Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1981 Jul;39(1):290–294. doi: 10.1128/jvi.39.1.290-294.1981

Transfection of KB Cells by Liposomes Containing Adenovirus Type 2 DNA

Stephen E Straus 1, Tazewell Wilson 2, Heschel J Raskas 3
PMCID: PMC171288  PMID: 7277577

Abstract

Adenovirus type 2 DNA was entrapped in liposomes which were then used to transfect KB cells with an efficiency of ∼4,000 plaques per μg of encapsulated DNA.

Full text

PDF
294

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chinnadurai G., Chinnadurai S., Green M. Enhanced infectivity of adenovirus type 2 DNA and a DNA-protein complex. J Virol. 1978 Apr;26(1):195–199. doi: 10.1128/jvi.26.1.195-199.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Craig E. A., Raskas H. J. Two classes of cytoplasmic viral RNA synthesized early in productive infection with adenovirus 2. J Virol. 1974 Oct;14(4):751–757. doi: 10.1128/jvi.14.4.751-757.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dimitriadis G. J. Entrapment of plasmid DNA in liposomes. Nucleic Acids Res. 1979 Jun 25;6(8):2697–2705. doi: 10.1093/nar/6.8.2697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dimitriadis G. J. Translation of rabbit globin mRNA introduced by liposomes into mouse lymphocytes. Nature. 1978 Aug 31;274(5674):923–924. doi: 10.1038/274923a0. [DOI] [PubMed] [Google Scholar]
  5. Fraley R. T., Fornari C. S., Kaplan S. Entrapment of a bacterial plasmid in phospholipid vesicles: potential for gene transfer. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3348–3352. doi: 10.1073/pnas.76.7.3348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fraley R., Subramani S., Berg P., Papahadjopoulos D. Introduction of liposome-encapsulated SV40 DNA into cells. J Biol Chem. 1980 Nov 10;255(21):10431–10435. [PubMed] [Google Scholar]
  7. Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. doi: 10.1016/0042-6822(73)90341-3. [DOI] [PubMed] [Google Scholar]
  8. Green M., Piña M., Kimes R. C. Biochemical studies on adenovirus multiplication. XII. Plaquing efficiencies of purified human adenoviruses. Virology. 1967 Mar;31(3):562–565. doi: 10.1016/0042-6822(67)90241-3. [DOI] [PubMed] [Google Scholar]
  9. Hoffman R. M., Margolis L. B., Bergelson L. D. Binding and entrapment of high molecular weight DNA by lecithin liposomes. FEBS Lett. 1978 Sep 15;93(2):365–368. doi: 10.1016/0014-5793(78)81141-7. [DOI] [PubMed] [Google Scholar]
  10. Liposomes and their uses in biology and medicine. Ann N Y Acad Sci. 1978;308:1–462. [PubMed] [Google Scholar]
  11. Lurquin P. F. Entrapment of plasmid DNA by liposomes and their interactions with plant protoplasts. Nucleic Acids Res. 1979 Aug 24;6(12):3773–3784. doi: 10.1093/nar/6.12.3773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mannino R. J., Allebach E. S., Strohl W. A. Encapsulation of high molecular weight DNA in large unilamellar phospholipid vesicles. Dependence on the size of the DNA. FEBS Lett. 1979 May 15;101(2):229–232. doi: 10.1016/0014-5793(79)81014-5. [DOI] [PubMed] [Google Scholar]
  13. Ostro M. J., Giacomoni D., Lavelle D., Paxton W., Dray S. Evidence for translation of rabbit globin mRNA after liposome-mediated insertion into a human cell line. Nature. 1978 Aug 31;274(5674):921–923. doi: 10.1038/274921a0. [DOI] [PubMed] [Google Scholar]
  14. Pagano R. E., Weinstein J. N. Interactions of liposomes with mammalian cells. Annu Rev Biophys Bioeng. 1978;7:435–468. doi: 10.1146/annurev.bb.07.060178.002251. [DOI] [PubMed] [Google Scholar]
  15. Papahadjopoulos D., Vail W. J., Jacobson K., Poste G. Cochleate lipid cylinders: formation by fusion of unilamellar lipid vesicles. Biochim Biophys Acta. 1975 Jul 3;394(3):483–491. doi: 10.1016/0005-2736(75)90299-0. [DOI] [PubMed] [Google Scholar]
  16. Portis A., Newton C., Pangborn W., Papahadjopoulos D. Studies on the mechanism of membrane fusion: evidence for an intermembrane Ca2+-phospholipid complex, synergism with Mg2+, and inhibition by spectrin. Biochemistry. 1979 Mar 6;18(5):780–790. doi: 10.1021/bi00572a007. [DOI] [PubMed] [Google Scholar]
  17. Straus S. E., Raskas H. J. Transfection of KB cells by polyethylene glycol-induced fusion with erythrocyte ghosts containing adenovirus type 2 DNA. J Gen Virol. 1980 May;48(1):241–245. doi: 10.1099/0022-1317-48-1-241. [DOI] [PubMed] [Google Scholar]
  18. Straus S. E., Sergeant A., Tigges M. A., Raskas H. J. Parental adenovirus type 2 genomes recovered early or late in infection possess terminal proteins. J Virol. 1979 Feb;29(2):828–832. doi: 10.1128/jvi.29.2.828-832.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Thiel J. F., Smith K. O. Fluorescent focus assay of viruses on cell monolayers in plastic Petri plates. Proc Soc Exp Biol Med. 1967 Jul;125(3):892–895. doi: 10.3181/00379727-125-32232. [DOI] [PubMed] [Google Scholar]
  20. Tibbetts C., Johansson K., Philipson L. Hydroxyapatite chromatography and formamide denaturation of adenovirus DNA. J Virol. 1973 Aug;12(2):218–225. doi: 10.1128/jvi.12.2.218-225.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wilson T., Papahadjopoulos D., Taber R. Biological properties of poliovirus encapsulated in lipid vesicles: antibody resistance and infectivity in virus-resistant cells. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3471–3475. doi: 10.1073/pnas.74.8.3471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wilson T., Papahadjopoulos D., Taber R. The introduction of poliovirus RNA into cells via lipid vesicles (liposomes). Cell. 1979 May;17(1):77–84. doi: 10.1016/0092-8674(79)90296-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES