Abstract
A new method is described for preparing direct mitotic chromosome spreads from chorionic villous samples, which has resulted in sufficiently high yields of well-banded metaphases to permit a complete standard chromosomal diagnosis in 20 of 20 cases. A method of establishing monolayer cultures from this material that can be harvested from 3 to 7 days after initiation is also presented.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blakemore K. J., Samuelson J., Breg W. R., Mahoney M. J. Maternal metaphases on direct chromosome preparation of first trimester decidua. Hum Genet. 1985;69(4):380–380. doi: 10.1007/BF00291660. [DOI] [PubMed] [Google Scholar]
- Simoni G., Brambati B., Danesino C., Rossella F., Terzoli G. L., Ferrari M., Fraccaro M. Efficient direct chromosome analyses and enzyme determinations from chorionic villi samples in the first trimester of pregnancy. Hum Genet. 1983;63(4):349–357. doi: 10.1007/BF00274761. [DOI] [PubMed] [Google Scholar]