Abstract
TNT (2,4,6-trinitrotoluene) of explosive grade is highly toxic to marine forms that included fresh water unicellular green algae (Selenastrum capricornutum), tidepool copepods (Tigriopus californicus), and oyster larvae (Crassostrea gigas), and mutagenic to Salmonella typhimurium. On the basis of mutagenic assays carried out with a set of histidine-requiring strains of the bacterium, TNT was detected as a frameshift mutagen that significantly accelerates the reversion rate of a frameshift tester, TA-98. In contrast, the major microbial metabolites of TNT appeared to be nontoxic and nonmutagenic.
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- Ames B. N., Durston W. E., Yamasaki E., Lee F. D. Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2281–2285. doi: 10.1073/pnas.70.8.2281. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ames B. N., Lee F. D., Durston W. E. An improved bacterial test system for the detection and classification of mutagens and carcinogens. Proc Natl Acad Sci U S A. 1973 Mar;70(3):782–786. doi: 10.1073/pnas.70.3.782. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Channon H. J., Mills G. T., Williams R. T. The metabolism of 2:4:6-trinitrotoluene (alpha-T.N.T.). Biochem J. 1944;38(1):70–85. doi: 10.1042/bj0380070. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MacPhee D. G. Salmonella typhimurium hisG46 (R-Utrecht): possible use in screening mutagens and carcinogens. Appl Microbiol. 1973 Dec;26(6):1004–1005. doi: 10.1128/am.26.6.1004-1005.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCann J., Spingarn N. E., Kobori J., Ames B. N. Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids. Proc Natl Acad Sci U S A. 1975 Mar;72(3):979–983. doi: 10.1073/pnas.72.3.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
- Won W. D., Heckly R. J., Glover D. J., Hoffsommer J. C. Metabolic disposition of 2,4,6-trinitrotoluene. Appl Microbiol. 1974 Mar;27(3):513–516. doi: 10.1128/am.27.3.513-516.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]