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. 1981 Apr;38(1):347–355. doi: 10.1128/jvi.38.1.347-355.1981

Mutants of Fujinami sarcoma virus which are temperature sensitive for cellular transformation and protein kinase activity.

T Hanafusa, B Mathey-Prevot, R A Feldman, H Hanafusa
PMCID: PMC171157  PMID: 6264108

Abstract

Two temperature-sensitive mutants of Fujinami sarcoma virus were isolated and characterized. Cells infected with the mutants were temperature sensitive in focus formation, colony formation, increased sugar uptake, and synthesis of plasminogen activator. The changes between transformed and nontransformed states of cultures were completely reversible by shifting the temperature. A Fujinami sarcoma virus-specific protein of 130,000 daltons, p130, was synthesized in mutant-infected cells regardless of the temperature, but the immunoprecipitates of p130 from extracts of infected cells were active in protein kinase only when cells had been incubated at the permissive temperature. These results appear to indicate that p130 is the transforming protein of Fujinami sarcoma virus, and that its protein kinase activity plays a crucial role in cell transformation by this virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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