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. 1985 Dec;62(3):554–560.

Human gamma interferon increases the binding of T lymphocytes to endothelial cells.

C L Yu, D O Haskard, D Cavender, A R Johnson, M Ziff
PMCID: PMC1577468  PMID: 2935340

Abstract

Binding of lymphocytes to human umbilical vein endothelial cells (EC) was quantitated by measuring adhesion of 51Cr labelled lymphocytes to endothelial cell monolayers and rosette formation between lymphocytes and EC in suspension. Mitogen stimulated human peripheral blood mononuclear cell culture supernatants and mixed lymphocyte reaction supernatants enhanced the binding of T lymphocytes to EC monolayers or suspensions preincubated with such supernatants. The active component of these supernatants appeared to be gamma interferon (IFN-gamma) since culture supernatants lost activity after heating at 56 degrees C for 60 min, exposure to pH 2.0 or treatment with anti-IFN-gamma. In addition, purified IFN-gamma increased the binding of T lymphocytes to EC (T-EC). This occurred in a concentration dependent manner when IFN-gamma was preincubated with EC but not with lymphocytes. While the optimum concentration of IFN-gamma was 250 u/ml, a significant enhancement was seen with as little as 10 u/ml. These findings suggest that IFN-gamma may play a part in the emigration of lymphocytes to perivascular chronic inflammatory sites by augmenting the adhesion of lymphocytes to the endothelium of small blood vessels.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson N. D., Anderson A. O., Wyllie R. G. Specialized structure and metabolic activities of high endothelial venules in rat lymphatic tissues. Immunology. 1976 Sep;31(3):455–473. [PMC free article] [PubMed] [Google Scholar]
  2. Cohen M. C., Picciano P. T., Douglas W. J., Yoshida T., Kreutzer D. L., Cohen S. Migration inhibition of endothelial cells by lymphokine-containing supernatants. Science. 1982 Jan 15;215(4530):301–303. doi: 10.1126/science.6797069. [DOI] [PubMed] [Google Scholar]
  3. Freemont A. J., Jones C. J., Bromley M., Andrews P. Changes in vascular endothelium related to lymphocyte collections in diseased synovia. Arthritis Rheum. 1983 Dec;26(12):1427–1433. doi: 10.1002/art.1780261203. [DOI] [PubMed] [Google Scholar]
  4. Freemont A. J., Jones C. J. Endothelial specialization of salivary gland vessels for accelerated lymphocyte transfer in Sjögren's syndrome. J Rheumatol. 1983 Oct;10(5):801–804. [PubMed] [Google Scholar]
  5. Freemont A. J. The small blood vessels in areas of lymphocytic infiltration around malignant neoplasms. Br J Cancer. 1982 Aug;46(2):283–288. doi: 10.1038/bjc.1982.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
  7. GOWANS J. L. The recirculation of lymphocytes from blood to lymph in the rat. J Physiol. 1959 Apr 23;146(1):54–69. doi: 10.1113/jphysiol.1959.sp006177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gifford G. E., Tibor A., Peavy D. L. Interferon production in mixed lymphocyte cell cultures. Infect Immun. 1971 Jan;3(1):164–166. doi: 10.1128/iai.3.1.164-166.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gimbrone M. A., Jr, Cotran R. S., Folkman J. Human vascular endothelial cells in culture. Growth and DNA synthesis. J Cell Biol. 1974 Mar;60(3):673–684. doi: 10.1083/jcb.60.3.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Graham R. C., Jr, Shannon S. L. Peroxidase arthritis. II. Lymphoid cell-endothelial interactions during a developing immunologic inflammatory response. Am J Pathol. 1972 Oct;69(1):7–24. [PMC free article] [PubMed] [Google Scholar]
  11. Greenburg G. B., Hunt T. K. The proliferative response in vitro of vascular endothelial and smooth muscle cells exposed to wound fluids and macrophages. J Cell Physiol. 1978 Dec;97(3 Pt 1):353–360. doi: 10.1002/jcp.1040970310. [DOI] [PubMed] [Google Scholar]
