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. 1972 Jan;44(1):10–16. doi: 10.1111/j.1476-5381.1972.tb07233.x

Resistance of noradrenaline in blood vessels to depletion by 6-hydroxydopamine or immunosympathectomy

B A Berkowitz, S Spector, J H Tarver
PMCID: PMC1665696  PMID: 4401306

Abstract

1. The degree of the decrease in the noradrenaline concentrations caused by 6-hydroxydopamine or immunosympathectomy was different in different areas of the cardiovascular system.

2. In rats or guinea-pigs 6-hydroxydopamine depleted the noradrenaline content of the heart by 90%, of the mesenteric vein by 80% and of the mesenteric artery and aorta by 30-60%. Immunosympathectomy elicited a 70% reduction in the cardiac noradrenaline but only a 50% reduction in the noradrenaline of the blood vessels of the rat.

3. The tyrosine hydroxylase activity of the heart, blood vessels, or adrenal glands was not significantly altered 2 weeks after 6-hydroxydopamine. Nor was the monoamine oxidase activity in heart or blood vessels changed.

4. The inconsistent ability of both 6-hydroxydopamine and immunosympathectomy to abolish experimental hypertension may be due to the partial persistence of noradrenaline and functional sympathetic nervous system activity in the blood vessels.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANTON A. H., SAYRE D. F. A study of the factors affecting the aluminum oxide-trihydroxyindole procedure for the analysis of catecholamines. J Pharmacol Exp Ther. 1962 Dec;138:360–375. [PubMed] [Google Scholar]
  2. Ayitey-Smith E., Varma D. R. An assessment of the role of the sympathetic nervous system in experimental hypertension using normal and immunosympathectomized rats. Br J Pharmacol. 1970 Oct;40(2):175–185. doi: 10.1111/j.1476-5381.1970.tb09911.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berkowitz B. A., Spector S., Brossi A., Focella A., Teitel S. Preparation and biological properties of (-)- and (+)-6-hydroxydopa. Experientia. 1970 Sep 26;26(9):982–983. doi: 10.1007/BF02114144. [DOI] [PubMed] [Google Scholar]
  4. Berkowitz B. A., Tarver J. H., Spector S. Norepinephrine in blood vessels: concentration, binding, uptake and depletion. J Pharmacol Exp Ther. 1971 Apr;177(1):119–126. [PubMed] [Google Scholar]
  5. Bigelow L. B., Dairman W., Weil-Malherbe H., Udenfriend S. Increased synthesis of catecholamines and their metabolites following the administration of phenoxybenzamine. Mol Pharmacol. 1969 Nov;5(6):565–571. [PubMed] [Google Scholar]
  6. CARPI A., OLIVERIO A. URINARY EXCRETION OF CATECHOL AMINES IN THE IMMUNOSYMPATHECTOMIZED RAT--BALANCE PHENOMENA BETWEEN THE ADRENERGIC AND THE NORADRENERGIC SYSTEM. Int J Neuropharmacol. 1964 Sep;3:427–431. doi: 10.1016/0028-3908(64)90073-5. [DOI] [PubMed] [Google Scholar]
  7. Ceasar P. M., Ruthven C. R., Sandler M. Catecholamine and 5-hydroxyindole metabolism in immunosympathectomized rats. Br J Pharmacol. 1969 May;36(1):70–78. doi: 10.1111/j.1476-5381.1969.tb08304.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Clark D. W. Effects of immunosympathectomy on development of high blood pressure in genetically hypertensive rats. Circ Res. 1971 Mar;28(3):330–336. doi: 10.1161/01.res.28.3.330. [DOI] [PubMed] [Google Scholar]
  9. De la Lande I. S., Frewin D., Waterson J. G. The influence of sympathetic innervation on vascular sensitivity to noradrenaline. Br J Pharmacol Chemother. 1967 Sep;31(1):82–93. doi: 10.1111/j.1476-5381.1967.tb01979.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. De la Lande I. S., Hill B. D., Jellett L. B., McNeil J. M. The role of monoamine oxidase in the response of the isolated central artery of the rabbit ear to tyramine. Br J Pharmacol. 1970 Oct;40(2):249–256. doi: 10.1111/j.1476-5381.1970.tb09918.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Finch L., Leach G. D. A comparison of the effects of 6-hydroxydopamine immunosympathectomy and reserpine on the cardiovascular reactivity in the rat. J Pharm Pharmacol. 1970 May;22(5):354–360. doi: 10.1111/j.2042-7158.1970.tb08537.x. [DOI] [PubMed] [Google Scholar]
  12. Finch L., Leach G. D. The contribution of the sympathetic nervous system to the development and maintenance of experimental hypertension in the rat. Br J Pharmacol. 1970 Jun;39(2):317–324. doi: 10.1111/j.1476-5381.1970.tb12895.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Finch L., Leach G. F. Does the adrenal medulla contribute to the maintenance of experimental hypertension? Eur J Pharmacol. 1970;11(3):388–391. doi: 10.1016/0014-2999(70)90016-6. [DOI] [PubMed] [Google Scholar]
  14. Goldman H., Jacobowitz D. Correlation of norepinephrine content with observations of adrenergic nerves after a single dose of 6-hydroxydopamine in the rat. J Pharmacol Exp Ther. 1971 Jan;176(1):119–133. [PubMed] [Google Scholar]
  15. HAMBERGER B., LEVI-MONTALCINI R., NORBERG K. A., SJOEQVIST F. MONOAMINES IN IMMUNOSYMPATHECTOMIZED RATS. Int J Neuropharmacol. 1965 Apr;4:91–95. doi: 10.1016/0028-3908(65)90032-8. [DOI] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Levi-Montalcini R., Angeletti P. U. Second symposium on catecholamines. Modification of sympathetic function. Immunosympathectomy. Pharmacol Rev. 1966 Mar;18(1):619–628. [PubMed] [Google Scholar]
  18. Lowe M. C., Horita A. Stability of cardiac monoamine oxidase activity after chemical sympathectomy with 6-hydroxydopamine. Nature. 1970 Oct 10;228(5267):175–176. doi: 10.1038/228175a0. [DOI] [PubMed] [Google Scholar]
  19. NAGATSU T., LEVITT M., UDENFRIEND S. A RAPID AND SIMPLE RADIOASSAY FOR TYROSINE HYDROXYLASE ACTIVITY. Anal Biochem. 1964 Sep;9:122–126. doi: 10.1016/0003-2697(64)90092-2. [DOI] [PubMed] [Google Scholar]
  20. Ong H. H., Creveling C. R., Daly J. W. The synthesis of 2,4,5-trihydroxyphenylalanine (6-hydroxydopa). A centrally active norepinephrine-depleting agent. J Med Chem. 1969 May;12(3):458–462. doi: 10.1021/jm00303a028. [DOI] [PubMed] [Google Scholar]
  21. SHORE P. A., OLIN J. S. Identification and chemical assay of norepinephrine in brain and other tissues. J Pharmacol Exp Ther. 1958 Mar;122(3):295–300. [PubMed] [Google Scholar]
  22. STONE C. A., STAVORSKI J. M., LUDDEN C. T., WENGER H. C., ROSS C. A., TOTARO J. A., PORTER C. C. COMPARISON OF SOME PHARMACOLOGIC EFFECTS OF CERTAIN 6-SUBSTITUTED DOPAMINE DERIVATIVES WITH RESERPINE, GUANETHIDINE AND METARAMINOL. J Pharmacol Exp Ther. 1963 Nov;142:147–156. [PubMed] [Google Scholar]
  23. Siggins G. R., Bloom F. E. Cytochemical and physiological effects of 6-hydroxydopamine on periarteriolar nerves of frogs. Circ Res. 1970 Jul;27(1):23–38. doi: 10.1161/01.res.27.1.23. [DOI] [PubMed] [Google Scholar]
  24. Tarver J., Berkowitz B., Spector S. Alterations in tyrosine hydroxylase and monoamine oxidase activity in blood vessels. Nat New Biol. 1971 Jun 23;231(25):252–253. doi: 10.1038/newbio231252a0. [DOI] [PubMed] [Google Scholar]
  25. Thoenen H., Mueller R. A., Axelrod J. Increased tyrosine hydroxylase activity after drug-induced alteration of sympathetic transmission. Nature. 1969 Mar 29;221(5187):1264–1264. doi: 10.1038/2211264a0. [DOI] [PubMed] [Google Scholar]
  26. Thoenen H., Mueller R. A., Axelrod J. Trans-synaptic induction of adrenal tyrosine hydroxylase. J Pharmacol Exp Ther. 1969 Oct;169(2):249–254. [PubMed] [Google Scholar]
  27. Thoenen H., Tranzer J. P. Chemical sympathectomy by selective destruction of adrenergic nerve endings with 6-Hydroxydopamine. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1968;261(3):271–288. doi: 10.1007/BF00536990. [DOI] [PubMed] [Google Scholar]
  28. Varma D. R. Antihypertensive effect of methyldopa in metacorticoid immunosympathectomized rats. J Pharm Pharmacol. 1967 Jan;19(1):61–62. doi: 10.1111/j.2042-7158.1967.tb07998.x. [DOI] [PubMed] [Google Scholar]
  29. WURTMAN R. J., AXELROD J. A SENSITIVE AND SPECIFIC ASSAY FOR THE ESTIMATION OF MONOAMINE OXIDASE. Biochem Pharmacol. 1963 Dec;12:1439–1441. doi: 10.1016/0006-2952(63)90215-6. [DOI] [PubMed] [Google Scholar]

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