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. 1981 Nov;33(6):916–927.

Feline mucopolysaccharidosis VI: purification and characterization of the resident arylsulfatase B activity.

D T Vine, M M McGovern, M E Haskins, R J Desnick
PMCID: PMC1685156  PMID: 6798861

Abstract

Hepatic arylsulfatase B (ASB) from normal and mucopolysaccharidosis VI (MPS VI) cats was purified over 2,800- and 1,800-fold, respectively, and their physical and kinetic properties were characterized. In contrast to the normal feline enzyme, the partially purified MPS VI residual activity had a 100-fold greater Km value and was markedly less stable to thermal, cryo-, and pH-inactivation. In addition, the MPS VI enzyme had a more negative charge as determined by its migration on polyacrylamide gel electrophoresis and its elution profile on cation exchange chromatography. Finally, the MPS VI activity had approximately half the apparent molecular weight of the normal feline enzyme, which was a homodimer, suggesting that the genetic mutation in feline MPS VI altered the subunit association as well as the kinetic and stability properties of the mutant protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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