Abstract
The isolated vas deferens of the guinea-pig contracted when stimulated transmurally with parallel wire electrodes. These contractions persisted in concentrations of hexamethonium, pentolinium, nicotine and mecamylamine which at the same time abolished the responses to hypogastric nerve stimulation. Procaine and lignocaine in local anaesthetic concentrations abolished the responses to transmural stimulation but potentiated the contractions produced by added noradrenaline. Guanethidine and bretylium in concentrations specific for adrenergic neurone blockade abolished the contractions due to transmural stimulation without impairing the responses of the muscle to added noradrenaline or acetylcholine. In contrast, high concentrations of the adrenergic-blocking agents phentolamine and dihydroergotamine were needed to block the contractions due to transmural stimulation; these concentrations also blocked the response to added noradrenaline but simultaneously reduced the responses to added acetylcholine or potassium chloride. Preparations from guinea-pigs previously treated with reserpine at first responded normally to transmural stimulation; thereafter the contractions diminished progressively but were never abolished. Hyoscine and atropine produced a small decrease in the response to transmural stimulation when present in concentrations up to 1×10-5 and a larger decrease only in concentrations of 1×10-4 or greater. Hemicholinium produced a small decrease of the contractions due to transmural stimulation in concentrations up to 1×10-4; concentrations of 5×10-4 present for 1 hr produced only a slightly greater reduction in response. These experiments show that when the guinea-pig vas deferens is removed without the hypogastric nerve and stimulated transmurally by the method described, contractions are produced mainly by excitation of postganglionic adrenergic nerves.
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Selected References
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- BENTLEY G. A., SABINE J. R. THE EFFECTS OF GANGLION-BLOCKING AND POSTGANGLIONIC SYMPATHOLYTIC DRUGS ON PREPARATIONS OF THE GUINEA-PIG VAS DEFERENS. Br J Pharmacol Chemother. 1963 Aug;21:190–201. doi: 10.1111/j.1476-5381.1963.tb01515.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAY M. D. Effect of sympathomimetic amines on the blocking action of guanethidine, bretylium and xylocholine. Br J Pharmacol Chemother. 1962 Apr;18:421–439. doi: 10.1111/j.1476-5381.1962.tb01421.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OHLIN P., STROMBLAD B. C. Observations on the isolated vas deferens. Br J Pharmacol Chemother. 1963 Apr;20:299–306. doi: 10.1111/j.1476-5381.1963.tb01469.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PATON W. D. The response of the guineapig ileum to electrical stimulation by coaxial electrodes. J Physiol. 1955 Feb 28;127(2):40–1P. [PubMed] [Google Scholar]
- SJOSTRAND N. O. Inhibition by ganglionic blocking agents of the motor response of the isolated guinea-pig vas deferens to hypogastric nerve stimulation. Acta Physiol Scand. 1962 Mar-Apr;54:306–315. doi: 10.1111/j.1748-1716.1962.tb02354.x. [DOI] [PubMed] [Google Scholar]