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. 1997 Dec;115(4):1533–1540. doi: 10.1104/pp.115.4.1533

Fluence and wavelength requirements for Arabidopsis CAB gene induction by different phytochromes.

F Hamazato 1, T Shinomura 1, H Hanzawa 1, J Chory 1, M Furuya 1
PMCID: PMC158619  PMID: 9414562

Abstract

The roles of different phytochromes have been investigated in the photoinduction of several chlorophyll a/b-binding protein genes (CAB) of Arabidopsis thaliana. Etiolated seedlings of the wild type, a phytochrome A (PhyA) null mutant (phyA), a phytochrome B (PhyB) null mutant (phyB), and phyA/phyB double mutant were exposed to monochromatic light to address the questions of the fluence and wavelength requirements for CAB induction by different phytochromes. In the wild type and the phyB mutant, PhyA photoirreversibly induced CAB expression upon irradiation with very-low-fluence light of 350 to 750 nm. In contrast, using the phyA mutant, PhyB photoreversibly induced CAB expression with low-fluence red light. The threshold fluences of red light for PhyA- and PhyB-specific induction were about 10 nmol m-2 and 10 mumol m-2, respectively. In addition, CAB expression was photoreversibly induced with low-fluence red light in the phyA/phyB double mutant, revealing that another phytochrome(s) (PhyX) regulated CAB expression in a manner similar to PhyB. These data suggest that plants utilize different phytochromes to perceive light of varying wave-lengths and fluence, and begin to explain how plants respond so exquisitely to changing light in their environment.

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Selected References

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  1. Apel K. Phytochrome-induced appearance of mRNA activity for the apoprotein of the light-harvesting chlorophyll a/b protein of barley (Hordeum vulgare). Eur J Biochem. 1979 Jun;97(1):183–188. doi: 10.1111/j.1432-1033.1979.tb13101.x. [DOI] [PubMed] [Google Scholar]
  2. Bagnall D. J., King R. W., Whitelam G. C., Boylan M. T., Wagner D., Quail P. H. Flowering responses to altered expression of phytochrome in mutants and transgenic lines of Arabidopsis thaliana (L.) Heynh. Plant Physiol. 1995 Aug;108(4):1495–1503. doi: 10.1104/pp.108.4.1495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Botto J. F., Sanchez R. A., Whitelam G. C., Casal J. J. Phytochrome A Mediates the Promotion of Seed Germination by Very Low Fluences of Light and Canopy Shade Light in Arabidopsis. Plant Physiol. 1996 Feb;110(2):439–444. doi: 10.1104/pp.110.2.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brusslan J. A., Tobin E. M. Light-independent developmental regulation of cab gene expression in Arabidopsis thaliana seedlings. Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7791–7795. doi: 10.1073/pnas.89.16.7791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carabelli M., Morelli G., Whitelam G., Ruberti I. Twilight-zone and canopy shade induction of the Athb-2 homeobox gene in green plants. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3530–3535. doi: 10.1073/pnas.93.8.3530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Devlin P. F., Halliday K. J., Harberd N. P., Whitelam G. C. The rosette habit of Arabidopsis thaliana is dependent upon phytochrome action: novel phytochromes control internode elongation and flowering time. Plant J. 1996 Dec;10(6):1127–1134. doi: 10.1046/j.1365-313x.1996.10061127.x. [DOI] [PubMed] [Google Scholar]
  7. Karlin-Neumann G. A., Sun L., Tobin E. M. Expression of Light-Harvesting Chlorophyll a/b-Protein Genes Is Phytochrome-Regulated in Etiolated Arabidopsis thaliana Seedlings. Plant Physiol. 1988 Dec;88(4):1323–1331. doi: 10.1104/pp.88.4.1323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaufman L. S., Thompson W. F., Briggs W. R. Different Red Light Requirements for Phytochrome-Induced Accumulation of cab RNA and rbcS RNA. Science. 1984 Dec 21;226(4681):1447–1449. doi: 10.1126/science.226.4681.1447. [DOI] [PubMed] [Google Scholar]
  9. Kaufman L. S. Transduction of Blue-Light Signals. Plant Physiol. 1993 Jun;102(2):333–337. doi: 10.1104/pp.102.2.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leutwiler L. S., Meyerowitz E. M., Tobin E. M. Structure and expression of three light-harvesting chlorophyll a/b-binding protein genes in Arabidopsis thaliana. Nucleic Acids Res. 1986 May 27;14(10):4051–4064. doi: 10.1093/nar/14.10.4051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McGrath J. M., Terzaghi W. B., Sridhar P., Cashmore A. R., Pichersky E. Sequence of the fourth and fifth Photosystem II type I chlorophyll a/b-binding protein genes of Arabidopsis thaliana and evidence for the presence of a full complement of the extended CAB gene family. Plant Mol Biol. 1992 Aug;19(5):725–733. doi: 10.1007/BF00027069. [DOI] [PubMed] [Google Scholar]
  12. Millar A. J., Kay S. A. Integration of circadian and phototransduction pathways in the network controlling CAB gene transcription in Arabidopsis. Proc Natl Acad Sci U S A. 1996 Dec 24;93(26):15491–15496. doi: 10.1073/pnas.93.26.15491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nagatani A., Reed J. W., Chory J. Isolation and Initial Characterization of Arabidopsis Mutants That Are Deficient in Phytochrome A. Plant Physiol. 1993 May;102(1):269–277. doi: 10.1104/pp.102.1.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oelmüller R., Kendrick R. E., Briggs W. R. Blue-light mediated accumulation of nuclear-encoded transcripts coding for proteins of the thylakoid membrane is absent in the phytochrome-deficient aurea mutant of tomato. Plant Mol Biol. 1989 Aug;13(2):223–232. doi: 10.1007/BF00016140. [DOI] [PubMed] [Google Scholar]
  15. Quail P. H., Boylan M. T., Parks B. M., Short T. W., Xu Y., Wagner D. Phytochromes: photosensory perception and signal transduction. Science. 1995 May 5;268(5211):675–680. doi: 10.1126/science.7732376. [DOI] [PubMed] [Google Scholar]
  16. Reed J. W., Nagatani A., Elich T. D., Fagan M., Chory J. Phytochrome A and Phytochrome B Have Overlapping but Distinct Functions in Arabidopsis Development. Plant Physiol. 1994 Apr;104(4):1139–1149. doi: 10.1104/pp.104.4.1139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reed J. W., Nagpal P., Poole D. S., Furuya M., Chory J. Mutations in the gene for the red/far-red light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development. Plant Cell. 1993 Feb;5(2):147–157. doi: 10.1105/tpc.5.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shinomura T., Nagatani A., Hanzawa H., Kubota M., Watanabe M., Furuya M. Action spectra for phytochrome A- and B-specific photoinduction of seed germination in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):8129–8133. doi: 10.1073/pnas.93.15.8129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shirzadegan M., Christie P., Seemann J. R. An efficient method for isolation of RNA from tissue cultured plant cells. Nucleic Acids Res. 1991 Nov 11;19(21):6055–6055. doi: 10.1093/nar/19.21.6055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Silverthorne J., Tobin E. M. Demonstration of transcriptional regulation of specific genes by phytochrome action. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1112–1116. doi: 10.1073/pnas.81.4.1112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Somers D. E., Sharrock R. A., Tepperman J. M., Quail P. H. The hy3 Long Hypocotyl Mutant of Arabidopsis Is Deficient in Phytochrome B. Plant Cell. 1991 Dec;3(12):1263–1274. doi: 10.1105/tpc.3.12.1263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wehmeyer B., Cashmore A. R., Schäfer E. Photocontrol of the Expression of Genes Encoding Chlorophyll a/b Binding Proteins and Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase in Etiolated Seedlings of Lycopersicon esculentum (L.) and Nicotiana tabacum (L.). Plant Physiol. 1990 Jul;93(3):990–997. doi: 10.1104/pp.93.3.990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. White M. J., Kaufman L. S., Horwitz B. A., Briggs W. R., Thompson W. F. Individual Members of the Cab Gene Family Differ Widely in Fluence Response. Plant Physiol. 1995 Jan;107(1):161–165. doi: 10.1104/pp.107.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Zhang H., Hanley S., Goodman H. M. Isolation, Characterization, and Chromosomal Location of a New cab Gene from Arabidopsis thaliana. Plant Physiol. 1991 Aug;96(4):1387–1388. doi: 10.1104/pp.96.4.1387. [DOI] [PMC free article] [PubMed] [Google Scholar]

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