Abstract
An investigation was conducted to determine the processes involved in the contamination of shellfish by viruses. Results of binding-release studies show that the process involves the attachment of viruses to mucus secreted, and then ingested, by shellfish during feeding. Analysis of the mucus-virus bond involved selective degradation of the mucus and use of chemical agents to block carboxyl and sulfate groups on the mucus. Results obtained indicate that the attachment of virus to mucus is primarily ionic and involves the binding of viral particles to sulfate radicals on the mucopolysaccharide moiety of shellfish mucus.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Di Girolamo R., Liston J., Matches J. Uptake and elimination of poliovirus by West Coast oysters. Appl Microbiol. 1975 Feb;29(2):260–264. doi: 10.1128/am.29.2.260-264.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HSIUNG G. D., MELNICK J. L. Plaque formation with poliomyelitis, Coxsackie, and orphan (echo) viruses in bottle cultures of monkey epithelial cells. Virology. 1955 Dec;1(5):533–535. doi: 10.1016/0042-6822(55)90041-6. [DOI] [PubMed] [Google Scholar]
- Hamblet F. E., Hill W. F., Akin E. W. Effect of plaque assay diluent upon enumeration of poliovirus type 1. Appl Microbiol. 1967 Jan;15(1):208–208. doi: 10.1128/am.15.1.208-.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liu O. C., Seraichekas H. R., Murphy B. L. Fate of poliovirus in northern quahaugs. Proc Soc Exp Biol Med. 1966 Feb;121(2):601–607. doi: 10.3181/00379727-121-30841. [DOI] [PubMed] [Google Scholar]
- PIOCH W. Uber die Darstellung saurer Mucopolysaccharide mit dem Kupferphthalocyaninfarbstoff Astrablau. Virchows Arch Pathol Anat Physiol Klin Med. 1957;330(3):337–346. doi: 10.1007/BF00954964. [DOI] [PubMed] [Google Scholar]
- SCOTT J. E. The solubility of cetylpyridinium complexes of biological polyanions in solution of salts. Biochim Biophys Acta. 1955 Nov;18(3):428–429. doi: 10.1016/0006-3002(55)90108-6. [DOI] [PubMed] [Google Scholar]
- Sobsey M. D., Wallis C., Melnick J. L. Development of a simple method for concentrating enteroviruses from oysters. Appl Microbiol. 1975 Jan;29(1):21–26. doi: 10.1128/am.29.1.21-26.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]