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. 1975 Jun;54(2):205–212. doi: 10.1111/j.1476-5381.1975.tb06930.x

The responses of the isolated, blood-perfused spleen of the dog to angiotensin, oxytocin and vasopressin

BN Davies, PG Withrington
PMCID: PMC1666588  PMID: 1148510

Abstract

1 The responses of the smooth muscle of the capsule and blood vessels of the isolated, blood-perfused spleen of the dog to angiotensin, oxytocin and vasopressin have been investigated and compared to the actions of the catecholamines, adrenaline and noradrenaline.

2 Increasing doses of each of the three polypeptides cause graded increases in splenic vascular resistance and reductions in spleen volume.

3 Doses of the polypeptides which evoked increases in splenic vascular resistance not significantly different from increases produced by chosen doses of each catecholamine caused significantly smaller reductions in spleen volume.

4 The time-course of action of the polypeptides on the splenic vascular smooth muscle is different since the time to 50% recovery from vasopressin is highly significantly longer than that for equieffective doses of either angiotensin or oxytocin.

5 Phenoxybenzamine, in a dose which almost blocked the actions of the catecholamines, increased the responses of the vascular and capsular smooth muscle to oxytocin, vasopressin and angiotensin. This increase was not observed with another α-adrenoceptor blocking agent, phentolamine.

6 The significant species variation in the responses of the smooth muscle of the spleen to polypeptides and catecholamines are discussed and the results are considered in the context of the possible physiological roles of the polypeptides in haemorrhage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aars H., Akre S. Effect of angiotensin on sympathetic nerve activity. Acta Physiol Scand. 1968 Sep-Oct;74(1):134–141. doi: 10.1111/j.1748-1716.1968.tb04222.x. [DOI] [PubMed] [Google Scholar]
  2. Ayers A. B., Davies B. N., Withrington P. G. Responses of the isolated, perfused human spleen to sympathetic nerve stimulation, catecholamines and polypeptides. Br J Pharmacol. 1972 Jan;44(1):17–30. doi: 10.1111/j.1476-5381.1972.tb07234.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ayers A. B., Davies B. N., Withrington P. G. The responses of the isolated perfused spleen of man to sympathetic nerve stimulation, catecholamines and polypeptides. Br J Pharmacol. 1970 Nov;40(3):575P–576P. [PMC free article] [PubMed] [Google Scholar]
  4. Biro G. P., Forsling M. L., Wilmott R. W. Mechanisms of the augmented secretion of vasopressin during haemorrhage in the vagotomized dog. J Physiol. 1972 Apr;222(2):164P–165P. [PubMed] [Google Scholar]
  5. Cohen M. M., Sitar D. S., McNeill J. R., Greenway C. V. Vasopressin and angiotensin on resistance vessels of spleen, intestine, and liver. Am J Physiol. 1970 Jun;218(6):1704–1706. doi: 10.1152/ajplegacy.1970.218.6.1704. [DOI] [PubMed] [Google Scholar]
  6. Davies B. N., Gamble J., Withrington P. G. Frequency-dependent differences in the responses of the capsular and vascular smooth muscle of the spleen of the dog to sympathetic nerve stimulation. J Physiol. 1973 Jan;228(1):13–25. doi: 10.1113/jphysiol.1973.sp010069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davies B. N., Robinson B. H., Withrington P. G. The effects of graded doses of phenoxybenzamine on the vascular and capsular responses of the isolated, blood-perfused dogs spleen to sympathetic nerve stimulation and catecholamines. Arch Int Pharmacodyn Ther. 1969 Jul;180(1):143–154. [PubMed] [Google Scholar]
  8. Davies B. N., Withrington P. G. The effects of prostaglandins E1 and E2 on the smooth muscle of the dog spleen and on its responses to catecholamines, angiotensin and nerve stimulation. Br J Pharmacol Chemother. 1968 Jan;32(1):136–144. doi: 10.1111/j.1476-5381.1968.tb00437.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Distler A., Liebau H., Wolff H. P. Action of angiotensin on sympathetic nerve endings in isolated blood vessels. Nature. 1965 Aug 14;207(998):764–765. doi: 10.1038/207764a0. [DOI] [PubMed] [Google Scholar]
  10. Enero M. A., Langer S. Z., Rothlin R. P., Stefano F. J. Role of the -adrenoceptor in regulating noradrenaline overflow by nerve stimulation. Br J Pharmacol. 1972 Apr;44(4):672–688. doi: 10.1111/j.1476-5381.1972.tb07306.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ferreira S. H., Vane J. R. Half-lives of peptides and amines in the circulation. Nature. 1967 Sep 16;215(5107):1237–1240. doi: 10.1038/2151237a0. [DOI] [PubMed] [Google Scholar]
  12. Greenway C. V., Stark R. D. The vascular responses of the spleen to intravenous infusions of catecholamines, angiotensin and vasopressi in the anaesthetized cat. Br J Pharmacol. 1970 May;38(3):583–592. doi: 10.1111/j.1476-5381.1970.tb10599.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Greenway C. V., Stark R. D. Vascular responses of the spleen to rapid haemorrhage in the anaesthetized cat. J Physiol. 1969 Sep;204(1):169–179. doi: 10.1113/jphysiol.1969.sp008906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hertting G., Suko J. Influence of angiotensin, vasopressin or changes in flow rate on vasoconstriction, changes in volume and [3H]-noradrenaline release following postganglionic sympathetic nerve stimulation in the isolated cat spleen. Br J Pharmacol Chemother. 1966 Mar;26(3):686–696. doi: 10.1111/j.1476-5381.1966.tb01848.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hodge R. L., Lowe R. D., Vane J. R. The effects of alteration of blood-volume on the concentration of circulating angiotensin in anaesthetized dogs. J Physiol. 1966 Aug;185(3):613–626. doi: 10.1113/jphysiol.1966.sp008005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Regoli D., Vane J. R. The continuous estimation of angiotensin formed in the circulation of the dog. J Physiol. 1966 Apr;183(3):513–531. doi: 10.1113/jphysiol.1966.sp007881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schrier R. W., Verroust P. J., Jones J. J., Fabian M., Lee J., De Wardener H. E. Effect of isotonic saline infusion and acute haemorrhage on plasma oxytocin and vasopressin concentrations in dogs. Clin Sci. 1968 Dec;35(3):433–443. [PubMed] [Google Scholar]
  18. Stark R. D., McNeill J. R., Greenway C. V. Sympathetic and hypophyseal roles in the splenic response to hemorrhage. Am J Physiol. 1971 Mar;220(3):837–840. doi: 10.1152/ajplegacy.1971.220.3.837. [DOI] [PubMed] [Google Scholar]
  19. Swearingen K. C., Nicoll C. S. Prolactin turnover in rat adenohypophyses in vivo: its evaluation as a method for estimating secretion rates. J Endocrinol. 1972 Apr;53(1):1–15. doi: 10.1677/joe.0.0530001. [DOI] [PubMed] [Google Scholar]

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