Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1969 Jan;4(1):17–28.

The potentiating effect of rheumatoid arthritis serum in the immediate phase of nephrotoxic nephritis

J N McCormick, Janet Day, C J Morris, A G S Hill
PMCID: PMC1579046  PMID: 4891888

Abstract

Nephrotoxic nephritis induced in rats was employed as an experimental model to investigate the possible effects of rheumatoid factor on in vivo antigen–antibody reactions. Rats injected simultaneously with rheumatoid arthritis serum and rabbit nephrotoxic globulin showed a three-fold increase in immediate proteinuria compared with rats injected with nephrotoxic globulin alone. This potentiating effect of rheumatoid arthritis serum was evident even when the serum was injected 48 hr after the nephrotoxic globulin and was also apparent to a lesser extent in rats decomplemented by a prior injection of aggregated human IgG. Normal human serum had no effect on the proteinuria produced by a standard dose of nephrotoxic globulin while rheumatoid arthritis serum injected with normal rabbit globulin did not increase urinary protein excretion above baseline levels. In rats injected with rheumatoid arthritis serum and nephrotoxic globulin, human IgM (presumably rheumatoid factor) was detected by immunofluorescence on the glomerular basement membrane along with the nephrotoxic globulin and rat complement and persisted at this site for as long as 42 days after the initial injections. Rheumatoid factor activity was also recovered by elution from glomeruli isolated from rat kidneys 24 hr after the injection of rheumatoid arthritis serum and nephrotoxic globulin.

Full text

PDF
18

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHRISTIAN C. L. Studies of aggregated gamma-globulin. I. Sedimentation, electrophoretic and anticomplementary properties. J Immunol. 1960 Jan;84:112–116. [PubMed] [Google Scholar]
  2. DAVIS J. S., 4th, BOLLET A. J. PROTECTION OF A COMPLEMENT-SENSITIVE ENZYME SYSTEM BY RHEUMATOID FACTOR. J Immunol. 1964 Jan;92:139–144. [PubMed] [Google Scholar]
  3. DUTHIE J. J., BROWN P. E., KNOX J. D., THOMPSON M. Course and prognosis in rheumatoid arthritis. Ann Rheum Dis. 1957 Dec;16(4):411–424. doi: 10.1136/ard.16.4.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EDELMAN G. M., KUNKEL H. G., FRANKLIN E. C. Interaction of the rheumatoid factor with antigen-antibody complexes and aggregated gamma globulin. J Exp Med. 1958 Jul 1;108(1):105–120. doi: 10.1084/jem.108.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HARRIS J., VAUGHAN J. H. Transfusion studies in rheumatoid arthritis. Arthritis Rheum. 1961 Feb;4:47–55. doi: 10.1002/art.1780040105. [DOI] [PubMed] [Google Scholar]
  6. HEIMER R., LEVIN F. M., KAHN M. F. INHIBITION OF COMPLEMENT FIXATION BY HUMAN SERUM. II. THE ACTIVITY OF A GAMMA-LM GLOBULIN AND RHEUMATOID FACTOR IN COMPLEMENT FIXATION REACTIONS. J Immunol. 1963 Dec;91:866–872. [PubMed] [Google Scholar]
  7. KAPLAN M. E., JANDL J. H. The effect of rheumatoid factors and of antigobulins of immune hemolysis in vivo. J Exp Med. 1963 Jan 1;117:105–125. doi: 10.1084/jem.117.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KRAKOWER C. A., GREENSPON S. A. Localization of the nephrotoxic antigen within the isolated renal glomerulus. AMA Arch Pathol. 1951 Jun;51(6):629–639. [PubMed] [Google Scholar]
  9. RESTIFO R. A., LUSSIER A. J., RAWSON A. J., ROCKEY J. H., HOLLANDER J. L. STUDIES ON THE PATHOGENESIS OF RHEUMATOID JOINT INFLAMMATION. 3. THE EXPERIMENTAL PRODUCTION OF ARTHRITIS BY THE INTRA-ARTICULAR INJECTION OF PURIFIED 7S GAMMA GLOBULIN. Ann Intern Med. 1965 Feb;62:285–291. doi: 10.7326/0003-4819-62-2-285. [DOI] [PubMed] [Google Scholar]
  10. RIGGS J. L., SEIWALD R. J., BURCKHALTER J. H., DOWNS C. M., METCALF T. G. Isothiocyanate compounds as fluorescent labeling agents for immune serum. Am J Pathol. 1958 Nov-Dec;34(6):1081–1097. [PMC free article] [PubMed] [Google Scholar]
  11. Romeyn J. A., Bowman D. M. Inhibition of complementary lysis by rheumatoid sera. Nature. 1967 Oct 14;216(5111):180–181. doi: 10.1038/216180a0. [DOI] [PubMed] [Google Scholar]
  12. UNANUE E., DIXON F. J. EXPERIMENTAL GLOMERULONEPHRITIS. IV. PARTICIPATION OF COMPLEMENT IN NEPHROTOXIC NEPHRITIS. J Exp Med. 1964 Jan 1;119:965–982. doi: 10.1084/jem.119.6.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. VAUGHAN J. H. Serum responses in rheumatoid arthritis. Am J Med. 1959 Apr;26(4):596–613. doi: 10.1016/0002-9343(59)90281-5. [DOI] [PubMed] [Google Scholar]
  14. WALLER M., MULLINAX F., TOONE E. C., THURMAN L. A STUDY OF RHEUMATOID FACTOR TITERS IN PATIENTS RECEIVING PLASMA AND GAMMA GLOBULIN. Arthritis Rheum. 1965 Feb;8:50–60. doi: 10.1002/art.1780080106. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES