Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1966 Jan;1(1):85–98.

Erythrocyte survival in normal mice and in mice with autoimmune haemolytic anaemia

E S Lindsey, G W K Donaldson, M F A Woodruff
PMCID: PMC1579164  PMID: 5915102

Abstract

Erythrocyte survival has been studied in a strain of mice which develop autoimmune haemolytic anaemia in adult life (NZB/B1), and also in CBA and C57B1 mice, using one or both of two radioactive isotope labels, 51Cr and 32P.

Erythrocyte survival is customarily expressed as the half-life but this is unsatisfactory for purposes of statistical comparison and we have used instead a parameter `κ' which represents the slope of the line obtained when: log(Counts per unit haemoglobin on day t)/(Counts per unit haemoglobin on day 0) is plotted against time.

Young NZB/B1 mice yield values of κ intermediate between those obtained in CBA and C57B1 mice, and may reasonably be judged to show normal erythrocyte survival. On the other hand, in older NZB/B1 mice which are strongly Coombs positive and show reticulocytosis and anaemia, erythrocyte survival is greatly shortened.

The survival of erythrocytes transfused to NZB/B1 recipients from isogeneic donors was found to be similar to that of the recipient's own erythrocytes, irrespective of whether or not the donor was Coombs positive or showed other evidence of haemolytic activity.

The values of κ obtained in NZB/B1 mice were essentially the same with both labels; in CBA mice, however, a significant difference was observed.

