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. 1976 Apr;31(4):605–608. doi: 10.1128/aem.31.4.605-608.1976

Continuously variable amplitude contrast microscopy for the detection and study of microorganisms in soil.

L E Casida Jr
PMCID: PMC169825  PMID: 773307

Abstract

A new type of phase microscope was used to detect and observe the microflora in soil and to differentiate cells and spores from soil debris. This microscope provides a continuous variation in the amplitude ratios between undeviated and deviated beams of light, and the microbial cells, spores, and debris in soil change in appearance to a differing degree in response to the changes in amplitude ratio.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALEXANDER F. E., JACKSON R. M. Examination of soil micro-organisms in their natural environment. Nature. 1954 Oct 16;174(4433):750–751. doi: 10.1038/174750b0. [DOI] [PubMed] [Google Scholar]
  2. Babiuk L. A., Paul E. A. The use of fluorescein isothiocyanate in the determination of the bacterial biomass of grassland soil. Can J Microbiol. 1970 Feb;16(2):57–62. doi: 10.1139/m70-011. [DOI] [PubMed] [Google Scholar]
  3. CASIDA L. E., Jr On the isolation and growth of individual microbial cells from soil. Can J Microbiol. 1962 Feb;8:115–119. doi: 10.1139/m62-015. [DOI] [PubMed] [Google Scholar]
  4. Casida L. E., Jr Infrared color photography: selective demonstration of bacteria. Science. 1968 Jan 12;159(3811):199–200. doi: 10.1126/science.159.3811.199. [DOI] [PubMed] [Google Scholar]
  5. Casida L. E., Jr Infrared color photomicrography of soil microorganisms. Can J Microbiol. 1975 Nov;21(11):1892–1893. doi: 10.1139/m75-276. [DOI] [PubMed] [Google Scholar]
  6. Casida L. E., Jr Interval scanning photomicrography of microbial cell populations. Appl Microbiol. 1972 Jan;23(1):190–192. doi: 10.1128/am.23.1.190-192.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Casida L. E., Jr Microorganisms in unamended soil as observed by various forms of microscopy and staining. Appl Microbiol. 1971 Jun;21(6):1040–1045. doi: 10.1128/am.21.6.1040-1045.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Casida L. E., Jr Observation of microorganisms in soil and other natural habitats. Appl Microbiol. 1969 Dec;18(6):1065–1071. doi: 10.1128/am.18.6.1065-1071.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Conn H. J. USE OF THE MICROSCOPE IN STUDYING THE ACTIVITIES OF BACTERIA IN SOIL. J Bacteriol. 1929 Jun;17(6):399–405. doi: 10.1128/jb.17.6.399-405.1929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McElroy L. J., Casida L. E., Jr An evaluation of rhodamine-labeled lysozyme as a fluorescent stain for in situ soil bacteria. Can J Microbiol. 1972 Jun;18(6):933–936. doi: 10.1139/m72-143. [DOI] [PubMed] [Google Scholar]
  11. Millar W. N., Casida L. E., Jr Microorganisms in soil as observed by staining with rhodamine-labeled lysozyme. Can J Microbiol. 1970 May;16(5):305–307. doi: 10.1139/m70-055. [DOI] [PubMed] [Google Scholar]
  12. Pital A., Janowitz S. L., Hudak C. E., Lewis E. E. Direct fluorescent labeling of microorganisms as a possible life-detection technique. Appl Microbiol. 1966 Jan;14(1):119–123. doi: 10.1128/am.14.1.119-123.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SKINNER F. A., JONES P. C., MOLLISON J. E. A comparison of a direct- and a plate counting technique for the quantitative estimation of soil micro-organisms. J Gen Microbiol. 1952 May;6(3-4):261–271. doi: 10.1099/00221287-6-3-4-261. [DOI] [PubMed] [Google Scholar]

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