Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1981 Aug;39(2):341–347. doi: 10.1128/jvi.39.2.341-347.1981

Poliovirus morphogenesis. I. Identification of 80S dissociable particles and evidence for the artifactual production of procapsids.

M E Marongiu, A Pani, M V Corrias, M Sau, P La Colla
PMCID: PMC171342  PMID: 6268826

Abstract

The current model of poliovirus morphogenesis postulates a fundamental role for procapsid, 80S shells that, upon interaction with viral RNA and subsequent proteolytic cleavage, give rise to complete virus particles. Although 80S sedimenting particles can, indeed, be isolated from cytoplasmic extracts of infected cells, their physical properties differ from those reported for procapsids. Far from being stable structures, they can be dissociated by pH 8.5 and 0.1% sodium dodecyl sulfate into slower-sedimenting subunits. The reasons for this discrepancy were investigated, and two main modalities leading to the appearance of procapsids in vitro were identified. The first involves a temperature-mediated conversion of dissociable 80S particles into stable 80S procapsids, and the second involves the self-assembly of endogenous 14S subunits, also primed by an increase in the temperature of cytoplasmic extracts.

Full text

PDF
342

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caliguiri L. A., Mosser A. G. Proteins associated with the poliovirus RNA replication complex. Virology. 1971 Nov;46(2):375–386. doi: 10.1016/0042-6822(71)90039-0. [DOI] [PubMed] [Google Scholar]
  2. Fernandez-Tomas C. B., Baltimore D. Morphogenesis of poliovirus. II. Demonstration of a new intermediate, the proviron. J Virol. 1973 Nov;12(5):1122–1130. doi: 10.1128/jvi.12.5.1122-1130.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ghendon Y., Yakobson E., Mikhejeva A. Study of some stages of poliovirus morphogenesis in MiO cells. J Virol. 1972 Aug;10(2):261–266. doi: 10.1128/jvi.10.2.261-266.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HUMMELER K., ANDERSON T. F., BROWN R. A. Identification of poliovirus particles of different antigenicity by specific agglutination as seen in the electron microscope. Virology. 1962 Jan;16:84–90. doi: 10.1016/0042-6822(62)90205-2. [DOI] [PubMed] [Google Scholar]
  5. Jacobson M. F., Asso J., Baltimore D. Further evidence on the formation of poliovirus proteins. J Mol Biol. 1970 May 14;49(3):657–669. doi: 10.1016/0022-2836(70)90289-5. [DOI] [PubMed] [Google Scholar]
  6. Jacobson M. F., Baltimore D. Morphogenesis of poliovirus. I. Association of the viral RNA with coat protein. J Mol Biol. 1968 Apr 28;33(2):369–378. doi: 10.1016/0022-2836(68)90195-2. [DOI] [PubMed] [Google Scholar]
  7. Lee P. W., Colter J. S. Further characterization of Mengo subviral particles: a new hypothesis for picornavirus assembly. Virology. 1979 Sep;97(2):266–274. doi: 10.1016/0042-6822(79)90338-6. [DOI] [PubMed] [Google Scholar]
  8. Lee P. W., Paucha E., Colter J. S. Identification and partial characterization of a new (50 S) subviral particle in Mengo virus-infected L cells. Virology. 1978 Mar;85(1):286–295. doi: 10.1016/0042-6822(78)90432-4. [DOI] [PubMed] [Google Scholar]
  9. Phillips B. A. In vitro assembly of poliovirus. II. Evidence for the self-assembly of 14 S particles into empty capsids. Virology. 1971 May;44(2):307–316. doi: 10.1016/0042-6822(71)90262-5. [DOI] [PubMed] [Google Scholar]
  10. Phillips B. A. In vitro assembly of polioviruses. I. Kinetics of the assembly of empty capsids and the role of extracts from infected cells. Virology. 1969 Dec;39(4):811–821. doi: 10.1016/0042-6822(69)90018-x. [DOI] [PubMed] [Google Scholar]
  11. Phillips B. A., Summers D. F., Maizel J. V., Jr In vitro assembly of poliovirus-related particles. Virology. 1968 Jun;35(2):216–226. doi: 10.1016/0042-6822(68)90262-6. [DOI] [PubMed] [Google Scholar]
  12. Phillips B. A., Wiemert S. In vitro assembly of poliovirus. V. Evidence that the self-assembly activity of 14 S particles is independent of extract assembly factor(s) and host proteins. Virology. 1978 Jul 1;88(1):92–104. doi: 10.1016/0042-6822(78)90113-7. [DOI] [PubMed] [Google Scholar]
  13. SCHARFF M. D., LEVINTOW L. Quantitative study of the formation of poliovirus antigens in infected HeLa cells. Virology. 1963 Apr;19:491–500. doi: 10.1016/0042-6822(63)90043-6. [DOI] [PubMed] [Google Scholar]
  14. Yin F. H. Involvement of viral procapsid in the RNA synthesis and maturation of poliovirus. Virology. 1977 Oct 15;82(2):299–307. doi: 10.1016/0042-6822(77)90005-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES