Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1971 Dec;43(4):784–793. doi: 10.1111/j.1476-5381.1971.tb07214.x

Kallikrein, trypsin-like proteases and amylase in mammalian submaxillary glands

K D Bhoola, Gundula Dorey
PMCID: PMC1665706  PMID: 5152028

Abstract

1. The tissue concentration of kallikrein, trypsin-like proteases and amylase has been determined in the submaxillary glands of the cat, dog, rabbit, rat, hamster and guinea-pig.

2. Submaxillary gland extracts of the six species showed a wide variation in kallikrein content. The esterase activity in the extracts, however, did not correlate with the kininogenase activity of kallikrein. Both activities in each of the six species were affected by proteinase inhibitors but not in the same way.

3. Evidence is presented which indicates that trypsin-like proteases similar to those described in the rat, also occur in the hamster and cat submaxillary glands.

4. An even greater spread of amylase activity was observed between the species.

5. The assumption that mammalian submaxillary kallikreins are identical proteins is questioned.

Full text

PDF
787

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhoola K. D. Comparative study of the subcellular distribution of submaxillary kallikrein. Biochem Pharmacol. 1969 May;18(5):1252–1254. doi: 10.1016/0006-2952(69)90134-8. [DOI] [PubMed] [Google Scholar]
  2. Bhoola K. D., Heap P. F. Properties of kallikrein-containing granules isolated from the submaxillary gland of the guinea-pig. J Physiol. 1970 Sep;210(2):421–432. doi: 10.1113/jphysiol.1970.sp009218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bhoola K. D. Intracellular distribution of submaxillary kallikrein. J Physiol. 1968 May;196(2):431–445. doi: 10.1113/jphysiol.1968.sp008516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bhoola K. D., Ogle C. W. The subcellular localization of kallikrein, amylase and acetylcholine in the submaxillary gland of the guinea-pig. J Physiol. 1966 Jun;184(3):663–672. doi: 10.1113/jphysiol.1966.sp007939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chiang T. S., Erdös E. G., Miwa I., Tague L. L., Coalson J. J. Isolation from a salivary gland of granules containing renin and kallikrein. Circ Res. 1968 Oct;23(4):507–517. doi: 10.1161/01.res.23.4.507. [DOI] [PubMed] [Google Scholar]
  6. ERLANGER B. F., KOKOWSKY N., COHEN W. The preparation and properties of two new chromogenic substrates of trypsin. Arch Biochem Biophys. 1961 Nov;95:271–278. doi: 10.1016/0003-9861(61)90145-x. [DOI] [PubMed] [Google Scholar]
  7. Ekfors T. O., Hopsu-Havu V. K., Malniharju T. Increases vascular permeability caused by the trypsin-like enzymes purified from rat submandibular gland. Acta Physiol Scand. 1969 Jan-Feb;75(1):157–160. doi: 10.1111/j.1748-1716.1969.tb04367.x. [DOI] [PubMed] [Google Scholar]
  8. Ekfors T. O., Malmiharju T., Riekkinen P. J., Hopsu-havu V. K. The depressor activity of trypsin-like enzymes purified from rat submandibular gland. Biochem Pharmacol. 1967 Aug;16(8):1634–1636. doi: 10.1016/0006-2952(67)90143-8. [DOI] [PubMed] [Google Scholar]
  9. Ekfors T. O., Riekkinen P. J., Malmiharju T., Hopsu-Havu V. K. Four isozymic forms of a peptidase resembling kallikrein purified from the rat submandibular gland. Hoppe Seylers Z Physiol Chem. 1967 Jan;348(1):111–118. doi: 10.1515/bchm2.1967.348.1.111. [DOI] [PubMed] [Google Scholar]
  10. Erdös E. G., Tague L. L., Miwa I. Kallikrein in granules of the submaxillary gland. Biochem Pharmacol. 1968 May;17(5):667–674. doi: 10.1016/0006-2952(68)90003-8. [DOI] [PubMed] [Google Scholar]
  11. Ferreira S. H., Vane J. R. The detection and estimation of bradykinin in the circulating blood. Br J Pharmacol Chemother. 1967 Mar;29(3):367–377. doi: 10.1111/j.1476-5381.1967.tb01968.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fiedler F., Müller B., Werle E. Charakterisierung verschiedener Schweinekallikreine mittels Diisopropylfluorophosphat. Hoppe Seylers Z Physiol Chem. 1970 Aug;351(8):1002–1006. [PubMed] [Google Scholar]
  13. Fiedler F., Müller B., Werle E. The inhibition of porcine pancreas kallikrein by di-isopropyl-fluorophosphate. Kinetics, stoichiometry, and nature of the group phosphorylated. Eur J Biochem. 1969 Oct;10(3):419–425. doi: 10.1111/j.1432-1033.1969.tb00706.x. [DOI] [PubMed] [Google Scholar]
  14. HABERMANN E. [Differentiation between kallikrein and kallikrein elements by means of antiserum and diisopropylfluorophosphate]. Hoppe Seylers Z Physiol Chem. 1962 Jun 20;328:24–30. doi: 10.1515/bchm2.1962.328.1.24. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Riekkinen P. J., Ekfors T. O., Hollmén T., Hopsu-Havu V. K. Purification and physical characteristics of a trypsin-like protease from the rat submandibular gland. Enzymologia. 1967 Feb 28;32(2):97–109. [PubMed] [Google Scholar]
  17. Riekkinen P. J., Ekfors T. O., Hopsu-Havu V. K. Substrate specificity and modifier characteristics of an alkaline protease from the rat submandibular gland: glandulain. Enzymologia. 1967 Feb 28;32(2):110–127. [PubMed] [Google Scholar]
  18. Riekkinen P. J., Ekfors T. O., Hopsu V. K. Purification and characteristics of an alkaline protease from rat-submandibular gland. Biochim Biophys Acta. 1966 Jun 15;118(3):604–620. doi: 10.1016/s0926-6593(66)80101-7. [DOI] [PubMed] [Google Scholar]
  19. Riekkinen P. J., Niemi M. Androgen-dependent salivary gland protease in the rat. Endocrinology. 1968 Dec;83(6):1224–1231. doi: 10.1210/endo-83-6-1224. [DOI] [PubMed] [Google Scholar]
  20. SCHRAMM M., DANON D. The mechanism of enzyme secretion by the cell. I. Storage of amylase in the zymogen granules of the rat-parotis gland. Biochim Biophys Acta. 1961 Jun 10;50:102–112. doi: 10.1016/0006-3002(61)91065-4. [DOI] [PubMed] [Google Scholar]
  21. SHACKLEFORD J. M., KLAPPER E. C. Structure and carbohydrate histochemistry of mammalian salivary glands. Am J Anat. 1962 Jul;111:25–47. doi: 10.1002/aja.1001110104. [DOI] [PubMed] [Google Scholar]
  22. Schachter M. Kallikreins and kinins. Physiol Rev. 1969 Jul;49(3):509–547. doi: 10.1152/physrev.1969.49.3.509. [DOI] [PubMed] [Google Scholar]
  23. Shackleford J. M., Wilborn W. H. Structural and histochemical diversity in mammalian salivary glands. Ala J Med Sci. 1968 Apr;5(2):180–203. [PubMed] [Google Scholar]
  24. TRAUTSCHOLD I., WERLE E. [Spectrophotometric determination of kallikrein and its inactivators]. Hoppe Seylers Z Physiol Chem. 1961 Jun 30;325:48–59. doi: 10.1515/bchm2.1961.325.1.48. [DOI] [PubMed] [Google Scholar]
  25. WERLE E., KAUFMANN-BOETSCH B. [On the esterase effects of kallikrein and trypsin and their inhibition by kallikrein-and trypsin inhibitors]. Hoppe Seylers Z Physiol Chem. 1960;319:52–63. doi: 10.1515/bchm2.1960.319.1.52. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES