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. 1976 Feb;31(2):182–188. doi: 10.1128/aem.31.2.182-188.1976

Method for reproducible large-volume production and purification of Rauscher murine leukemia virus.

R W Johnson, A Perry, O R Robinson Jr, G P Shibley
PMCID: PMC169745  PMID: 63267

Abstract

Rauscher murine leukemia virus was produced in roller-bottle cultures of chronically infected JLS-V9 cells. Virus from this culture fluid was concentrated and purified by two semi-isopycnic bandings in sucrose gradients. Virus material obtained from young, nonconfluent cultures (early-harvest virus) yielded products characteristically containing endogenous ribonucleic acid-dependent deoxyribonucleic acid polymerase with high specific activity (400 to 1,000 pmol of [3H]thymidine 5'-triphosphate incorporated per milligram of protein per hour). Fluids obtained from older confluent cultures (late-harvest virus) yielded products with endogenous ribonucleic acid-dependent deoxyribonucleic acid polymerase with little or no specific activity (200 pmol or less of [3H]thymidine 5'-triphosphate incorporated per milligram of protein per hour), but with higher virus particle counts and greater amounts of protein and gs antigen than the early-harvest products.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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