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. 1981 Feb;37(2):755–758. doi: 10.1128/jvi.37.2.755-758.1981

Convenient assay for interferons.

S Rubinstein, P C Familletti, S Pestka
PMCID: PMC171063  PMID: 6163873

Abstract

A convenient assay for interferons based on reduction of cytopathic effect was developed. The number of manipulations and the lengths of the various incubation steps were reduced to a minimum. The assay is simple to perform and can be completed within 16 h. Moreover, it can be used with various types of cells and a variety of viruses.

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Selected References

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  1. Armstrong J. A. Semi-micro, dye-binding assay for rabbit interferon. Appl Microbiol. 1971 Apr;21(4):723–725. doi: 10.1128/am.21.4.723-725.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Green J. A., Stanton G. J., Goode J., Baron S. Vesicular stomatitis virus plaque production in monolayer cultures with liquid overlay medium: description and adaptation to a one-day, human interferon-plaque. J Clin Microbiol. 1976 Dec;4(6):479–485. doi: 10.1128/jcm.4.6.479-485.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Jordan G. W. Quantitative aspects of interferon-induced plaque reduction: kinetics of interferon action. Virology. 1972 May;48(2):425–432. doi: 10.1016/0042-6822(72)90053-0. [DOI] [PubMed] [Google Scholar]
  4. LINDENMANN J., GIFFORD G. E. Studies on vaccinia virus plaque formation and its inhibition by interferon. III. A simplified plaque inhibition assay of interferon. Virology. 1963 Mar;19:302–309. doi: 10.1016/0042-6822(63)90068-0. [DOI] [PubMed] [Google Scholar]
  5. MADIN S. H., DARBY N. B., Jr Established kidney cell lines of normal adult bovine and ovine origin. Proc Soc Exp Biol Med. 1958 Jul;98(3):574–576. doi: 10.3181/00379727-98-24111. [DOI] [PubMed] [Google Scholar]
  6. Richmond J. Y., Polatnick J., Knudsen R. C. Microassay for interferon, using [3H]uridine, microculture plates, and a multiple automated sample harvester. Appl Environ Microbiol. 1980 Apr;39(4):823–827. doi: 10.1128/aem.39.4.823-827.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rubinstein M., Rubinstein S., Familletti P. C., Miller R. S., Waldman A. A., Pestka S. Human leukocyte interferon: production, purification to homogeneity, and initial characterization. Proc Natl Acad Sci U S A. 1979 Feb;76(2):640–644. doi: 10.1073/pnas.76.2.640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sedmak J. J., Grossberg S. E., Jameson P. The neuraminidase yield-reduction bioassay of human other interferons. Proc Soc Exp Biol Med. 1975 Jun;149(2):433–438. doi: 10.3181/00379727-149-38822. [DOI] [PubMed] [Google Scholar]
  9. Vilcek J., Havell E. A. Stabilization of interferon messenger RNA activity by treatment of cells with metabolic inhibitors and lowering of the incubation temperature. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3909–3913. doi: 10.1073/pnas.70.12.3909. [DOI] [PMC free article] [PubMed] [Google Scholar]

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