Abstract
A microtechnique is described to quantitate in vitro transformation of lymphocytes from man and marmosets following stimulation by PHA and different antigens. The 14C-thymidine uptake of lymphocytes grown in cultures of 0·06–0·2 ml of whole blood was measured by liquid scintillation. The magnitude, consistency and reproducibility of the results were similar to those achieved using cultures of purified lymphocytes or cell-rich plasma but fewer technical procedures were required.
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Selected References
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- Deinhardt F., Shramek G. J. Immunization against mumps. Prog Med Virol. 1969;11:126–153. [PubMed] [Google Scholar]
- Hartog M., Cline M. J., Grodsky G. M. The response of human leucocyte cultures to stimulation by tuberculin and phytohaemagglutinin measured by the uptake of radioactive thymidine. Clin Exp Immunol. 1967 Mar;2(2):217–228. [PMC free article] [PubMed] [Google Scholar]
- Heitmann H. J. Vereinfachte Methodik der Lymphocytenkultur als Allergie-Test. Mikro-Vollblutmethode. Hautarzt. 1967 Oct;18(10):459–461. [PubMed] [Google Scholar]
- Knight S., Ling N. R. A comparison of the responses of lymphocytes from blood, spleen, thymus and appendix of rabbits of different ages to stimulation "in vitro" with staphylococcal filtrate. Clin Exp Immunol. 1969 Jun;4(6):667–684. [PMC free article] [PubMed] [Google Scholar]
- Moore G. E., Gerner R. E., Franklin H. A. Culture of normal human leukocytes. JAMA. 1967 Feb 20;199(8):519–524. [PubMed] [Google Scholar]
- Wilson J. D., Thomson A. E. Death and division of lymphocytes. Neglected factors in assessment of P.H.A.-induced transformation. Lancet. 1968 Nov 23;2(7578):1120–1123. doi: 10.1016/s0140-6736(68)91583-3. [DOI] [PubMed] [Google Scholar]