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. 1977 Feb;33(2):240–245. doi: 10.1128/aem.33.2.240-245.1977

Confirmation of the single-step membrane filtration procedure for estimating Pseudomonas aeruginosa densities in water.

B J Dutka, K K Kwan
PMCID: PMC170671  PMID: 403858

Abstract

The efficiency of two procedures, membrane filtration and most probable number, to resuscitate and enumerate Pseudomonas aeruginosa have been compared at two temperatures and varying incubation periods. Data indicate that the membrane filtration procedure using mPA or mPA medium B is more efficient than the most-probable-number procedure in estimating P. aeruginosa populations. It was also found that the specificity of the membrane filtration procedure was such that 92 to 99% of the colonies counted as P. aeruginosa were confirmed, whereas only 2.7 to 10% of the nontypical colonies were confirmed as P. aeruginosa. Furthermore, the data indicate that mPA medium B combined with a 3- to 4-day incubation period at 4.15 degrees C is slightly more specific than mPA medium and is a valid single-step procedure for the resuscitation and enumeration of P. aeruginosa from water or sewage effluent.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUHLMANN X., VISCHER W. A., BRUHIN H. Identification of apyocyanogenic strains of Pseudomonas aeruginosa. J Bacteriol. 1961 Nov;82:787–788. doi: 10.1128/jb.82.5.787-788.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brodsky M. H., Nixon M. C. Rapid method for detection of Pseudomonas aeruginosa on MacConkey agar under ultraviolet light. Appl Microbiol. 1973 Aug;26(2):219–220. doi: 10.1128/am.26.2.219-220.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown M. R., Foster J. H. A simple diagnostic milk medium for Pseudomonas aeruginosa. J Clin Pathol. 1970 Mar;23(2):172–177. doi: 10.1136/jcp.23.2.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Drake C. H. Evaluation of culture media for the isolation and enumeration of Pseudomonas aeruginosa. Health Lab Sci. 1966 Jan;3(1):10–19. [PubMed] [Google Scholar]
  5. Foster D. H., Hanes N. B., Lord S. M., Jr A critical examination of bathing water quality standards. J Water Pollut Control Fed. 1971 Nov;43(11):2229–2241. [PubMed] [Google Scholar]
  6. KING E. O., WARD M. K., RANEY D. E. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954 Aug;44(2):301–307. [PubMed] [Google Scholar]
  7. Levin M. A., Cabelli V. J. Membrane filter technique for enumeration of Pseudomonas aeruginosa. Appl Microbiol. 1972 Dec;24(6):864–870. doi: 10.1128/am.24.6.864-870.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McTernan W. F., Adams J. C., Rechard P. A. Comparison of methods for enumerating fluorescent bacteria. Appl Microbiol. 1974 Jan;27(1):290–291. doi: 10.1128/am.27.1.290-291.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. STEVENSON A. H. Studies of bathing water quality and health. Am J Public Health Nations Health. 1953 May;43(5 Pt 1):529–538. doi: 10.2105/ajph.43.5_pt_1.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sneath P. H., Collins V. G. A study in test reproducibility between laboratories: report of a Pseudomonas Working Party. Antonie Van Leeuwenhoek. 1974;40(4):481–527. doi: 10.1007/BF00403815. [DOI] [PubMed] [Google Scholar]

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