Abstract
Production of extracellular maltase was studied with thermophilic Bacillus sp. KP 1035, which was selected as the organism producing the highest levels of maltase. The final enzyme yield was increased by maltose, peptone, and yeast extract but reduced by succinate and fumarate. Maximum enzyme production was achieved at 55 degrees C and at an initial pH of 6.2 to 7.0 on a medium containing 0.3% maltose, 1% peptone, 0.1% meat extract, 0.3% yeast extract, 0.3% KH2PO4, and 0.1% KH2PO4. Maltase was synthesized in cytoplasm and accumulated as a large pool during the logarithmic growth phase, which preceded sporulation. At the end of this phase, the enzyme appeared in the culture broth, and its accumulation increased in parallel with a rise in the extracellular protein level. Maltase was stable for 24 h at 60 degrees C over a pH range of 5.6 to 9.0 and retained 95% of the original activity after treatment for 20 min at 70 degrees C at pH 6.8.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Guffanti A. A., Corpe W. A. Maltose metabolism of Pseudomonas fluorescens. J Bacteriol. 1975 Oct;124(1):262–268. doi: 10.1128/jb.124.1.262-268.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guntermann U., Tan I., Hüttermann A. Induction of alpha-glucosidase and synthesis during the cell cycle of Myxobacter AL-1. J Bacteriol. 1975 Oct;124(1):86–91. doi: 10.1128/jb.124.1.86-91.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HALVORSON H. O., WINDERMAN S., GORMAN J. Comparison of the alpha-glucosidases of Saccharomyces produced in response to five non-allelic maltose genes. Biochim Biophys Acta. 1963 Jan 8;67:42–53. doi: 10.1016/0006-3002(63)91795-5. [DOI] [PubMed] [Google Scholar]
- HALVORSON H., ELLIAS L. The purification and properties of an alpha-glucosidase of Saccharomyces italicus Y1225. Biochim Biophys Acta. 1958 Oct;30(1):28–40. doi: 10.1016/0006-3002(58)90237-3. [DOI] [PubMed] [Google Scholar]
- Khan N. A., Eaton N. R. Purification and characterization of maltase and alpha-methyl glucosidase from yeast. Biochim Biophys Acta. 1967 Sep 12;146(1):173–180. doi: 10.1016/0005-2744(67)90084-8. [DOI] [PubMed] [Google Scholar]
- Saito N., Yamamoto K. Regulatory factors affecting alpha-amylase production in bacillus licheniformis. J Bacteriol. 1975 Mar;121(3):848–856. doi: 10.1128/jb.121.3.848-856.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suzuki Y., Kishigami T., Abe S. Production of extracellular alpha-glucosidase by a thermophilic Bacillus species. Appl Environ Microbiol. 1976 Jun;31(6):807–812. doi: 10.1128/aem.31.6.807-812.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang L. H., Hartman P. A. Purification and some properties of an extracellular maltase from Bacillus subtilis. Appl Environ Microbiol. 1976 Jan;31(1):108–118. doi: 10.1128/aem.31.1.108-118.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]