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. 1972 Apr;10(4):685–696.

The role of immunoconglutinin in the in vivo and in vitro destruction of red cells

F Tedesco, R Corrocher, D L Brown
PMCID: PMC1713106  PMID: 5034249

Abstract

Two experimental approaches were used to investigate the role of immunoconglutinin (IK) in the in vivo destruction of red cells in rabbits. In a first series of experiments the behaviour of EC43 (C3-coated red cells) was followed in IK-producing rabbits and in rabbits passively receiving IK, both of which had previously been complement-depleted by cobra venom factor (CoF). The red cells were sequestered to a minor degree and returned to the circulation within 20–30 min, whereas in the normal control, EC43 returned to the circulation over a period of 3–4 hr. In contrast to EC43, EC43IK (IK-coated EC43) did not form rosettes around the Kupffer cells, suggesting that IK blocks the functional activity of C3 and so interferes with the interaction between C3 and C3-receptors on fixed macrophages. In a second series of experiments EC43 and EC43IK, injected into IK-producing rabbits and normal rabbits respectively, underwent marked lysis and erythrophagocytosis. Examination of liver imprint preparations from these rabbits revealed rosette formation around the Kupffer cells, indicating the fixation of more C4 and C3 by bound IK.

In vitro experiments confirmed both the inhibitory activity and the complement-fixing capacity of IK.

The results suggest that IK normally has an amplifying effect on complement fixation in vivo and so potentiates the ability of complement to bring about red-cell destruction.

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These references are in PubMed. This may not be the complete list of references from this article.

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