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. 1976 Oct;32(4):585–591. doi: 10.1128/aem.32.4.585-591.1976

Quantitative requirements for exponential growth of Alcaligenes eutrophus.

R Repaske, A C Repaske
PMCID: PMC170311  PMID: 984831

Abstract

Quantitative nutrient requirements for unrestricted autotrophic growth of Alcaligenes eutrophus were determined. Minimum saturating concentrations of Mg2+, SO42-, PO43-, Fe3+, and Na2+ for an optical density increase of 2 were 10(-4) M 8 X10(-5) M, 5 X 10(-4) to 6 X 10(-4) M, 10(-5) M, and 10(-7) to 2 X 10(-7) M, respectively. Trace metal requirements for cobalt, chromium, and copper were also demonstrated, but minimum concentrations could not be determined because other reagents contributed a high background of these metals. Under certain conditions an apparent response to zinc was observed, although other experiments suggest the zinc salt contained another metal that was required for growth. Poly-beta-hydroxybutyrate biosynthesis was shown to be initiated by a magnesium or sulfate deficiency as well as by a nitrogen or phosphate deficiency.

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Selected References

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  1. BARTHA R., ORDAL E. J. NICKEL-DEPENDENT CHEMOLITHOTROPHIC GROWTH OF TWO HYDROGENOMONAS STRAINS. J Bacteriol. 1965 Apr;89:1015–1019. doi: 10.1128/jb.89.4.1015-1019.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davis D. H., Stanier R. Y., Doudoroff M., Mandel M. Taxonomic studies on some gram negative polarly flagellated "hydrogen bacteria" and related species. Arch Mikrobiol. 1970;70(1):1–13. doi: 10.1007/BF00691056. [DOI] [PubMed] [Google Scholar]
  3. GERHARDT P., GALLUP D. M. DIALYSIS FLASK FOR CONCENTRATED CULTURE OF MICROORGANISMS. J Bacteriol. 1963 Nov;86:919–929. doi: 10.1128/jb.86.5.919-929.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kesler T. G., Voitovich Ia V., Anistratova N. A., Trubachev I. N., Eroshin N. S. Rost i biokhimicheskii sostav vodorodnykh bakterii v usloviiakh blokirovaniia biosinteza biogennymi élementami. Mikrobiologiia. 1972 May-Jun;41(3):456–460. [PubMed] [Google Scholar]
  5. Koch A. L., Putnam S. L. Sensitive biuret method for determination of protein in an impure system such as whole bacteria. Anal Biochem. 1971 Nov;44(1):239–245. doi: 10.1016/0003-2697(71)90366-6. [DOI] [PubMed] [Google Scholar]
  6. LAW J. H., SLEPECKY R. A. Assay of poly-beta-hydroxybutyric acid. J Bacteriol. 1961 Jul;82:33–36. doi: 10.1128/jb.82.1.33-36.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. REPASKE R. Nutritional requirements for Hydrogenomonas eutropha. J Bacteriol. 1962 Feb;83:418–422. doi: 10.1128/jb.83.2.418-422.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Repaske R., Ambrose C. A., Repaske A. C., De Lacy M. L. Bicarbonate requirement for elimination of the lag period of Hydrogenomonas eutropha. J Bacteriol. 1971 Sep;107(3):712–717. doi: 10.1128/jb.107.3.712-717.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Repaske R., Mayer R. Dense autotrophic cultures of Alcaligenes eutrophus. Appl Environ Microbiol. 1976 Oct;32(4):592–597. doi: 10.1128/aem.32.4.592-597.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SCHLEGEL H. G., GOTTSCHALK G., VON BARTHA R. Formation and utilization of poly-beta-hydroxybutyric acid by Knallgas bacteria (Hydrogenomonas). Nature. 1961 Jul 29;191:463–465. doi: 10.1038/191463a0. [DOI] [PubMed] [Google Scholar]
  11. STICKLAND L. H. The determination of small quantities of bacteria by means of the biuret reaction. J Gen Microbiol. 1951 Oct;5(4):698–703. doi: 10.1099/00221287-5-4-698. [DOI] [PubMed] [Google Scholar]
  12. Schultz J. S., Gerhardt P. Dialysis culture of microorganisms: design, theory, and results. Bacteriol Rev. 1969 Mar;33(1):1–47. doi: 10.1128/br.33.1.1-47.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TYRRELL E. A., MACDONALD R. E., GERHARDT P. Biphasic system for growing bacteria in concentrated culture. J Bacteriol. 1958 Jan;75(1):1–4. doi: 10.1128/jb.75.1.1-4.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WALDE E. [Studies on growth and synthesis of stored substance by Hydrogenomonas]. Arch Mikrobiol. 1962;43:109–137. [PubMed] [Google Scholar]
  15. WILLIAMSON D. H., WILKINSON J. F. The isolation and estimation of the poly-beta-hydroxybutyrate inclusions of Bacillus species. J Gen Microbiol. 1958 Aug;19(1):198–209. doi: 10.1099/00221287-19-1-198. [DOI] [PubMed] [Google Scholar]

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