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. 1975 Sep;55(1):57–64. doi: 10.1111/j.1476-5381.1975.tb07610.x

Mediators of passive lung anaphylaxis in the rat.

J B Farmer, I M Richards, P Sheard, A M Woods
PMCID: PMC1666711  PMID: 810194

Abstract

Passive lung anaphylaxis (PLA) was investigated in rats sensitized by the intravenous injection of high titre reaginic antiserum prepared in rats. 2 The effect of various pharmacological antagonists on anaphylactic bronchoconstriction in vivo were examined. An antihistamine (mepyramine), a kallikrein inactivator (aprotinin) or a prostaglandin synthesis inhibitor (aspirin) did not inhibit PLA, whereas an anti-5-hydroxytryptamine agent (methysergide) and an anti-slow reacting substance-A agent (FPL 55712) significantly reduced the response. 3 Isolated perfused lungs taken from sensitized rats released, on challenge with the sensitizing antigen, histamine, 5-hydroxytryptamine, slow reacting substance of anaphylaxis (SRS-A) and prostaglandins, but no rabbit aorta contracting substance (RCS). 4 Disodium cromoglycate inhibited both anaphylactic bronchoconstriction in vivo and the anaphylactic release of mediators in vitro. Inhibition in vivo was dose-related. 5 Mediators from the intestine, the primary shock organ of anaphylaxis in the rat, did not contribute to the lung response. 6 Vagal reflex pathways were found not to be important in PLA in vivo. 7 The relationship between the mediators released following antigen challenge of passively sensitized rat lung in vitro and passive lung anaphylaxis in vivo is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Augstein J., Farmer J. B., Lee T. B., Sheard P., Tattersall M. L. Selective inhibitor of slow reacting substance of anaphylaxis. Nat New Biol. 1973 Oct 17;245(146):215–217. doi: 10.1038/newbio245215a0. [DOI] [PubMed] [Google Scholar]
  2. Aviado D. M., Sadavongvivad C. Pharmacological significance of biogenic amines in the lungs: histamine. Br J Pharmacol. 1970 Feb;38(2):366–373. doi: 10.1111/j.1476-5381.1970.tb08524.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BROCKLEHURST W. E. The release of histamine and formation of a slow-reacting substance (SRS-A) during anaphylactic shock. J Physiol. 1960 Jun;151:416–435. doi: 10.1113/jphysiol.1960.sp006449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burden D. T., Parkes M. W., Gardiner D. G. Effect of beta-adrenoceptive blocking agents on the response to bronchoconstrictor drugs in the guinea-pig air overflow preparation. Appendix describing a new modification of the air overflow method. Br J Pharmacol. 1971 Jan;41(1):122–131. doi: 10.1111/j.1476-5381.1971.tb09942.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Church M. K., Collier H. O., James G. W. The inhibition by dexamethasone and disodium cromoglycate of anaphylactic bronchoconstriction in the rat. Br J Pharmacol. 1972 Sep;46(1):56–65. doi: 10.1111/j.1476-5381.1972.tb06848.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cox J. S. Disodium cromoglycate. Mode of action and its possible relevance to the clinical use of the drug. Br J Dis Chest. 1971 Oct;65(4):189–204. doi: 10.1016/0007-0971(71)90028-3. [DOI] [PubMed] [Google Scholar]
  7. Csaba B., Went M. Role of histamine, 5-hydroxytryptamine and bradykinin in the rat anaphylaxis. Acta Physiol Acad Sci Hung. 1971;39(4):369–374. [PubMed] [Google Scholar]
  8. Dawson W., Starr M. S., West G. B. Inhibition of anaphylactic shock in the rat by antihistamines and ascorbic acid. Br J Pharmacol Chemother. 1966 Aug;27(2):249–255. doi: 10.1111/j.1476-5381.1966.tb01660.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gold W. M., Kessler G. F., Yu D. Y. Role of vagus nerves in experimental asthma in allergic dogs. J Appl Physiol. 1972 Dec;33(6):719–725. doi: 10.1152/jappl.1972.33.6.719. [DOI] [PubMed] [Google Scholar]
  10. Goose J., Blair A. M. Passive cutaneous anaphylaxis in the rat, induced with two homologous reagin-like antibodies and its specific inhibition with disodium cromoglycate. Immunology. 1969 Jun;16(6):749–760. [PMC free article] [PubMed] [Google Scholar]
  11. Horton E. W. Hypotheses on physiological roles of prostaglandins. Physiol Rev. 1969 Jan;49(1):122–161. doi: 10.1152/physrev.1969.49.1.122. [DOI] [PubMed] [Google Scholar]
  12. Itkin I. H., Anand S. C. The role of atropine as a mediator blocker of induced bronchial obstruction. J Allergy. 1970 Mar;45(3):178–186. doi: 10.1016/0021-8707(70)90126-7. [DOI] [PubMed] [Google Scholar]
  13. Karczewski W., Widdicombe J. G. The role of the vagus nerves in the respiratory and circulatory reactions to anaphylaxis in rabbits. J Physiol. 1969 Apr;201(2):293–304. doi: 10.1113/jphysiol.1969.sp008756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kooman W. J., Orange R. P., Austen K. F. Immunochemical and biologic properties of rat IgE. 3. Modulation of the IgE-mediated release of slow-reacting substance of anaphylaxis by agents influencing the level of cyclic 3',5'-adenosine monoposphate. J Immunol. 1970 Nov;105(5):1096–1102. [PubMed] [Google Scholar]
  15. MAIN I. H. THE INHIBITORY ACTIONS OF PROSTAGLANDINS ON RESPIRATORY SMOOTH MUSCLE. Br J Pharmacol Chemother. 1964 Jun;22:511–519. doi: 10.1111/j.1476-5381.1964.tb01705.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mills J. E., Widdicombe J. G. Role of the vagus nerves in anaphylaxis and histamine-induced bronchoconstrictions in guinea-pigs. Br J Pharmacol. 1970 Aug;39(4):724–731. doi: 10.1111/j.1476-5381.1970.tb09898.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Orange R. P., Stechschulte D. J., Austen K. F. Immunochemical and biologic properties of rat IgE. II. Capacity to mediate the immunologic releas of histamine an slow-reacting substance of anaphylaxis (SRS-A). J Immunol. 1970 Nov;105(5):1087–1095. [PubMed] [Google Scholar]
  18. Orr T. S., Blair A. M. Potentiated reagin response to egg albumin and conalbumin in Nippostrongylus brasiliensis infected rats. Life Sci. 1969 Oct 15;8(20):1073–1077. doi: 10.1016/0024-3205(69)90459-7. [DOI] [PubMed] [Google Scholar]
  19. PARRATT J. R., WEST G. B. 5-Hydroxytryptamine and tissue mast cells. J Physiol. 1957 Jul 11;137(2):169–178. doi: 10.1113/jphysiol.1957.sp005804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Piper P. J., Vane J. R. Release of additional factors in anaphylaxis and its antagonism by anti-inflammatory drugs. Nature. 1969 Jul 5;223(5201):29–35. doi: 10.1038/223029a0. [DOI] [PubMed] [Google Scholar]
  21. Piper P. J., Walker J. L. The release of spasmogenic substances from human chopped lung tissue and its inhibition. Br J Pharmacol. 1973 Feb;47(2):291–304. doi: 10.1111/j.1476-5381.1973.tb08327.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Roy A. C., Warren B. Y. Inhibition of cAMP phosphodiesterase by disodium cromoglycate. Biochem Pharmacol. 1974 Feb 15;23(4):917–920. doi: 10.1016/0006-2952(74)90223-8. [DOI] [PubMed] [Google Scholar]
  23. SANYAL R. K., WEST G. B. Anaphylactic shock in the albino rat. J Physiol. 1958 Aug 6;142(3):571–584. doi: 10.1113/jphysiol.1958.sp006037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sadavongvivad C. Pharmacological significance of biogenic amines in the lungs: 5-hydroxytryptamine. Br J Pharmacol. 1970 Feb;38(2):353–365. doi: 10.1111/j.1476-5381.1970.tb08523.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Taylor W. A., Francis D. H., Sheldon D., Roitt I. M. The anti-anaphylactic actions of disodium cromoglycate, theophylline, isoprenaline and prostaglandins. Int Arch Allergy Appl Immunol. 1974;46(1):104–120. doi: 10.1159/000231116. [DOI] [PubMed] [Google Scholar]
  26. WEISSBACH H., WAALKES T. P., UDENFRIEND S. A simplified method for measuring serotonin in tissues; simultaneous assay of both serotonin and histamine. J Biol Chem. 1958 Feb;230(2):865–871. [PubMed] [Google Scholar]
  27. WEST G. B. Some factors involved in anaphylactic shock. Int Arch Allergy Appl Immunol. 1959;15:231–236. doi: 10.1159/000229048. [DOI] [PubMed] [Google Scholar]
  28. Yu D. Y., Galant S. P., Gold W. M. Inhibition of antigen-induced bronchoconstriction by atropine in asthmatic patients. J Appl Physiol. 1972 Jun;32(6):823–828. doi: 10.1152/jappl.1972.32.6.823. [DOI] [PubMed] [Google Scholar]

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