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. 1981 Feb;37(2):784–794. doi: 10.1128/jvi.37.2.784-794.1981

Structure and restriction enzyme maps of the circularly permuted DNA of staphylococcal bacteriophage phi 11.

S Löfdahl, J Zabielski, L Philipson
PMCID: PMC171066  PMID: 6261018

Abstract

One restriction enzyme map of Staphylococcus aureus bacteriophage phi 11 DNA was established by reciprocal double digestions with the enzymes EcoRI, HaeII, and KpnI. The sequential order of the EcoRI fragments was thereafter established by a novel approach involving blotting of DNA partially cleaved with EcoRI and the probing the blots with nick-translated terminal fragments. A circular map of the phi 11 DNA was established, and the phage genome was circularly permuted based on the failure to end label mature viral DNA, restriction maps of replicating DNA, and finally, homoduplex analysis in the electron microscope. A restriction enzyme map of the prophage form of phi 11 DNA was obtained by analysis of chromosomal DNA from a lysogenic strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akusjärvi G., Pettersson J. Sequence analysis of adenovirus DNA. IV. The genomic sequences encoding the common tripartite leader of late adenovirus messenger RNA. J Mol Biol. 1979 Oct 15;134(1):143–158. doi: 10.1016/0022-2836(79)90417-0. [DOI] [PubMed] [Google Scholar]
  2. Botchan M., Topp W., Sambrook J. The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell. 1976 Oct;9(2):269–287. doi: 10.1016/0092-8674(76)90118-5. [DOI] [PubMed] [Google Scholar]
  3. Brown D. T., Brown N. C., Burlingham B. T. Morphology and physical properties of Staphylococcus bacteriophage P11-M15. J Virol. 1972 Apr;9(4):664–671. doi: 10.1128/jvi.9.4.664-671.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kretschmer P. J., Egan J. B. Genetic map of the Staphylococcal bacteriophage phi11. J Virol. 1975 Sep;16(3):642–651. doi: 10.1128/jvi.16.3.642-651.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lee C. S., Davis R. W., Davidson N. A physical study by electron microscopy of the terminally reptitious, circularly permuted DNA from the coliphage particles of Escherichia coli 15. J Mol Biol. 1970 Feb 28;48(1):1–22. doi: 10.1016/0022-2836(70)90215-9. [DOI] [PubMed] [Google Scholar]
  6. Löfdahl S., Sjöström J. E., Philipson L. Characterization of small plasmids from Staphylococcus aureus. Gene. 1978 Apr;3(2):145–159. [PubMed] [Google Scholar]
  7. Löfdahl S., Sjöström J. E., Philipson L. Cloning of restriction fragments of DNA from staphylococcal bacteriophage phi 11. J Virol. 1981 Feb;37(2):795–801. doi: 10.1128/jvi.37.2.795-801.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MacHattie L. A., Ritchie D. A., Thomas C. A., Jr, Richardson C. C. Terminal repetition in permuted T2 bacteriophage DNA molecules. J Mol Biol. 1967 Feb 14;23(3):355–363. doi: 10.1016/s0022-2836(67)80110-4. [DOI] [PubMed] [Google Scholar]
  9. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. NOVICK R. P. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. doi: 10.1099/00221287-33-1-121. [DOI] [PubMed] [Google Scholar]
  11. Novick R. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology. 1967 Sep;33(1):155–166. doi: 10.1016/0042-6822(67)90105-5. [DOI] [PubMed] [Google Scholar]
  12. Rhoades M., Thomas C. A., Jr The P22 bacteriophage DNA molecule. II. Circular intracellular forms. J Mol Biol. 1968 Oct 14;37(1):41–61. doi: 10.1016/0022-2836(68)90072-7. [DOI] [PubMed] [Google Scholar]
  13. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  14. Sjöström J. E., Lindberg M., Philipson L. Competence for transfection in Staphylococcus aureus. J Bacteriol. 1973 Feb;113(2):576–585. doi: 10.1128/jb.113.2.576-585.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sjöström J. E., Philipson L. Role of the phi 11 phage genome in competence of Staphylococcus aureus. J Bacteriol. 1974 Jul;119(1):19–32. doi: 10.1128/jb.119.1.19-32.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith H. O., Birnstiel M. L. A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976 Sep;3(9):2387–2398. doi: 10.1093/nar/3.9.2387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  18. THOMAS C. A., Jr, MACHATTIE L. A. CIRCULAR T2 DNA MOLECULES. Proc Natl Acad Sci U S A. 1964 Nov;52:1297–1301. doi: 10.1073/pnas.52.5.1297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Thompson N. E., Pattee P. A. Transformation in Staphylococcus aureus: role of bacteriophage and incidence of competence among strains. J Bacteriol. 1977 Feb;129(2):778–788. doi: 10.1128/jb.129.2.778-788.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tibbetts C., Johansson K., Philipson L. Hydroxyapatite chromatography and formamide denaturation of adenovirus DNA. J Virol. 1973 Aug;12(2):218–225. doi: 10.1128/jvi.12.2.218-225.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tye B. K., Chan R. K., Botstein D. Packaging of an oversize transducing genome by Salmonella phage P22. J Mol Biol. 1974 Jan 5;85(4):485–500. doi: 10.1016/0022-2836(74)90311-8. [DOI] [PubMed] [Google Scholar]
  22. Tye B. K., Huberman J. A., Botstein D. Non-random circular permutation of phage P22 DNA. J Mol Biol. 1974 Jan 5;85(4):501–528. doi: 10.1016/0022-2836(74)90312-x. [DOI] [PubMed] [Google Scholar]

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