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. 1972 Sep;46(1):101–115. doi: 10.1111/j.1476-5381.1972.tb06853.x

Release of monoamines from the striatum and hypothalamus

Effect of γ-hydroxybutyrate

G Bustos, R H Roth
PMCID: PMC1666124  PMID: 5084815

Abstract

1. A simple in vitro system was developed to study the effect of γ-hydroxybutyrate on nerve cell depolarization-induced release of labelled dopamine, noradrenaline and 5-hydroxytryptamine from brain slices.

2. The release of 3H-dopamine formed in rat striatal slices incubated with 3H-tyrosine was followed either by transferring the slices through successive media or by using a superfusion system. A one to three minute exposure to K+ (53 mM) caused up to a thirty fold increase in the release of newly synthesized 3H-dopamine. This K+-induced release was antagonized when γ-hydroxybutyrate (1 × 10-3M) was present in the medium.

3. Potassium (53 mM) increased (eighteen to thirty fold) the release of 3H-dopamine from striatal slices initially loaded by preincubation with 3H-dopamine. However, the K+-induced release of this pool of dopamine was not antagonized by γ-hydroxybutyrate.

4. Potassium (53 mM) also increased the release from striatal slices of 3H-5-hydroxytryptamine newly synthesized from 3H-tryptophan. This K+-induced release of 5-hydroxytryptamine was also not inhibited by γ-hydroxybutyrate.

5. The release of newly synthesized 3H-noradrenaline from hypothalamic slices was also increased by K+. This K+-induced release, however, unlike that of 5-hydroxytryptamine, was antagonized when γ-hydroxybutyrate was present in the superfusion medium.

6. Removal of Ca++ had no effect on K+-induced release of 3H-dopamine when followed by transferring the slices through successive media. This K+-induced release was abolished, however, when Mg++ (12 mM) was present in the medium.

7. The removal of Ca++ from the superfusion medium abolished almost completely the K+-induced release from striatal slices of either newly synthesized 3H-dopamine or preloaded 3H-dopamine. This is presumably due to a more effective washout of tissue Ca++ by the superfusion technique.

8. The ability of γ-hydroxybutyrate to antagonize the K+-induced release of monoamines from brain slices does not appear to be unique to the release of newly synthesized dopamine from the striatum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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