Abstract
By means of the antigen–antibody crossed electrophoresis of Laurell twenty-two different Candida albicans precipitins were demonstrated in a serum from a 56-year-old woman suffering from idiopathic hypoparathyroidism and chronic candidiasis. This precipitin response is remarkably strong, but a detailed evaluation of the finding must await further investigations.
It is mentioned how the human precipitins may be quantitated and the titres expressed in arbitrary rabbit precipitin units.
The antigen–antibody crossed electrophoresis may be useful in other infections, and possibly in certain autoimmune diseases, in order quantitatively to screen patient sera for a multitude of precipitins without purification of the individual antigens.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Clarke H. G., Freeman T. Quantitative immunoelectrophoresis of human serum proteins. Clin Sci. 1968 Oct;35(2):403–413. [PubMed] [Google Scholar]
- Coudert J., Truong K., Ambroise-Thomas P., Douchet C. Etudes antigéniques comparées de quelques levures pathogènes du genre Candida par immunofluorescence, agglutination et immunoélectrophorèse. Ann Inst Pasteur (Paris) 1968 Mar;114(3):287–296. [PubMed] [Google Scholar]
- LAURELL C. B. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. Anal Biochem. 1965 Feb;10:358–361. doi: 10.1016/0003-2697(65)90278-2. [DOI] [PubMed] [Google Scholar]
- Pepys J., Faux J. A., McCarthy D. S., Hargreave F. E. Candida albicans precipitins in respiratory disease in man. J Allergy. 1968 Jun;41(6):305–318. doi: 10.1016/0021-8707(68)90073-7. [DOI] [PubMed] [Google Scholar]
- SVANE-KNUDSEN P. Severe secondary ocular changes in a patient suffering from idiopathic hypoparathyroidism and pernicious anaemia. Acta Ophthalmol (Copenh) 1959;37:560–567. doi: 10.1111/j.1755-3768.1959.tb03469.x. [DOI] [PubMed] [Google Scholar]
- Taschdjian C. L., Cuesta M. B., Kozinn P. J., Caroline L. A modified antigen for serodiagnosis of systemic candidiasis. Am J Clin Pathol. 1969 Oct;52(4):468–472. doi: 10.1093/ajcp/52.4.468. [DOI] [PubMed] [Google Scholar]
- Weeke B. The serum proteins identified by means of the Laurell crossed electrophoresis. Scand J Clin Lab Invest. 1970 May;25(3):269–275. doi: 10.3109/00365517009046205. [DOI] [PubMed] [Google Scholar]