Abstract
1. A simple and reliable method is described for assaying 1 pg or less of acetylcholine (ACh) using a strip of clam (Mya arenaria) heart.
2. Clam heart preparations generally are extremely selective for ACh. The preparation described is at least 105 times less sensitive to the following: acetyl coenzyme A, adrenaline, choline, dopamine, γ-aminobutyric acid, glutamic acid, histamine, 5-hydroxytryptamine and noradrenaline.
3. Because this preparation is one of the most sensitive known for detecting ACh, it should prove useful for assaying particularly low levels of this substance in biological extracts.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cottrell G. A., Pentreath V. W., Powell B. Is the Mya arenaria heart suitable for ACh bioassay? Comp Biochem Physiol. 1968 Dec;27(3):787–794. doi: 10.1016/0010-406x(68)90618-x. [DOI] [PubMed] [Google Scholar]
- WELSH J. H., TWAROG B. Measurement of smooth muscle activity in invertebrate animals. Methods Med Res. 1960;8:187–199. [PubMed] [Google Scholar]
- Whittaker V. P., Michaelson I. A., Kirkland R. J. The separation of synaptic vesicles from nerve-ending particles ('synaptosomes'). Biochem J. 1964 Feb;90(2):293–303. doi: 10.1042/bj0900293. [DOI] [PMC free article] [PubMed] [Google Scholar]