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. 1976 Nov;32(5):653–658. doi: 10.1128/aem.32.5.653-658.1976

Reconcentration of poliovirus from sewage.

S Farrah, C Wallis, P T Shaffer, J L Melnick
PMCID: PMC170378  PMID: 10842

Abstract

Virus can be adsorbed from effluents of sewage treatment plants on large-surface membranes. Subsequent elution of virus requires large volumes, which in turn requires reconcentration of virus for assay. However, reconcentration of such viral eluates on small adsorbent surfaces is difficult because certain soluble sewage components are adsorbed along with the virus on the initial virus adsorbent and are removed along with the virus by the eluent. Upon acidification of the initial eluate to reconcentrate the virus on smaller membrane surfaces, flocs are formed that interfere with the reconcentration process. To circumvent this problem, the interfering sewage components can be removed by activated carbon and ion-exchange resins. The virus is then readily reconcentrated on small membranes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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