Full text

PDF
89

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON R., HUESTIS R. R., MOTULSKY A. G. Hereditary spherocytosis in the deer mouse. Its similarity to the human disease. Blood. 1960 Apr;15:491–504. [PubMed] [Google Scholar]
  2. BERLIN N. I., LOTZ C. Life span of the red blood cell of the rat following acute hemorrhage. Proc Soc Exp Biol Med. 1951 Dec;78(3):788–790. doi: 10.3181/00379727-78-19220. [DOI] [PubMed] [Google Scholar]
  3. CASSERD F., FINCH C. A., GIBLETT E. R., HOUGHTON B., MOTULSKY A. G. Studies on the pathogenesis of splenic anemia. Blood. 1956 Dec;11(12):1118–1131. [PubMed] [Google Scholar]
  4. CLINE M. J., BERLIN N. I. Red blood cell life span using DFP as a cohort label. Blood. 1962 Jun;19:715–723. [PubMed] [Google Scholar]
  5. CULP N. W., CHAPLIN H., Jr The effects of concentrated eluted anti-red blood cell antibodies on the in vivo survival of normal red blood cells. Blood. 1960 Apr;15:525–533. [PubMed] [Google Scholar]
  6. DONOHUE D. M., MOTULSKY A. G., GIBLETT E. R., PIRZIO-BIROLI G., VIRANUVATTI V., FINCH C. A. The use of chromium as red-cell tag. Br J Haematol. 1955 Jul;1(3):249–263. doi: 10.1111/j.1365-2141.1955.tb05508.x. [DOI] [PubMed] [Google Scholar]
  7. EADIE G. S., SMITH W. W., BROWN I. W., Jr The use of DFP32 as a red cell tag with and without simultaneous tagging with chromium 51 in certain animals in the presence or absence of random destruction. J Gen Physiol. 1960 Mar;43:825–839. doi: 10.1085/jgp.43.4.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. EBAUGH F. G., Jr, EMERSON C. P., ROSS J. F. The use of radioactive chromium 51 as an erythrocyte tagging agent for the determination or red cell survival in vivo. J Clin Invest. 1953 Dec;32(12):1260–1276. doi: 10.1172/JCI102855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GARBY L. Analysis of red-cell survival curves in clinical practice and the use of di-iso-propylfluorophosphonate (DF32P) as a label for red cells in man. Br J Haematol. 1962 Jan;8:15–27. doi: 10.1111/j.1365-2141.1962.tb06490.x. [DOI] [PubMed] [Google Scholar]
  10. HOLMES M. C., BURNET F. M. THE INFLUENCE OF SPLENECTOMY IN NZB MICE. Aust J Exp Biol Med Sci. 1963 Aug;41:SUPPL449–SUPPL455. doi: 10.1038/icb.1963.65. [DOI] [PubMed] [Google Scholar]
  11. JONES N. C., MOLLISON P. L., VEALL N. Removal of incompatible red cells by the spleen. Br J Haematol. 1957 Apr;3(2):125–133. doi: 10.1111/j.1365-2141.1957.tb05779.x. [DOI] [PubMed] [Google Scholar]
  12. LONG G., HOLMES M. C., BURNET F. M. AUTOANTIBODIES PRODUCED AGAINST MOUSE ERYTHROCYTES IN NZB MICE. Aust J Exp Biol Med Sci. 1963 Aug;41:315–322. doi: 10.1038/icb.1963.31. [DOI] [PubMed] [Google Scholar]
  13. Mollison P. L. Survival after Transfusion of Rh-positive Erythrocytes Previously Incubated with Rh Antibody. J Clin Pathol. 1949 May;2(2):109–113. [PMC free article] [PubMed] [Google Scholar]
  14. NECHELES T. F., WEINSTEIN I. M., LEROY G. V. Radioactive sodium chromate for the study of survival of red blood cells. I. The effect of radioactive sodium chromate on red cells. J Lab Clin Med. 1953 Sep;42(3):358–367. [PubMed] [Google Scholar]
  15. OLINER H., SCHWARTZ R., DAMESHEK W. Studies in experimental autoimmune disorders. I. Clinical and laboratory features of autoimmunization (runt disease) in the mouse. Blood. 1961 Jan;17:20–44. [PubMed] [Google Scholar]
  16. RODNAN G. P., EBAUGH F. G., Jr, FOX M. R. S. The life span of the red blood cell and the red blood cell volume in the chicken, pigeon and duck as estimated by the use of Na2Cr51O4, with observations on red cell turnover rate in the mammal, bird and reptile. Blood. 1957 Apr;12(4):355–366. [PubMed] [Google Scholar]
  17. STOHLMAN F., Jr Humoral regulation of erythropoiesis. VII. Shortened survival of erythrocytes produced by erythropoietine or severe anemia. Proc Soc Exp Biol Med. 1961 Aug-Sep;107:884–887. doi: 10.3181/00379727-107-26784. [DOI] [PubMed] [Google Scholar]
  18. STOHLMAN F., Jr, SCHNEIDERMAN M. A. Application of the Cr51 technique to the study of experimental hemolysis in the dog. J Lab Clin Med. 1956 Jan;47(1):72–82. [PubMed] [Google Scholar]
  19. SUTHERLAND D. A., EISENTRAUT A. M., McCALL M. S. The direct Coombs test and reticulocytes. Br J Haematol. 1963 Jan;9:68–76. doi: 10.1111/j.1365-2141.1963.tb05442.x. [DOI] [PubMed] [Google Scholar]
  20. Selwyn J. G., Hackett W. E. Acquired Haemolytic Anaemia: Survival of Transfused Erythrocytes in Patients and Normal Recipients. J Clin Pathol. 1949 May;2(2):114–120. doi: 10.1136/jcp.2.2.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. THOMAS L. CIRCULATING AUTOANTIBODIES AND HUMAN DISEASE. WITH A NOTE ON PRIMARY ATYPICAL PNEUMONIA. N Engl J Med. 1964 May 28;270:1157–1159. doi: 10.1056/NEJM196405282702204. [DOI] [PubMed] [Google Scholar]
  22. VAN PUTTEN L. M. The life span of red cells in the rat and the mouse as determined by labeling with DFP32 in vivo. Blood. 1958 Aug;13(8):789–794. [PubMed] [Google Scholar]
  23. WASSERMAN M., RUBIN H. TRUE RED BLOOD CELL SURVIVAL WITH RADIOCHROMIUM BY CORRECTING FOR PERCENTAGE OF ACTIVITY NOT BOUND TO HEMOGLOBIN. DEMONSTRATION OF TWO ERYTHROCYTE POPULATIONS IN NORMALS AND IN THALASSEMIA MINOR. ERYTHROPOIESIS AND THE THALASSEMIA TRAIT. Ann N Y Acad Sci. 1964 Oct 7;119:586–606. doi: 10.1111/j.1749-6632.1965.tb54059.x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES