Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1971 Sep;9(3):313–328.

Inhibition of human mixed lymphocyte reaction by antibodies to immunoglobulin light chain determinants

M F Greaves, G Torrigiani, I M Roitt
PMCID: PMC1713068  PMID: 4105838

Abstract

The proliferative response of human blood lymphocytes to homologous cells in vitro (i.e. the mixed lymphocyte reaction) has been used as a model of cell-mediated immune responses to investigate the nature of the antigen receptor site involved in the initiation of these reactions. Pretreatment of lymphocytes with the Fabγ monomer of rabbit antisera to human immunoglobulin light chain determinants effectively suppressed subsequent responsiveness to antigen without impairing lymphocyte viability or response to phytohaemagglutinin. Experiments in which the dose of antigen and the time of addition of antigen relative to antiserum were varied suggested that anti-light chain antibodies compete with antigen for the receptor site. These results are discussed in relation to the problem of the chemical nature of the receptor site for transplantation reactions and delayed hypersensitivity.

Full text

PDF
317

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amos D. B., Bach F. H. Phenotypic expressions of the major histocompatibility locus in man (HL-A): leukocyte antigens and mixed leukocyte culture reactivity. J Exp Med. 1968 Oct 1;128(4):623–637. doi: 10.1084/jem.128.4.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BAIN B., VAS M. R., LOWENSTEIN L. THE DEVELOPMENT OF LARGE IMMATURE MONONUCLEAR CELLS IN MIXED LEUKOCYTE CULTURES. Blood. 1964 Jan;23:108–116. [PubMed] [Google Scholar]
  3. Biozzi G., Binaghi R. A., Stiffel C., Mouton D. Production of different classes of immunoglobulins by individual cells in the guinea-pig. Immunology. 1969 Mar;16(3):349–359. [PMC free article] [PubMed] [Google Scholar]
  4. CEBRA J. J., GIVOL D., SILMAN H. I., KATCHALSKI E. A two-stage cleavage of rabbit gamma-globulin by a water-insoluble papain preparation followed by cysteine. J Biol Chem. 1961 Jun;236:1720–1725. [PubMed] [Google Scholar]
  5. Cheng W. C., Talmage D. W. The reaction of immunoglobulin-coated bentonite and radioiodinated antigen as a basis for antibody detection and antigen binding. J Immunol. 1969 Dec;103(6):1385–1394. [PubMed] [Google Scholar]
  6. DAVID J. R., AL-ASKARI S., LAWRENCE H. S., THOMAS L. DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS. J Immunol. 1964 Aug;93:264–273. [PubMed] [Google Scholar]
  7. DECARVALHO S., LEWIS A. J., RAND H. J., UHRICK J. R. IMMUNOCHROMATOGRAPHIC PARTITION OF SOLUBLE ANTIGENS ON COLUMNS OF INSOLUBLE DIAZO-GAMMA-GLOBULINS. Nature. 1964 Oct 17;204:265–266. doi: 10.1038/204265a0. [DOI] [PubMed] [Google Scholar]
  8. FLEISCHMAN J. B., PAIN R. H., PORTER R. R. Reduction of gamma-globulins. Arch Biochem Biophys. 1962 Sep;Suppl 1:174–180. [PubMed] [Google Scholar]
  9. FULTHORPE A. J., ROITT I. M., DONIACH D., COUCHMAN K. A stable sheep cell preparation for detecing thyroglobulin auto-antibodies and its clinical applications. J Clin Pathol. 1961 Nov;14:654–660. doi: 10.1136/jcp.14.6.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Falk R. E., Thorsby E., Möller E., Möller G. In vitro assay of cell-mediated immunity: the inhibition of migration of sensitized human lymphocytes by HL-A antigens. Clin Exp Immunol. 1970 Apr;6(4):445–455. [PMC free article] [PubMed] [Google Scholar]
  11. Greaves M. F. Biological effects of anti-immunoglobulins: evidence for immunoglobulin receptors on 'T' and 'B' lymphocytes. Transplant Rev. 1970;5:45–75. doi: 10.1111/j.1600-065x.1970.tb00356.x. [DOI] [PubMed] [Google Scholar]
  12. Greaves M. F., Roitt I. M., Zamir R., Carnaghan R. B. Effect of anti-lymphocyte serum on responses of human peripheral-blood lymphocytes to specific and non-specific stimulants in vitro. Lancet. 1967 Dec 23;2(7530):1317–1319. doi: 10.1016/s0140-6736(67)90909-9. [DOI] [PubMed] [Google Scholar]
  13. Greaves M. F., Torrigiani G., Roitt I. M. Blocking of the lymphocyte receptor site for cell mediated hypersensitivity and transplantation reactions by anti-light chain sera. Nature. 1969 May 31;222(5196):885–886. doi: 10.1038/222885a0. [DOI] [PubMed] [Google Scholar]
  14. HIRSCHHORN K., BACH F., RAPAPORT F. T., CONVERSE J. M., LAWRENCE H. S. THE RELATIONSHIP OF IN VITRO LYMPHOCYTE COMPATIBILITY TO HOMOGRAFT SENSITIVITY IN MAN. Ann N Y Acad Sci. 1964 Nov 30;120:303–306. doi: 10.1111/j.1749-6632.1964.tb34729.x. [DOI] [PubMed] [Google Scholar]
  15. Johnston J. M., Wilson D. B. Origin of immunoreactive lymphocytes in rats. Cell Immunol. 1970 Oct;1(4):430–444. doi: 10.1016/0008-8749(70)90019-5. [DOI] [PubMed] [Google Scholar]
  16. Mason S., Warner N. L. The immunoglobulin nature of the antigen recognition site on cells mediating transplantation immunity and delayed hypersentivity. J Immunol. 1970 Mar;104(3):762–765. [PubMed] [Google Scholar]
  17. Mauel J., Rudolf H., Chapuis B., Brunner K. T. Studies of allograft immunity in mice. II. Mechanism of target cell inactivation in vitro by sensitized lymphocytes. Immunology. 1970 Apr;18(4):517–535. [PMC free article] [PubMed] [Google Scholar]
  18. McConnell I., Munro A., Gurner B. W., Coombs R. R. Studies on actively allergized cells. I. The cyto-dynamics and morphology of rosete-forming lymph node cells in mice and inhibition of rosette-formation with antibody to mouse immunoglobulins. Int Arch Allergy Appl Immunol. 1969;35(3):209–227. [PubMed] [Google Scholar]
  19. Nisbet N. W., Simonsen M., Zaleski M. The frequency of antigen-sensitive cells in tissue transplantation. A commentary on clonal selection. J Exp Med. 1969 Mar 1;129(3):459–467. doi: 10.1084/jem.129.3.459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Perlmann P., Holm G. Cytotoxic effects of lymphoid cells in vitro. Adv Immunol. 1969;11:117–193. doi: 10.1016/s0065-2776(08)60479-4. [DOI] [PubMed] [Google Scholar]
  21. Raff M. C. Two distinct populations of peripheral lymphocytes in mice distinguishable by immunofluorescence. Immunology. 1970 Oct;19(4):637–650. [PMC free article] [PubMed] [Google Scholar]
  22. Robbins J. B., Haimovich J., Sela M. Purification of antibodies with immunoadsorbents prepared using bromoacetyl cellulose. Immunochemistry. 1967 Jan;4(1):11–22. doi: 10.1016/0019-2791(67)90192-9. [DOI] [PubMed] [Google Scholar]
  23. Rode H. N., Gordon J. The mixed leukocyte culture: a three component system. J Immunol. 1970 Jun;104(6):1453–1457. [PubMed] [Google Scholar]
  24. Roitt I. M., Greaves M. F., Torrigiani G., Brostoff J., Playfair J. H. The cellular basis of immunological responses. A synthesis of some current views. Lancet. 1969 Aug 16;2(7616):367–371. doi: 10.1016/s0140-6736(69)92712-3. [DOI] [PubMed] [Google Scholar]
  25. Schellekens P. T., Vriesendorp B., Eijsvoogel V. P., Van Leeuwen A., Van Rood J. J., Miggiano V., Ceppellini R. Lymphocyte transformation in vitro. II. Mixed lymphocyte culture in relation to leucocyte antigens. Clin Exp Immunol. 1970 Feb;6(2):241–254. [PMC free article] [PubMed] [Google Scholar]
  26. Siskind G. W., Benacerraf B. Cell selection by antigen in the immune response. Adv Immunol. 1969;10:1–50. doi: 10.1016/s0065-2776(08)60414-9. [DOI] [PubMed] [Google Scholar]
  27. Sulitzeanu D. Affinity of antigen for white cells and its relation to the induction of antibody formation. Bacteriol Rev. 1968 Dec;32(4 Pt 2):404–424. [PMC free article] [PubMed] [Google Scholar]
  28. TAN M., EPSTEIN W. V. A DIRECT IMMUNOLOGIC ASSAY OF HUMAN SERA FOR BENCE JONES PROTEINS (L-CHAINS). J Lab Clin Med. 1965 Aug;66:344–356. [PubMed] [Google Scholar]
  29. Torrigiani G., Ansell B. M., Chown E. E., Roitt I. M. Raised IgG antiglobulin factors in Still's disease. Ann Rheum Dis. 1969 Jul;28(4):424–427. doi: 10.1136/ard.28.4.424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Twomey J. J., Sharkey O., Jr, Brown J. A., Laughter A. H., Jordan P. H., Jr Cellular requirements for the mitotic response in allogeneic mixed leukocyte cultures. J Immunol. 1970 Apr;104(4):845–853. [PubMed] [Google Scholar]
  31. Wilson D. B., Blyth JL NOWELL P. C. Quantitative studies on the mixed lymphocyte interaction in rats. 3. Kinetics of the response. J Exp Med. 1968 Nov 1;128(5):1157–1181. doi: 10.1084/jem.128.5.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wilson D. B., Nowell P. C. Quantitative studies on the mixed lymphocyte interaction in rats. IV. Immunologic potentiality of the responding cells. J Exp Med. 1970 Feb;131(2):391–407. doi: 10.1084/jem.131.2.391. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES