Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1981 Mar;37(3):916–921. doi: 10.1128/jvi.37.3.916-921.1981

Colicin activity and abortive infection of T5 bacteriophage in Escherichia coli (ColIb).

D H Duckworth, G B Dunn, T Pinkerton, K Rose, S K Walia
PMCID: PMC171088  PMID: 6262536

Abstract

We performed three types of experiments to test the hypothesis that abortive infection of T5 bacteriophage in Escherichia coli (ColIb+) is due to internally released colicin. (i) We measured the sensitivity of cells to colicin under a variety of conditions and then looked at the plating efficiency of T5 in ColIb+ cells under these same conditions. Cells grown at 42 degrees C or with hexanol had a reduced sensitivity to externally added colicin and an increased efficiency for T5 when the ColIb plasmid was present in the infected cells. Phage growth was far from normal, however. (ii) We measured the colicin sensitivity of a mutant bacterium that grew T5 normally even in the presence of the ColIb plasmid and measured the plating efficiency of T5 on another mutant that was colicin tolerant. Here again, the correlation between colicin activity and inhibition of phage replication was not complete. (iii) We looked for colicin-negative plasmid mutants and tested the ability of cells containing these plasmids to support the growth of T5. These experiments used Tn5, a kanamycin resistance transposon, as the mutagen. All possible combinations of colicin production and phage inhibition were found, including mutants that produced no colicin but still inhibited phage production.

Full text

PDF
918

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cardelli J., Konisky J. Isolation and characterization of an Escherichia coli mutant tolerant to colicins Ia and Ib. J Bacteriol. 1974 Aug;119(2):379–385. doi: 10.1128/jb.119.2.379-385.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cheung A. K., Duckworth D. H. Membrane damage in abortive infections of colicin Ib-containing Escherichia coli by bacteriophage T5. J Virol. 1977 Jul;23(1):98–105. doi: 10.1128/jvi.23.1.98-105.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Glenn J., Duckworth D. H. Amino acid and sugar transport in Escherichia coli (ColIb) during abortive infection by bacteriophage T5. J Virol. 1979 May;30(2):421–430. doi: 10.1128/jvi.30.2.421-430.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Glenn J., Duckworth D. H. Fluorescence changes of a membrane-bound dye during bacteriophage T5 infection of Escherichia coli. J Virol. 1980 Jan;33(1):553–556. doi: 10.1128/jvi.33.1.553-556.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Glenn J., Duckworth D. H. Ion fluxes during T5 bacteriophage infection of Escherichia coli. Arch Biochem Biophys. 1980 May;201(2):576–585. doi: 10.1016/0003-9861(80)90547-0. [DOI] [PubMed] [Google Scholar]
  6. Hull R., Moody E. E. Isolation and genetic characterizaion of Escherichia coli K-12 mutations affecting bacteriophage T5 restriction by the ColIb plasmid. J Bacteriol. 1976 Jul;127(1):229–236. doi: 10.1128/jb.127.1.229-236.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ingram L. O. Adaptation of membrane lipids to alcohols. J Bacteriol. 1976 Feb;125(2):670–678. doi: 10.1128/jb.125.2.670-678.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ingram L. O., Vreeland N. S. Differential effects of ethanol and hexanol on the Escherichia coli cell envelope. J Bacteriol. 1980 Nov;144(2):481–488. doi: 10.1128/jb.144.2.481-488.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ito K., Sato T., Yura T. Synthesis and assembly of the membrane proteins in E. coli. Cell. 1977 Jul;11(3):551–559. doi: 10.1016/0092-8674(77)90073-3. [DOI] [PubMed] [Google Scholar]
  10. Jorgensen R. A., Rothstein S. J., Reznikoff W. S. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol Gen Genet. 1979;177(1):65–72. doi: 10.1007/BF00267254. [DOI] [PubMed] [Google Scholar]
  11. Ladbrooke B. D., Chapman D. Thermal analysis of lipids, proteins and biological membranes. A review and summary of some recent studies. Chem Phys Lipids. 1969 Dec;3(4):304–356. doi: 10.1016/0009-3084(69)90040-1. [DOI] [PubMed] [Google Scholar]
  12. Lanni Y. T. First-step-transfer deoxyribonucleic acid of bacteriophage T5. Bacteriol Rev. 1968 Sep;32(3):227–242. doi: 10.1128/br.32.3.227-242.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Marr A. G., Ingraham J. L. EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI. J Bacteriol. 1962 Dec;84(6):1260–1267. doi: 10.1128/jb.84.6.1260-1267.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McCorquodale D. J., Shaw A. R., Moody E. E., Hull R. A., Morgan A. F. Is abortive infection by bacteriophage BF23 of Escherichia coli harboring ColIb plasmids due to cell killing by internally liberated colicin Ib? J Virol. 1979 Jul;31(1):31–41. doi: 10.1128/jvi.31.1.31-41.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McCorquodale D. J. The T-odd bacteriophages. CRC Crit Rev Microbiol. 1975 Dec;4(2):101–159. doi: 10.3109/10408417509111574. [DOI] [PubMed] [Google Scholar]
  16. Morrison T. G., Malamy M. H. T7 translational control mechanisms and their inhibiton by F factors. Nat New Biol. 1971 May 12;231(19):37–41. doi: 10.1038/newbio231037a0. [DOI] [PubMed] [Google Scholar]
  17. Nisioka T., Ozeki H. Early abortive lysis by phage BF23 in Escherichia coli K-12 carrying the colicin Ib factor. J Virol. 1968 Nov;2(11):1249–1254. doi: 10.1128/jvi.2.11.1249-1254.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rogers S. G., Godwin E. A., Shinosky E. S., Rhoades M. Interruption-deficient mutants of bacteriophage T5 I. Isolation and general properties. J Virol. 1979 Feb;29(2):716–725. doi: 10.1128/jvi.29.2.716-725.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sekiguchi M. Studies on the physiological defect in rII mutants of bacteriophage T4. J Mol Biol. 1966 Apr;16(2):503–522. doi: 10.1016/s0022-2836(66)80188-2. [DOI] [PubMed] [Google Scholar]
  20. Strobel M., Nomura M. Restriction of the growth of bacteriophage BF23 by a colicine I (Col I-P9) factor. Virology. 1966 Apr;28(4):763–765. doi: 10.1016/0042-6822(66)90263-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES