Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1970 May;6(5):767–772.

An immunofluorescence mixed staining technique for the detection of IgG-rheumatoid factor and IgG-β1C complexes in tissues

A Tursi, D Trizio, L Bonomo
PMCID: PMC1712716  PMID: 4097831

Abstract

A mixed immunofluorescent staining method for the detection of immune complexes in the tissues is described.

This method demonstrated the presence of IgG-rheumatoid factor and IgG-β1c complexes in synovial membrane from patients with rheumatoid arthritis.

Full text

PDF
772

Images in this article

770-1Fig. 1
on p.770-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andres G. A., Accinni L., Hsu K. C., Zabriskie J. B., Seegal B. C. Electron microscopic studies of human glomerulonephritis with ferritin-conjugated antibody. Localization of antigen-antibody complexes in glomerular structures of patients with acute glomerulonephritis. J Exp Med. 1966 Feb 1;123(2):399–412. doi: 10.1084/jem.123.2.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BUTLER V. P., Jr, VAUGHAN J. H. HEMAGGLUTINATION BY RHEUMATOID FACTOR OF CELLS COATED WITH ANIMAL GAMMA GLOBULINS. Proc Soc Exp Biol Med. 1964 Jul;116:585–593. doi: 10.3181/00379727-116-29313. [DOI] [PubMed] [Google Scholar]
  3. Bonomo L., Tursi A., Gillardi U. Distribution of the anti-gamma globulin factors in the synovial membrane and other tissues in various diseases. Ann Rheum Dis. 1968 Mar;27(2):122–129. doi: 10.1136/ard.27.2.122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cebra J. J., Goldstein G. Chromatographic purification of tetramethylrhodamine-immune globulin conjugates and their use in the cellular localization of rabbit gamma-globulin polypeptide chains. J Immunol. 1965 Aug;95(2):230–245. [PubMed] [Google Scholar]
  5. DIXON F. J., FELDMAN J. D., VAZQUEZ J. J. Experimental glomerulonephritis. The pathogenesis of a laboratory model resembling the spectrum of human glomerulonephritis. J Exp Med. 1961 May 1;113:899–920. doi: 10.1084/jem.113.5.899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. EDELMAN G. M., KUNKEL H. G., FRANKLIN E. C. Interaction of the rheumatoid factor with antigen-antibody complexes and aggregated gamma globulin. J Exp Med. 1958 Jul 1;108(1):105–120. doi: 10.1084/jem.108.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hijmans W., Schuit H. R., Klein F. An immunofluorescence procedure for the detection of intracellular immunoglobulins. Clin Exp Immunol. 1969 Apr;4(4):457–472. [PMC free article] [PubMed] [Google Scholar]
  8. KAPLAN M. H., BOLANDE R., RAKITA L., BLAIR J. PRESENCE OF BOUND IMMUNOGLOBULINS AND COMPLEMENT IN THE MYOCARDIUM IN ACUTE RHEUMATIC FEVER. ASSOCIATION WITH CARDIAC FAILURE. N Engl J Med. 1964 Sep 24;271:637–645. doi: 10.1056/NEJM196409242711301. [DOI] [PubMed] [Google Scholar]
  9. LACHMANN P. J., MULLER-EBERHARD H. J., KUNKEL H. G., PARONETTO F. The localization of in vivo bound complement in tissue section. J Exp Med. 1962 Jan 1;115:63–82. doi: 10.1084/jem.115.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McCormick J. N. An Immunofluorescence Study of Rheumatoid Factor. Ann Rheum Dis. 1963 Jan;22(1):1–10. doi: 10.1136/ard.22.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. RAWSON A. J., ABELSON N. M., HOLLANDER J. L. STUDIES ON THE PATHOGENESIS OF RHEUMATOID JOINT INFLAMMATION. II. INTRACYTOPLASMIC PARTICULATE COMPLEXES IN RHEUMATOID SYNOVIAL FLUIDS. Ann Intern Med. 1965 Feb;62:281–284. doi: 10.7326/0003-4819-62-2-281. [DOI] [PubMed] [Google Scholar]
  12. RIGGS J. L., SEIWALD R. J., BURCKHALTER J. H., DOWNS C. M., METCALF T. G. Isothiocyanate compounds as fluorescent labeling agents for immune serum. Am J Pathol. 1958 Nov-Dec;34(6):1081–1097. [PMC free article] [PubMed] [Google Scholar]
  13. Rodman W. S., Williams R. C., Jr, Bilka P. J., Müller-Eberhard H. J. Immunofluorescent localization of the third and the fourth component of complement in synovial tissue from patients with rheumatoid arthritis. J Lab Clin Med. 1967 Jan;69(1):141–150. [PubMed] [Google Scholar]
  14. Stratton F. Zymosan coated particles used to prepare anti-beta-1C. Vox Sang. 1966 Mar-Apr;11(2):232–237. doi: 10.1111/j.1423-0410.1966.tb04230.x. [DOI] [PubMed] [Google Scholar]
  15. Vaughan J. H., Barrnett E. V., Sobel M. V., Jacox R. F. Intracytoplasmic inclusions of immunoglobulins in rheumatoid arthritis and other diseases. Arthritis Rheum. 1968 Apr;11(2):125–134. doi: 10.1002/art.1780110202. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES