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. 1976 Jul;32(1):85–88. doi: 10.1128/aem.32.1.85-88.1976

Preparation of phase 1Q fever antigen suitable for vaccine use.

D S Spicer, A N DeSanctis
PMCID: PMC170010  PMID: 970938

Abstract

Five lots of phase 1 Q fever antigen suitable for use as vaccine were prepared utilizing the Freon-brushite method of antigen purification. Antigen concentration levels were initially adjusted turbidimetrically and later confirmed by antibody response in guinea pigs. Data showed that all lots were essentially identical in purity and antigenicity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berman S., Gochenour R. B., Cole G., Lowenthal J. P., Benenson A. S. METHOD FOR THE PRODUCTION OF A PURIFIED DRY Q FEVER VACCINE. J Bacteriol. 1961 May;81(5):794–799. doi: 10.1128/jb.81.5.794-799.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Canonico P. G., Van Zwieten M. J., Christmas W. A. Purification of large quantities of coxiella burnetii rickettsia by density gradient zonal centrifugation. Appl Microbiol. 1972 May;23(5):1015–1022. doi: 10.1128/am.23.5.1015-1022.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dubois D. R., Cutchins E. C., Berman S., Lowenthal J. P., Timchak R. L. Preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow ultracentrifugation. Appl Microbiol. 1972 May;23(5):841–845. doi: 10.1128/am.23.5.841-845.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fiset P., Ormsbee R. A., Silberman R., Peacock M., Spielman S. H. A microagglutination technique for detection and measurement of rickettsial antibodies. Acta Virol. 1969 Jan;13(1):60–66. [PubMed] [Google Scholar]
  5. GIMENEZ D. F. STAINING RICKETTSIAE IN YOLK-SAC CULTURES. Stain Technol. 1964 May;39:135–140. doi: 10.3109/10520296409061219. [DOI] [PubMed] [Google Scholar]
  6. JACKSON E. B., SMADEL J. E. Immunization against scrub typhus. II. Preparation of lyophilized living vaccine. Am J Hyg. 1951 May;53(3):326–331. doi: 10.1093/oxfordjournals.aje.a119457. [DOI] [PubMed] [Google Scholar]
  7. LACKMAN D. B., BELL E. J., BELL J. F., PICKENS E. G. Intradermal sensitivity testing in man with a purified vaccine for Q fever. Am J Public Health Nations Health. 1962 Jan;52:87–93. doi: 10.2105/ajph.52.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. ORMSBEE R. A., BELL E. J., LACKMAN D. B., TALLENT G. THE INFLUENCE OF PHASE ON THE PROTECTIVE POTENCY OF Q FEVER VACCINE. J Immunol. 1964 Mar;92:404–412. [PubMed] [Google Scholar]
  9. STOKER M. G., FISET P. Phase variation of the Nine Mile and other strains of Rickettsia burneti. Can J Microbiol. 1956 May;2(3):310–321. doi: 10.1139/m56-036. [DOI] [PubMed] [Google Scholar]
  10. Spicer D. S., DeSanctis A. N., Beiler J. M. Preparation of highly purified concentrates of Coxiella burneti. Proc Soc Exp Biol Med. 1970 Dec;135(3):706–708. doi: 10.3181/00379727-135-35125. [DOI] [PubMed] [Google Scholar]

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