  12. Hendriks H. R., Eestermans I. L. Disappearance and reappearance of high endothelial venules and immigrating lymphocytes in lymph nodes deprived of afferent lymphatic vessels: a possible regulatory role of macrophages in lymphocyte migration. Eur J Immunol. 1983 Aug;13(8):663–669. doi: 10.1002/eji.1830130811. [DOI] [PubMed] [Google Scholar]
  13. Johnson A. R. Human pulmonary endothelial cells in culture. Activities of cells from arteries and cells from veins. J Clin Invest. 1980 Apr;65(4):841–850. doi: 10.1172/JCI109736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MARCHESI V. T., GOWANS J. L. THE MIGRATION OF LYMPHOCYTES THROUGH THE ENDOTHELIUM OF VENULES IN LYMPH NODES: AN ELECTRON MICROSCOPE STUDY. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:283–290. doi: 10.1098/rspb.1964.0002. [DOI] [PubMed] [Google Scholar]
  15. Martin B. M., Gimbrone M. A., Jr, Unanue E. R., Cotran R. S. Stimulation of nonlymphoid mesenchymal cell proliferation by a macrophage-derived growth factor. J Immunol. 1981 Apr;126(4):1510–1515. [PubMed] [Google Scholar]
  16. Pober J. S., Collins T., Gimbrone M. A., Jr, Cotran R. S., Gitlin J. D., Fiers W., Clayberger C., Krensky A. M., Burakoff S. J., Reiss C. S. Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon. Nature. 1983 Oct 20;305(5936):726–729. doi: 10.1038/305726a0. [DOI] [PubMed] [Google Scholar]
  17. Pober J. S., Gimbrone M. A., Jr, Cotran R. S., Reiss C. S., Burakoff S. J., Fiers W., Ault K. A. Ia expression by vascular endothelium is inducible by activated T cells and by human gamma interferon. J Exp Med. 1983 Apr 1;157(4):1339–1353. doi: 10.1084/jem.157.4.1339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Polverini P. J., Cotran P. S., Gimbrone M. A., Jr, Unanue E. R. Activated macrophages induce vascular proliferation. Nature. 1977 Oct 27;269(5631):804–806. doi: 10.1038/269804a0. [DOI] [PubMed] [Google Scholar]
  19. Sidky Y. A., Auerbach R. Lymphocyte-induced angiogenesis: a quantitative and sensitive assay of the graft-vs.-host reaction. J Exp Med. 1975 May 1;141(5):1084–1100. doi: 10.1084/jem.141.5.1084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Smith J. B., McIntosh G. H., Morris B. The migration of cells through chronically inflamed tissues. J Pathol. 1970 Jan;100(1):21–29. doi: 10.1002/path.1711000104. [DOI] [PubMed] [Google Scholar]
  21. Stamper H. B., Jr, Woodruff J. J. An in vitro model of lymphocyte homing. I. Characterization of the interaction between thoracic duct lymphocytes and specialized high-endothelial venules of lymph nodes. J Immunol. 1977 Aug;119(2):772–780. [PubMed] [Google Scholar]
  22. Valle M. J., Jordan G. W., Haahr S., Merigan T. C. Characteristics of immune interferon produced by human lymphocyte cultures compared to other human interferons. J Immunol. 1975 Jul;115(1):230–233. [PubMed] [Google Scholar]
  23. Wheelock E. F. Interferon-like virus-inhibitor induced in human leukocytes by phytohemagglutinin. Science. 1965 Jul 16;149(3681):310–311. [PubMed] [Google Scholar]
  24. Wiener J., Lattes R. G., Spiro D. An electron microscopic study of leukocyte emigration and vascular permeability in tuberculin sensitivity. Am J Pathol. 1967 Mar;50(3):485–521. [PMC free article] [PubMed] [Google Scholar]
  25. Zweiman B., Moskovitz A. R., Lisak R. P. Quantitative assessment of the adherence of normal human blood mononuclear cells to vascular endothelial cell monolayers. Cell Immunol. 1982 Mar 15;68(1):165–172. doi: 10.1016/0008-8749(82)90099-5. [DOI] [PubMed] [Google Scholar]
  26. de Bono D. Endothelium-lymphocyte interactions in vitro. II. Adherence of allergized lymphocytes. Cell Immunol. 1979 Apr;44(1):64–70. doi: 10.1016/0008-8749(79)90028-5. [DOI] [PubMed] [Google Scholar]

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