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. 1972 Jan;10(1):171–177.

The human secondary immune response to keyhole limpet haemocyanin

J E Curtis, E M Hersh
PMCID: PMC1713091  PMID: 5017367

Abstract

The human secondary immune response to the protein antigen Keyhole limpet haemocyanin (KLH) was studied in thirteen normal adults. For secondary immunization and for measurement of delayed hypersensitivity, 100 μg of KLH was given intradermally to each individual between 81 and 375 days after primary immunization. Immunity was assessed by delayed hypersensitivity skin testing and measurement of antibody formation and KLH stimulated in vitro lymphocyte blastogenesis. Twelve of the thirteen subjects had positive KLH delayed hypersensitivity reactions. Prior to secondary immunization all thirteen had circulating lymphocytes responsive in vitro to KLH and all had circulating haemagglutinating antibody. After secondary immunization there was no further increase in the mean KLH-stimulated blastogenesis while the KLH haemagglutinin titre rose due to increased 7S antibody formation. Primary immunization with small doses of KLH was as effective in inducing immunological memory and a subsequent secondary response as immunization with large doses of the antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOREL Y., FAUCONNET M., MIESCHER P. A. 7S VERSUS 19S ANAMNESTIC RESPONSE IN RABBITS. Proc Soc Exp Biol Med. 1964 Nov;117:603–607. doi: 10.3181/00379727-117-29649. [DOI] [PubMed] [Google Scholar]
  2. Boyden S. V. Natural antibodies and the immune response. Adv Immunol. 1966;5:1–28. doi: 10.1016/s0065-2776(08)60271-0. [DOI] [PubMed] [Google Scholar]
  3. Curtis J. E., Hersh E. M., Harris J. E., McBride C., Freireich E. J. The human primary immune response to keyhole limpet haemocyanin: interrelationships of delayed hypersensitivity, antibody response and in vitro blast transformation. Clin Exp Immunol. 1970 Apr;6(4):473–491. [PMC free article] [PubMed] [Google Scholar]
  4. DEUTSCH H. F., MORTON J. I. Dissociation of human serum macroglobulins. Science. 1957 Mar 29;125(3248):600–601. doi: 10.1126/science.125.3248.600. [DOI] [PubMed] [Google Scholar]
  5. Gleich G. J., Uhr J. W., Vaughan J. H., Swedlund H. A. Antibody formation in dysgammaglobulinemia. J Clin Invest. 1966 Aug;45(8):1334–1340. doi: 10.1172/JCI105440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hersh E. M., Brown B. W. Inhibition of immune responses by glutamine antagonism: effect of azotomycin on lymphocyte blastogenesis. Cancer Res. 1971 Jun;31(6):834–840. [PubMed] [Google Scholar]
  7. Hughes N. R. Tuberculin sensitive lymphocytes in human peripheral blood following Mantoux test. Aust J Exp Biol Med Sci. 1968 Oct;46(5):619–629. doi: 10.1038/icb.1968.168. [DOI] [PubMed] [Google Scholar]
  8. Maurer P. H. Antigenicity of polypeptides (poly-alpha-amino acids). XVII. Immunologic studies in humans with polymers containing L or D and L-alpha-amino acids. J Immunol. 1965 Dec;95(6):1095–1099. [PubMed] [Google Scholar]
  9. Nossal G. J., Austin C. M., Ada G. L. Antigens in immunity. VII. Analysis of immunological memory. Immunology. 1965 Oct;9(4):333–348. [PMC free article] [PubMed] [Google Scholar]
  10. Rowley M. J. "Natural" antibody in man to flagellar antigens of Salmonella adelaide. Aust J Exp Biol Med Sci. 1970 Apr;48(2):249–252. doi: 10.1038/icb.1970.26. [DOI] [PubMed] [Google Scholar]
  11. Rowley M. J., Mackay I. R. Measurement of antibody-producing capacity in man. I. The normal response to flagellin from Salmonella adelaide. Clin Exp Immunol. 1969 Oct;5(4):407–418. [PMC free article] [PubMed] [Google Scholar]
  12. Salvaggio J., Castro-Murillo E., Kundur V. Immunologic response of atopic and normal individuals to keyhole limpet hemocyanin. J Allergy. 1969 Dec;44(6):344–354. doi: 10.1016/0021-8707(69)90026-4. [DOI] [PubMed] [Google Scholar]
  13. Smith J. B., Cunningham A. J., Lafferty K. J., Morris B. The role of the lymphatic system and lymphoid cells in the establishment of immunological memory. Aust J Exp Biol Med Sci. 1970 Feb;48(1):57–70. doi: 10.1038/icb.1970.6. [DOI] [PubMed] [Google Scholar]
  14. Stewart C. J. Skin sensitivity to human, avian and BCG PPDs after BCG vaccination. Tubercle. 1968 Mar;49(1):84–91. doi: 10.1016/s0041-3879(68)80010-8. [DOI] [PubMed] [Google Scholar]
  15. Swanson M. A., Schwartz R. S. Immunosuppressive therapy. The relation between clinical response and immunologic competence. N Engl J Med. 1967 Jul 27;277(4):163–170. doi: 10.1056/NEJM196707272770401. [DOI] [PubMed] [Google Scholar]
  16. Uhr J. W., Finkelstein M. S. The kinetics of antibody formation. Prog Allergy. 1967;10:37–83. [PubMed] [Google Scholar]

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