Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1981 Apr;38(1):104–114. doi: 10.1128/jvi.38.1.104-114.1981

Map of restriction sites on bacteriophage T4 cytosine-containing DNA for endonucleases bamHI, BglII, KpnI, PvuI, SalI, and XbaI.

R C Marsh, M L Hepburn
PMCID: PMC171130  PMID: 6264096

Abstract

A complete map of the cleavage sites of restriction endonucleases BamHI, BglII, KpnI, PvuI, SalI, and XbaI was determined for the cytosine-containing DNA of a bacteriophage T4 alc mutant. The 56 sequence-specific sites were assigned map coordinates based on a least-squares analysis of measured fragment lengths. Altogether, the lengths of 118 fragments from single and double enzyme digestions were measured by electrophoresis of the fragments in agarose gels. DNA fragments of known sequence or DNA fragments calibrated with fragments of known sequence were used as standards. The greatest deviation between an experimentally measured fragment length and its computed map coordinates was 3.0%; the average deviation was 0.8%. The total length of the wild-type T4 genome was calculated to be 166,200 base pairs.

Full text

PDF
108

Images in this article

106Image
on p.106
109Image
on p.109
109Image
on p.109
111Image
on p.111
112Image
on p.112

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blin N., von Gabain A., Bujard H. Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes. FEBS Lett. 1975 Apr 15;53(1):84–86. doi: 10.1016/0014-5793(75)80688-0. [DOI] [PubMed] [Google Scholar]
  2. Carlson K. Correlation between genetic map and map of cleavage sites for sequence-specific endonucleases SalI, KpnI, BglI, and BamHI in bacteriophage T4 cytosine-containing DNA. J Virol. 1980 Oct;36(1):1–17. doi: 10.1128/jvi.36.1.1-17.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carlson K., Nicolaisen B. Cleavage map of bacteriophage T4 cytosine-containing DNA by sequence-specific endonucleases SalI and KpnI. J Virol. 1979 Jul;31(1):112–123. doi: 10.1128/jvi.31.1.112-123.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Daniels D. L., de Wet J. R., Blattner F. R. New map of bacteriophage lambda DNA. J Virol. 1980 Jan;33(1):390–400. doi: 10.1128/jvi.33.1.390-400.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Georgopoulos C. P., Revel H. R. Studies with glucosyl transferase mutants of the T-even bacteriophages. Virology. 1971 May;44(2):271–285. doi: 10.1016/0042-6822(71)90259-5. [DOI] [PubMed] [Google Scholar]
  6. Kaplan D. A., Nierlich D. P. Cleavage of Nonglucosylated Bacteriophage T4 deoxyribonucleic acid by Restriction Endonuclease Eco RI. J Biol Chem. 1975 Mar 25;250(6):2395–2397. [PubMed] [Google Scholar]
  7. Kiko H., Niggemann E., Rüger W. Physical mapping of the restriction fragments obtained from bacteriophage T4 dC-DNA with the restriction endonucleases SmaI, KpnI and BglII. Mol Gen Genet. 1979;172(3):303–312. doi: 10.1007/BF00271730. [DOI] [PubMed] [Google Scholar]
  8. Kim J. S., Davidson N. Electron microscope heteroduplex study of sequence relations of T2, T4, and T6 bacteriophage DNAs. Virology. 1974 Jan;57(1):93–111. doi: 10.1016/0042-6822(74)90111-1. [DOI] [PubMed] [Google Scholar]
  9. Kutter E., Beug A., Sluss R., Jensen L., Bradley D. The production of undegraded cytosine-containing DNA by bacteriophage T4 in the absence of dCTPase and endonucleases II and IV, and its effects on T4-directed protein synthesis. J Mol Biol. 1975 Dec 25;99(4):591–607. doi: 10.1016/s0022-2836(75)80174-4. [DOI] [PubMed] [Google Scholar]
  10. Lawn R. M., Fritsch E. F., Parker R. C., Blake G., Maniatis T. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA. Cell. 1978 Dec;15(4):1157–1174. doi: 10.1016/0092-8674(78)90043-0. [DOI] [PubMed] [Google Scholar]
  11. Mosig G., Carnighan J. R., Bibring J. B., Cole R., Bock H. G., Bock S. Coordinate variation in lengths of deoxyribonucleic acid molecules and head lengths in morphological variants of bacteriophage T4. J Virol. 1972 May;9(5):857–871. doi: 10.1128/jvi.9.5.857-871.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. O'Farrell P. H., Kutter E., Nakanishi M. A restriction map of the bacteriophage T4 genome. Mol Gen Genet. 1980;179(2):421–435. doi: 10.1007/BF00425473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. O'Farrell P. Z., Gold L. M., Huang W. M. The identification of prereplicative bacteriophage T4 proteins. J Biol Chem. 1973 Aug 10;248(15):5499–5501. [PubMed] [Google Scholar]
  14. Rüger W., Neumann M., Rohr U., Niggemann E. The complete maps of BglII, SalI and XhoI restriction sites on T4 dC-DNA. Mol Gen Genet. 1979 Nov;176(3):417–425. doi: 10.1007/BF00333106. [DOI] [PubMed] [Google Scholar]
  15. Schroeder J. L., Blattner F. R. Least-squares method for restriction mapping. Gene. 1978 Oct;4(2):167–174. doi: 10.1016/0378-1119(78)90028-8. [DOI] [PubMed] [Google Scholar]
  16. Snyder L., Gold L., Kutter E. A gene of bacteriophage T4 whose product prevents true late transcription on cytosine-containing T4 DNA. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3098–3102. doi: 10.1073/pnas.73.9.3098. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Streisinger G., Emrich J., Stahl M. M. Chromosome structure in phage t4, iii. Terminal redundancy and length determination. Proc Natl Acad Sci U S A. 1967 Feb;57(2):292–295. doi: 10.1073/pnas.57.2.292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sugden B., De Troy B., Roberts R. J., Sambrook J. Agarose slab-gel electrophoresis equipment. Anal Biochem. 1975 Sep;68(1):36–46. doi: 10.1016/0003-2697(75)90676-4. [DOI] [PubMed] [Google Scholar]
  19. THOMAS C. A., Jr, MACHATTIE L. A. CIRCULAR T2 DNA MOLECULES. Proc Natl Acad Sci U S A. 1964 Nov;52:1297–1301. doi: 10.1073/pnas.52.5.1297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Takahashi H., Shimizu M., Saito H., Ikeda Y. Studies of viable T4 bacteriophage containing cytosine-substituted DNA (T4dC phage). II. Cleavage of T4dC DNA by endonuclease SalI and bam HI. Mol Gen Genet. 1979 Jan 5;168(1):49–53. doi: 10.1007/BF00267932. [DOI] [PubMed] [Google Scholar]
  21. Wilson G. G., Neve R. L., Edlin G. J., Konigsberg W. H. The BAM H1 restriction site in the bacteriophage T4 chromosome is located in or near gene 8. Genetics. 1979 Oct;93(2):285–296. doi: 10.1093/genetics/93.2.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wood W. B., Revel H. R. The genome of bacteriophage T4. Bacteriol Rev. 1976 Dec;40(4):847–868. doi: 10.1128/br.40.4.847-868.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yee J. K., Marsh R. C. Alignment of a restriction map with the genetic map of bacteriophage T4. J Virol. 1981 Apr;38(1):115–124. doi: 10.1128/jvi.38.1.115-124.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES