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. 1978 Oct;64(2):315–320. doi: 10.1111/j.1476-5381.1978.tb17307.x

Recirculation of prostacyclin (PGI2) in the dog.

G J Dusting, S Moncada, J R Vane
PMCID: PMC1668311  PMID: 361136

Abstract

1 The inactivation of prostacyclin (PGI2) in the circulation of anaesthetized dogs has been studied by the blood-bathed organ bioassay technique. 2 Spiral strips of bovine coronary and rabbit coeliac or mesenteric artery detected concentrations of PGI2 of 2 to 5 ng/ml. These tissues were insensitive to concentrations at least 200 fold higher of 15-oxo-PGI2 and 6-oxo-PGF1alpha. 3 PGI2 assayed on bovine coronary artery, rabbit coeliac artery or rat stomach strip, had a half life in blood of 3.0 +/- 0.3 min, indicating non-enzymatic degradation. 4 No disappearance could be detected by bovine coronary artery when PGI2 was infused across the lungs (0.1 to 0.5 microgram kg-1 min-1). However, PGI2 was partially inactivated in passage through vascular beds of hindquarters and liver. 5 Of PGI2 infused into the aorta 35 to 65% escaped inactivation in one complete circulation. Therefore, endogenous PGI2 released from the lungs may function as a circulating hormone.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong J. M., Chapple D., Dusting G. J., Hughes R., Moncada S., Vane J. R. Cardiovascular actions of prostacyclin (PCI2) in chloralose anaesthetized dogs [proceedings]. Br J Pharmacol. 1977 Sep;61(1):136P–136P. [PMC free article] [PubMed] [Google Scholar]
  2. Armstrong J. M., Lattimer N., Moncada S., Vane J. R. Comparison of the vasodepressor effects of prostacyclin and 6-oxo-prostaglandin F1alpha with those of prostaglandin E2 in rats and rabbits. Br J Pharmacol. 1978 Jan;62(1):125–130. doi: 10.1111/j.1476-5381.1978.tb07014.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cho M. J., Allen M. A. Chemical stability of prostacyclin (PGI2) in aqueous solutions. Prostaglandins. 1978 Jun;15(6):943–954. doi: 10.1016/0090-6980(78)90037-0. [DOI] [PubMed] [Google Scholar]
  4. Dusting G. J., Moncada S., Vane J. R. Disappearance of prostacyclin (PGI2) in the circulation of the dog [proceedings]. Br J Pharmacol. 1978 Mar;62(3):414P–415P. [PMC free article] [PubMed] [Google Scholar]
  5. Dusting G. J., Moncada S., Vane J. R. Prostacyclin (PGX) is the endogenous metabolite responsible for relaxation of coronary arteries induced by arachindonic acid. Prostaglandins. 1977 Jan;13(1):3–15. doi: 10.1016/0090-6980(77)90037-5. [DOI] [PubMed] [Google Scholar]
  6. Dusting G. J., Moncada S., Vane J. R. Vascular actions of arachidonic acid and its metabolites in perfused mesenteric and femoral beds of the dog. Eur J Pharmacol. 1978 May 1;49(1):65–72. doi: 10.1016/0014-2999(78)90222-4. [DOI] [PubMed] [Google Scholar]
  7. Ferreira S. H., Vane J. R. Prostaglandins: their disappearance from and release into the circulation. Nature. 1967 Dec 2;216(5118):868–873. doi: 10.1038/216868a0. [DOI] [PubMed] [Google Scholar]
  8. Gryglewski R. J., Korbut R., Ocetkiewicz A. Generation of prostacyclin by lungs in vivo and its release into the arterial circulation. Nature. 1978 Jun 29;273(5665):765–767. doi: 10.1038/273765a0. [DOI] [PubMed] [Google Scholar]
  9. Johnson R. A., Lincoln F. H., Thompson J. L., Nidy E. G., Mizak S. A., Axen U. Synthesis and sterochemistry of prostacyclin and synthesis of 6-ketoprostagliandin F1alpha. J Am Chem Soc. 1977 Jun 8;99(12):4182–4184. doi: 10.1021/ja00454a060. [DOI] [PubMed] [Google Scholar]
  10. Kulkarni P. S., Roberts R., Needleman P. Paradoxical endogenous synthesis of a coronary dilating substance from arachidonate. Prostaglandins. 1976 Sep;12(3):337–353. doi: 10.1016/0090-6980(76)90015-0. [DOI] [PubMed] [Google Scholar]
  11. Moncada S., Gryglewski R., Bunting S., Vane J. R. An enzyme isolated from arteries transforms prostaglandin endoperoxides to an unstable substance that inhibits platelet aggregation. Nature. 1976 Oct 21;263(5579):663–665. doi: 10.1038/263663a0. [DOI] [PubMed] [Google Scholar]
  12. Moncada S., Korbut R., Bunting S., Vane J. R. Prostacyclin is a circulating hormone. Nature. 1978 Jun 29;273(5665):767–768. doi: 10.1038/273767a0. [DOI] [PubMed] [Google Scholar]
  13. Moncada S., Mugridge K. G., Whittle B. J. The differential response of a novel bioassay tissue, the rabbit transverse stomach-strip, to prostacyclin (PGI2) and other prostaglandins [proceedings]. Br J Pharmacol. 1977 Nov;61(3):451P–452P. [PMC free article] [PubMed] [Google Scholar]
  14. VANE J. R. THE USE OF ISOLATED ORGANS FOR DETECTING ACTIVE SUBSTANCES IN THE CIRCULATING BLOOD. Br J Pharmacol Chemother. 1964 Oct;23:360–373. doi: 10.1111/j.1476-5381.1964.tb01592.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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