. M., Parkman R., Cairns L., Savage B., Rosen F. S. Characterization of a human lymphocyte surface sialoglycoprotein that is defective in Wiskott-Aldrich syndrome. J Exp Med. 1984 Jun 1;159(6):1705–1723. doi: 10.1084/jem.159.6.1705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Smith S. K., Brown V. A., Dewar A. E., Stockdill G., Cohen B., Maddy A. H. Abnormalities in the expression of the leucocyte-common antigen in chronic lymphocytic leukaemia. Clin Exp Immunol. 1985 Jan;59(1):55–63. [PMC free article] [PubMed] [Google Scholar]
  27. Stashenko P., Nadler L. M., Hardy R., Schlossman S. F. Characterization of a human B lymphocyte-specific antigen. J Immunol. 1980 Oct;125(4):1678–1685. [PubMed] [Google Scholar]
  28. Thompson S., Rennie C. M., Maddy A. H. A re-evaluation of the surface complexity of the intact erythrocyte. Biochim Biophys Acta. 1980 Aug 14;600(3):756–768. doi: 10.1016/0005-2736(80)90478-2. [DOI] [PubMed] [Google Scholar]
  29. Tötterman T. H., Forsbeck K., Nilsson K., Simonsson B., Sundström C., Sällström J. Surface glycoprotein patterns of B type chronic lymphocytic leukaemia cells correlate with the clinical activity of the disease. Scand J Haematol. 1983 Jan;30(1):79–88. doi: 10.1111/j.1600-0609.1983.tb00637.x. [DOI] [PubMed] [Google Scholar]
  30. Wang C. Y., Azzo W., Al-Katib A., Chiorazzi N., Knowles D. M., 2nd Preparation and characterization of monoclonal antibodies recognizing three distinct differentiation antigens (BL1, BL2, BL3) on human B lymphocytes. J Immunol. 1984 Aug;133(2):684–691. [PubMed] [Google Scholar]
  31. Willard-Gallo K. E., Humblet Y., Symann M. Leukocyte membrane proteins in chronic lymphocytic leukemia, as studied by two-dimensional gel electrophoresis. Clin Chem. 1984 Dec;30(12 Pt 1):2069–2077. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES