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. 1981 May;38(2):792–796. doi: 10.1128/jvi.38.2.792-796.1981

Vesicular stomatitis virus RNA polymerase can read through the boundary between the leader and N genes in vitro.

R C Herman, R A Lazzarini
PMCID: PMC171211  PMID: 6264146

Abstract

Triphosphorylated in vitro transcripts of vesicular stomatitis virus were selected by mercury-Sepharose chromatography using adenosine-5'-O-(3-thiotriphosphate) as an affinity probe. Numerous RNAs ranging from less than 47 up to several hundred nucleotides in length were detected. Some of these contain the leader RNA covalently linked to transcripts of the N gene. A comparison of the published genomic sequences at the estimated termination sites of several of these RNAs reveals some homology with the sequence present at both the end of the leader and polymerase genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ball L. A., White C. N. Order of transcription of genes of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1976 Feb;73(2):442–446. doi: 10.1073/pnas.73.2.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carroll A. R., Wagner R. R. Adenosine-5'-O-(3-thiotriphosphate) as an affinity probe for studying leader RNA's transcribed by vesicular stomatitis virus. J Biol Chem. 1979 Oct 10;254(19):9339–9341. [PubMed] [Google Scholar]
  4. Colonno R. J., Banerjee A. K. A unique RNA species involved in initiation of vesicular stomatitis virus RNA transcription in vitro. Cell. 1976 Jun;8(2):197–204. doi: 10.1016/0092-8674(76)90003-9. [DOI] [PubMed] [Google Scholar]
  5. Colonno R. J., Banerjee A. K. Complete nucleotide sequence of the leader RNA synthesized in vitro by vesicular stomatitis virus. Cell. 1978 Sep;15(1):93–101. doi: 10.1016/0092-8674(78)90085-5. [DOI] [PubMed] [Google Scholar]
  6. England T. E., Uhlenbeck O. C. 3'-terminal labelling of RNA with T4 RNA ligase. Nature. 1978 Oct 12;275(5680):560–561. doi: 10.1038/275560a0. [DOI] [PubMed] [Google Scholar]
  7. Hefti E., Bishop D. H. The 5' sequences of VSV in vitro transcription product RNA (+/-SAM). Biochem Biophys Res Commun. 1976 Jan 26;68(2):393–400. doi: 10.1016/0006-291x(76)91158-x. [DOI] [PubMed] [Google Scholar]
  8. Keene J. D., Schubert M., Lazzarini R. A., Rosenberg M. Nucleotide sequence homology at the 3' termini of RNA from vesicular stomatitis virus and its defective interfering particles. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3225–3229. doi: 10.1073/pnas.75.7.3225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McGeoch D. J., Dolan A. Sequence of 200 nucleotides at the 3'-terminus of the genome RNA of vesicular stomatitis virus. Nucleic Acids Res. 1979 Jul 25;6(10):3199–3211. doi: 10.1093/nar/6.10.3199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moyer S. A., Banerjee A. K. Messenger RNA species synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus. Cell. 1975 Jan;4(1):37–43. doi: 10.1016/0092-8674(75)90131-2. [DOI] [PubMed] [Google Scholar]
  11. Peacock A. C., Dingman C. W. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. Biochemistry. 1967 Jun;6(6):1818–1827. doi: 10.1021/bi00858a033. [DOI] [PubMed] [Google Scholar]
  12. Petric M., Prevec L. Vesicular stomatitis virus--a new interfering particle, intracellular structures, and virus-specific RNA. Virology. 1970 Aug;41(4):615–630. doi: 10.1016/0042-6822(70)90427-7. [DOI] [PubMed] [Google Scholar]
  13. Rose J. K. Complete intergenic and flanking gene sequences from the genome of vesicular stomatitis virus. Cell. 1980 Feb;19(2):415–421. doi: 10.1016/0092-8674(80)90515-2. [DOI] [PubMed] [Google Scholar]
  14. Schubert M., Keene J. D., Herman R. C., Lazzarini R. A. Site on the vesicular stomatitis virus genome specifying polyadenylation and the end of the L gene mRNA. J Virol. 1980 May;34(2):550–559. doi: 10.1128/jvi.34.2.550-559.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith M. M., Reeve A. E., Huang R. C. Analysis of RNA initiated in isolated mouse myeloma nuclei using purine nucleoside 5'[gamma-S]triphosphates as affinity probes. Cell. 1978 Oct;15(2):615–626. doi: 10.1016/0092-8674(78)90030-2. [DOI] [PubMed] [Google Scholar]
  16. Testa D., Banerjee A. K. In vitro synthesis of a possible precursor RNA by purified vesicular stomatitis virus. Biochem Biophys Res Commun. 1978 May 30;82(2):655–664. doi: 10.1016/0006-291x(78)90925-7. [DOI] [PubMed] [Google Scholar]
  17. Testa D., Chanda P. K., Banerjee A. K. In vitro synthesis of the full-length complement of the negative-strand genome RNA of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1980 Jan;77(1):294–298. doi: 10.1073/pnas.77.1.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Testa D., Chanda P. K., Banerjee A. K. Unique mode of transcription in vitro by Vesicular stomatitis virus. Cell. 1980 Aug;21(1):267–275. doi: 10.1016/0092-8674(80)90134-8. [DOI] [PubMed] [Google Scholar]

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