Skip to main content
PMC home page
American Journal of Human Genetics logoLink to American Journal of Human Genetics
. 1988 Feb;42(2):335–344.

Isolation of DNA markers in the direction of the Huntington disease gene from the G8 locus.

B Smith 1, D Skarecky 1, U Bengtsson 1, R E Magenis 1, N Carpenter 1, J J Wasmuth 1
PMCID: PMC1715250  PMID: 2963539

Abstract

To facilitate identification of additional DNA markers near and on opposite sides of the Huntington disease (HD) gene, we developed a panel of somatic-cell hybrids that allows accurate subregional mapping of DNA fragments in the distal portion of 4p. By means of the hybrid-cell mapping panel and a library of DNA fragments enriched for sequences from the terminal one-third of the short arm of chromosome 4, 105 DNA fragments were mapped to six different physical regions within 4p15-4pter. Four polymorphic DNA fragments of particular interest were identified, at least three of which are distal to the HD-linked D4S10 (G8) locus, a region of 4p previously devoid of DNA markers. Since the HD gene has also recently been shown to be distal to G8, these newly identified DNA markers are in the direction of the HD gene from G8, and one or more of them may be on the opposite side of HD from G8.

Full text

PDF
344

Images in this article

338Figure 1
on p.338
339Figure 2
on p.339
341Figure 5
on p.341

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carlock L. R., Smith D., Wasmuth J. J. Genetic counterselective procedure to isolate interspecific cell hybrids containing single human chromosomes: construction of cell hybrids and recombinant DNA libraries specific for human chromosomes 3 and 4. Somat Cell Mol Genet. 1986 Mar;12(2):163–174. doi: 10.1007/BF01560663. [DOI] [PubMed] [Google Scholar]
  2. Dana S., Wasmuth J. J. Selective linkage disruption in human-Chinese hamster cell hybrids: deletion mapping of the leuS, hexB, emtB, and chr genes on human chromosome 5. Mol Cell Biol. 1982 Oct;2(10):1220–1228. doi: 10.1128/mcb.2.10.1220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Estivill X., Farrall M., Scambler P. J., Bell G. M., Hawley K. M., Lench N. J., Bates G. P., Kruyer H. C., Frederick P. A., Stanier P. A candidate for the cystic fibrosis locus isolated by selection for methylation-free islands. 1987 Apr 30-May 6Nature. 326(6116):840–845. doi: 10.1038/326840a0. [DOI] [PubMed] [Google Scholar]
  4. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  5. Friend S. H., Bernards R., Rogelj S., Weinberg R. A., Rapaport J. M., Albert D. M., Dryja T. P. A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma. Nature. 1986 Oct 16;323(6089):643–646. doi: 10.1038/323643a0. [DOI] [PubMed] [Google Scholar]
  6. Gilliam T. C., Tanzi R. E., Haines J. L., Bonner T. I., Faryniarz A. G., Hobbs W. J., MacDonald M. E., Cheng S. V., Folstein S. E., Conneally P. M. Localization of the Huntington's disease gene to a small segment of chromosome 4 flanked by D4S10 and the telomere. Cell. 1987 Aug 14;50(4):565–571. doi: 10.1016/0092-8674(87)90029-8. [DOI] [PubMed] [Google Scholar]
  7. Gusella J. F., Tanzi R. E., Bader P. I., Phelan M. C., Stevenson R., Hayden M. R., Hofman K. J., Faryniarz A. G., Gibbons K. Deletion of Huntington's disease-linked G8 (D4S10) locus in Wolf-Hirschhorn syndrome. Nature. 1985 Nov 7;318(6041):75–78. doi: 10.1038/318075a0. [DOI] [PubMed] [Google Scholar]
  8. Gusella J. F., Wexler N. S., Conneally P. M., Naylor S. L., Anderson M. A., Tanzi R. E., Watkins P. C., Ottina K., Wallace M. R., Sakaguchi A. Y. A polymorphic DNA marker genetically linked to Huntington's disease. Nature. 1983 Nov 17;306(5940):234–238. doi: 10.1038/306234a0. [DOI] [PubMed] [Google Scholar]
  9. Landegent J. E., Jansen in de Wal N., Fisser-Groen Y. M., Bakker E., van der Ploeg M., Pearson P. L. Fine mapping of the Huntington disease linked D4S10 locus by non-radioactive in situ hybridization. Hum Genet. 1986 Aug;73(4):354–357. doi: 10.1007/BF00279100. [DOI] [PubMed] [Google Scholar]
  10. Magenis R. E., Gusella J., Weliky K., Olson S., Haight G., Toth-Fejel S., Sheehy R. Huntington disease-linked restriction fragment length polymorphism localized within band p16.1 of chromosome 4 by in situ hybridization. Am J Hum Genet. 1986 Sep;39(3):383–391. [PMC free article] [PubMed] [Google Scholar]
  11. Monaco A. P., Neve R. L., Colletti-Feener C., Bertelson C. J., Kurnit D. M., Kunkel L. M. Isolation of candidate cDNAs for portions of the Duchenne muscular dystrophy gene. Nature. 1986 Oct 16;323(6089):646–650. doi: 10.1038/323646a0. [DOI] [PubMed] [Google Scholar]
  12. Overhauser J., Beaudet A. L., Wasmuth J. J. A fine structure physical map of the short arm of chromosome 5. Am J Hum Genet. 1986 Nov;39(5):562–572. [PMC free article] [PubMed] [Google Scholar]
  13. Overhauser J., Golbus M. S., Schonberg S. A., Wasmuth J. J. Molecular analysis of an unbalanced deletion of the short arm of chromosome 5 that produces no phenotype. Am J Hum Genet. 1986 Jul;39(1):1–10. [PMC free article] [PubMed] [Google Scholar]
  14. Royer-Pokora B., Kunkel L. M., Monaco A. P., Goff S. C., Newburger P. E., Baehner R. L., Cole F. S., Curnutte J. T., Orkin S. H. Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location. Nature. 1986 Jul 3;322(6074):32–38. doi: 10.1038/322032a0. [DOI] [PubMed] [Google Scholar]
  15. Sealey P. G., Whittaker P. A., Southern E. M. Removal of repeated sequences from hybridisation probes. Nucleic Acids Res. 1985 Mar 25;13(6):1905–1922. doi: 10.1093/nar/13.6.1905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stanley W., Chu E. H. Assignment of the gene for phosphoribosylpyrophosphate amidotransferase to the pter leads to q21 region of human chromosome 4. Cytogenet Cell Genet. 1978;22(1-6):228–231. doi: 10.1159/000130943. [DOI] [PubMed] [Google Scholar]
  17. Wang H. S., Greenberg C. R., Hewitt J., Kalousek D., Hayden M. R. Subregional assignment of the linked marker G8 (D4S10) for Huntington disease to chromosome 4p16.1-16.3. Am J Hum Genet. 1986 Sep;39(3):392–396. [PMC free article] [PubMed] [Google Scholar]
  18. Wasmuth J. J., Carlock L. R., Smith B., Immken L. L. A cell hybrid and recombinant DNA library that facilitate identification of polymorphic loci in the vicinity of the Huntington disease gene. Am J Hum Genet. 1986 Sep;39(3):397–403. [PMC free article] [PubMed] [Google Scholar]
  19. Wexler N. S., Young A. B., Tanzi R. E., Travers H., Starosta-Rubinstein S., Penney J. B., Snodgrass S. R., Shoulson I., Gomez F., Ramos Arroyo M. A. Homozygotes for Huntington's disease. Nature. 1987 Mar 12;326(6109):194–197. doi: 10.1038/326194a0. [DOI] [PubMed] [Google Scholar]

Articles from American Journal of Human Genetics are provided here courtesy of American Society of Human Genetics

RESOURCES