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. 1976 Jan;31(1):35–38. doi: 10.1128/aem.31.1.35-38.1976

Microtiter assay for interferon: microspectrophotometric quantitation of cytopathic effect.

N H McManus
PMCID: PMC169714  PMID: 182074

Abstract

This report describes an accurate, reproducible, and efficient microassay for human interferon, using a dye-uptake method to quantitate cytopathogenicity. The antiviral activity was measured by using a Gilford 300-N microsepctrophotometer, with the automatic programmer, sampler, and data lister. Two hundred interferon samples, each in a final volume of 1.0 ml, may be analyzed and recorded in 45 min. The reproducibility of a laboratory reference interferon and the human international reference B 69/19 on two different cell lines, using the model Q Oxford sampler, was found to be excellent, with the standard error of the log10 geometric mean of both references +/- 0.04 to 0.06.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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