Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1971 Aug;9(2):249–258.

Depression of delayed hypersensitivity by pretreatment with Freund-type adjuvants. I. Description of the phenomenon

G L Asherson, G G Allwood
PMCID: PMC1713023  PMID: 4105803

Abstract

Delayed hypersensitivity induced by antigen in Freund's complete adjuvant (FCA) can be depressed by pretreatment with FCA alone beforehand. In the guinea-pig, pretreatment with FCA depressed the 24 hr skin reactions which otherwise followed immunization with bovine γ-globulin, human serum albumin and the arsanil-N-acetyl tyrosine in FCA. The depression ranged from 25 to 75% and 4 hr skin reactions were also depressed. In contrast, haemolytic and cytophilic antibody to BGG was not depressed by pretreatment with FCA before immunization with antigen in FCA.

It was not necessary to use the same adjuvant for pretreatment and immunization, and pretreatment with FCA or Corynebacterium parvum adjuvant depressed delayed hypersensitivity to BGG induced by BGG in either FCA or C. parvum adjuvant.

In both the guinea-pig and rat, pretreatment with FCA, and soluble antigen acted synergically in depressing 24 hr delayed skin reactions. Thus pretreatment with either FCA or soluble antigen, before immunization with antigen in FCA depressed delayed hypersensitivity skin reactions, and pretreatment with both agents caused a greater depression than either singly. However pretreatment with FCA did not alter the depression of antibody production caused by soluble antigen.

In the mouse, pretreatment with FCA or C. parvum depressed contact sensitivity to picryl chloride induced by picryl chloride in FCA. Contact sensitivity to picryl chloride and oxazolone induced by skin painting was not depressed by pretreatment with FCA.

Full text

PDF
253

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allwood G. G., Asherson G. L. Depression of delayed hypersensitivity by pretreatment with Freund-type adjuvants. II. Mechanism of the phenomenon. Clin Exp Immunol. 1971 Aug;9(2):259–266. [PMC free article] [PubMed] [Google Scholar]
  2. Asherson G. L., Ptak W. Contact and delayed hypersensitivity in the mouse. 3. Depression of contact sensitivity by pre-treatment with antigen and the restoration of immune competence in tolerant mice by normal lymphoid and bone marrow cells. Immunology. 1970 Jan;18(1):99–106. [PMC free article] [PubMed] [Google Scholar]
  3. Asherson G. L. Selective and specific inhibition of 24-hour skin reactions in the guinea-pig. II. The mechanism of immune deviation. Immunology. 1966 Feb;10(2):179–186. [PMC free article] [PubMed] [Google Scholar]
  4. Berman L. D., Allison A. C., Pereira H. G. Effects of Freund's adjuvant on adenovirus oncogenesis and antibody production in hamsters. Int J Cancer. 1967 Sep 15;2(5):539–543. doi: 10.1002/ijc.2910020515. [DOI] [PubMed] [Google Scholar]
  5. DRESSER D. W. Effectiveness of lipid and lipidophilic substances as adjuvants. Nature. 1961 Sep 16;191:1169–1171. doi: 10.1038/1911169a0. [DOI] [PubMed] [Google Scholar]
  6. DVORAK H. F., BILLOTE J. B., MCCARTHY J. S., FLAX M. H. IMMUNOLOGIC UNRESPONSIVENESS IN THE ADULT GUINEA PIG. I. SUPPRESSION OF DELAYED HYPERSENSITIVITY AND ANTIBODY FORMATION TO PROTEIN ANTIGENS. J Immunol. 1965 Jun;94:966–975. [PubMed] [Google Scholar]
  7. Davey M. J., Asherson G. L., Stone S. H. Selective and specific inhibition of 24 hour skin reactions in the guinea-pig. 3. Depression of cytophilic and haemolytic antibodies by pretreatment with antigen and the effect of irradiation. Immunology. 1971 Apr;20(4):513–522. [PMC free article] [PubMed] [Google Scholar]
  8. Goldner H., Girardi A. J., Hilleman M. R. Enhancement in hamsters of virus oncogenesis attending vaccination procedures. Virology. 1965 Oct;27(2):225–227. doi: 10.1016/0042-6822(65)90164-9. [DOI] [PubMed] [Google Scholar]
  9. JANKOVIC B. D. Impairment of immunological reactivity in guinea pigs by prior injection of adjuvant. Nature. 1962 Feb 24;193:789–790. doi: 10.1038/193789a0. [DOI] [PubMed] [Google Scholar]
  10. Jones V. E., Leskowitz S. Immunochemical study of antigenic specificity in delayed hypersensitivity. IV. The production of unresponsiveness to delayed hypersensitivity with a single antigenic determinant. J Exp Med. 1965 Sep 1;122(3):505–515. doi: 10.1084/jem.122.3.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KIES M. W., ALVORD E. C., Jr [Prevention of allergic encephalomyelitis by prior injection of adjuvants]. Nature. 1958 Oct 18;182(4642):1106–1106. doi: 10.1038/1821106a0. [DOI] [PubMed] [Google Scholar]
  12. Lisak R. P., Kies M. W. Mycobacterial suppression of delayed hypersensitivity in experimental allergic encephalomyelitis. Proc Soc Exp Biol Med. 1968 May;128(1):214–218. doi: 10.3181/00379727-128-32981. [DOI] [PubMed] [Google Scholar]
  13. NELSON D. S., BOYDEN S. V. THE CUTANEOUS REACTIVITY OF GUINEA PIGS TO PURE PROTEIN ANTIGENS. I. A CRITICAL EVALUATION OF METHODS FOR THE INDUCTION OF DELAYED-TYPE HYPERSENSITIVITY TO PURE PROTEINS. Int Arch Allergy Appl Immunol. 1964;25:279–303. doi: 10.1159/000229529. [DOI] [PubMed] [Google Scholar]
  14. STAVITSKY A. B. Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells. J Immunol. 1954 May;72(5):360–367. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES