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. 1983 Jan;58(1):34–38. doi: 10.1136/adc.58.1.34

Identification of lymphocyte subsets in the newborn using a variety of monoclonal antibodies.

R M Thomas, D C Linch
PMCID: PMC1628156  PMID: 6219626

Abstract

Using a small sample of peripheral venous blood, the normal range for lymphocyte subpopulations (T-cell, B-cell, T-suppressor cell, and T-helper cell) was defined in non-infected preterm and term infants. Lymphocyte subsets were identified using a variety of monoclonal antisera, and analysis was performed using a fluorescent activated cell sorter. Such methods allow an objective assessment of absolute numbers of cells per unit volume of whole blood. There was no significant difference in absolute numbers of lymphocyte subsets between term and preterm appropriate for gestational age (AGA) infants. Infants who were small for gestational age (SGA) had a significant deficiency in absolute numbers of total T-cells, helper and inducer T-lymphocytes, and B-cells compared with both term and preterm AGA infants. All newborn infants (term and preterm; AGA and SGA) had a highly significant increase in absolute numbers of both helper and suppressor T-lymphocytes compared with normal adults.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beverley P. C., Linch D., Delia D. Isolation of human haematopoietic progenitor cells using monoclonal antibodies. Nature. 1980 Sep 25;287(5780):332–333. doi: 10.1038/287332a0. [DOI] [PubMed] [Google Scholar]
  2. Bonforte R. J., Topilsky M., Siltzbach L. E., Glade P. R. Phytohemagglutinin skin test: a possible in vivo measure of cell-mediated immunity. J Pediatr. 1972 Oct;81(4):775–780. doi: 10.1016/s0022-3476(72)80101-x. [DOI] [PubMed] [Google Scholar]
  3. Campbell A. C., Waller C., Wood J., Aynsley-Green A., Yu V. Lymphocyte subpopulations in the blood of newborn infants. Clin Exp Immunol. 1974 Dec;18(4):469–482. [PMC free article] [PubMed] [Google Scholar]
  4. Chandra R. K. Fetal malnutrition and postnatal immunocompetence. Am J Dis Child. 1975 Apr;129(4):450–454. doi: 10.1001/archpedi.1975.02120410038013. [DOI] [PubMed] [Google Scholar]
  5. Christiansen J. S., Osther K., Peitersen B., Bach-Mortensen N. B, T and null lymphocytes in newborn infants and their mothers. Acta Paediatr Scand. 1976 Jul;65(4):425–428. doi: 10.1111/j.1651-2227.1976.tb04909.x. [DOI] [PubMed] [Google Scholar]
  6. Evans R. L., Wall D. W., Platsoucas C. D., Siegal F. P., Fikrig S. M., Testa C. M., Good R. A. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981 Jan;78(1):544–548. doi: 10.1073/pnas.78.1.544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ferguson A. C., Lawlor G. J., Jr, Neumann C. G., Oh W., Stiehm E. R. Decreased rosette-forming lymphocytes in malnutrition and intrauterine growth retardation. J Pediatr. 1974 Nov;85(5):717–723. doi: 10.1016/s0022-3476(74)80527-5. [DOI] [PubMed] [Google Scholar]
  8. Ferguson A. C. Prolonged impairment of cellular immunity in children with intrauterine growth retardation. J Pediatr. 1978 Jul;93(1):52–56. doi: 10.1016/s0022-3476(78)80599-x. [DOI] [PubMed] [Google Scholar]
  9. Fleisher T. A., Luckasen J. R., Sabad A., Gehrtz R. C., Kersey J. H. T and B lymphocyte subpopulations in children. Pediatrics. 1975 Feb;55(2):162–165. [PubMed] [Google Scholar]
  10. Hallberg A., Hallberg T. Evolution of three lymphocyte markers in newborn, preterm infants. Int Arch Allergy Appl Immunol. 1977;55(1-6):102–111. doi: 10.1159/000231916. [DOI] [PubMed] [Google Scholar]
  11. Hayward A. R., Merrill D. Requirement for OKT8+ suppressor cell proliferation for suppression by human newborn T cells. Clin Exp Immunol. 1981 Sep;45(3):468–474. [PMC free article] [PubMed] [Google Scholar]
  12. Ugazio A. G., Marcioni A. F., Astaldi A., Jr, Burgio G. R. Peripheral blood B lymphocytes in infancy and childhood. Acta Paediatr Scand. 1974 Mar;63(2):205–208. doi: 10.1111/j.1651-2227.1974.tb04785.x. [DOI] [PubMed] [Google Scholar]
  13. Wara D. W., Barrett D. J. Cell-mediated immunity in the newborn: clinical aspects. Pediatrics. 1979 Nov;64(5 Pt 2 Suppl):822–828. [PubMed] [Google Scholar]
  14. Yeung C. Y., Hobbs J. R. Serum-gamma-G-globulin levels in normal premature, post-mature, and "small-for-dates" newborn babies. Lancet. 1968 Jun 1;1(7553):1167–1170. doi: 10.1016/s0140-6736(68)91865-5. [DOI] [PubMed] [Google Scholar]
  15. Yu V. Y., Waller C. A., Maclennan I. C., Baum J. D. Lymphocyte reactivity in pregnant women and newborn infants. Br Med J. 1975 Feb 22;1(5955):428–432. doi: 10.1136/bmj.1.5955.428. [DOI] [PMC free article] [PubMed] [Google Scholar]

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